Neighbor-Net network analysis among CaMV isolates based on ORF VI and performed using SplitsTree4.

<p>Formation of a reticular network is suggestive of recombination. Horseradish latent virus (JX429923) is used as the outgroup. Position of 219–1d isolate in Iran I cluster is marked.</p

Viability comparison of CaMV-infected and noninfected algae cultures.

<p>Heterogeneity of F_V/F_M in experimental infected a) and control b) cultures. False color scale—black/blue correspond to the lowest values and orange/red to the highest ones. Higher values indicate a better physiological state of the culture. Resolution is 0.046 mm² per pixel. Detection size is 0.21 mm.</p

Immunodetection of CaMV with colloidal gold in <i>Trebouxia aggregata</i> alga.

<p>Thin sections were prepared from plate-growing algal cells, treated with rabbit anti-CaMV antibody, then with a gold-conjugated anti-rabbit IgG, contrasted with uranyl acetate and examined with a Jeol JEM-1010 electron microscope. 10 nm particles were detected in cytoplasma adjacent to cell, and very rarely in central chloroplast. Bars = 500 nm, CW = cell wall, CH = chloroplast.</p

List of algal strains used in this work.

<p>− = negative, + = positive, ± = intermediate</p><p>List of algal strains used in this work.</p

Presence of SMC domain in members of putative Fusariviridae family.

<p>Position of the SMC domain on the ORF2 and E-value is presented.</p

Comparative codon-usage analysis of viruses from <i>S</i>. <i>alkalinus</i>.

<p>Frequency of [XYG + XYC] of <i>S</i>. <i>alkalinus</i> was computed from codon frequencies of RPB2 and TEF1-alpha protein coding genes sequenced previously [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0187799#pone.0187799.ref001" target="_blank">1</a>], data for <i>Colletotrichum</i> sp. were from <a href="http://www.kazusa.or.jp/" target="_blank">http://www.kazusa.or.jp</a>.</p

Amino acid sequence identities (in %) of ORF1 (under the diagonal) and ORF2 (above the diagonal) between the SaFV1-containing isolates of <i>S</i>. <i>alkalinus</i>.

<p>Amino acid sequence identities (in %) of ORF1 (under the diagonal) and ORF2 (above the diagonal) between the SaFV1-containing isolates of <i>S</i>. <i>alkalinus</i>.</p

Phylogenetic tree of selected members of prokaryotic, eukaryotic, and viral SMC domains.

<p>Phylogenetic ML-tree with Poisson correction with 1000 replications generated from SMC and SMC-like amino acid sequences. sequences (all used organisms and accession numbers of the sequences are shown in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0187799#pone.0187799.s003" target="_blank">S3 Table</a>). Bootstrap values are given at the nodes.</p

The alkalophilic fungus <i>Sodiomyces alkalinus</i> hosts beta- and gammapartitiviruses together with a new fusarivirus - Fig 3

<p>Phylogeny of the <i>Sodiomyces</i> sp. based on the ITS rDNA sequences (A), with the origin location of dsRNA-containing strains and phylogeny of the SaFV1 isolates based on the whole genome sequence (B).</p

Schematic representation of the putative genomic organization of fusariviruses.

<p>The boxes and lines represent open reading frames (ORFs) and non-coding sequences, respectively. Positions of polymerase (Pol, red), helicase (Hel, green), and structural maintenance of chromosomes (SMC, blue) domains are indicated. Genomes are shown to scale. The A(n) represents poly (A) tail. Sodiomyces alkalinus fusarivirus 1 –SaFV1, Fusarium graminearum virus—FgV1, Sclerotinia sclerotiorum fusarivirus 1—SsFV1, Alternaria brassicicola fusarivirus 1- AbFV1,—Nigrospora oryzae fusarivirus 1—NoFV1. Fusarium poae fusarivirus 1 –FpFV1, Pleospora typhicola fusarivirus 1 –PtFV1, Rosellinia necatrix fusarivirus 1 –RnFV1, Penicillium roqueforti ssRNA mycovirus 1 –PrRV1, Macrophomina phaseolina ssRNA virus 1 –MpRV1, and Penicilium aurantiogriseum fusarivirus 1 –PaFV1, have similar genome organization as SaFV1.</p