Association of osteopontin with specific prognostic factors and survival in adjuvant breast cancer trials of the Hellenic Cooperative Oncology Group

Additional file 1. OPN supplementary data

CONTRIBUTION TO THE STUDY OF THE TREATMENT OF MALIGNANT MAMMARY TUMOURS IN THE DOG

IN BITCHES WITH MALIGNANT MAMMARY TUMOURS (CARCINOMAS OR MALIGNANT MIXED TUMOURS) A COMPARISON OF SIMPLE MASTECTOMY AND MODIFIED RADICAL MASTECTOMY WAS MADE. MOREOVER, THE CONTRIBUTION OF A SPECIFIC CHEMOTHERAPEUTIC PROTOCOL (A COMBINATION OF 5 - FLUOROURACIL AND CYCLOPHOSPHAMIDE), GIVEN AS PROPHYLACTIC CHEMOTHERAPY, AS WELL AS ITS SIDE EFFECTS, WERE STUDIED. THE RESULTS OBTAINED WERE THE FOLLOWING: A) NONE OF THE TWO TYPES OF SURGICAL EXCISION HAD A SIGNIFICANT EFFECT (P>0,05) ON THE DISEASE - FREE INTERVAL OR THE SURVIVAL TIME. MAMMARY TUMOURS IN THE DOG MUST BE REMOVED BY THE SIMPLEST PROCEDURE. B) CHEMOTHERAPY CAUSED A SIGNIFICANT (P0,05) ΤΗΣ ΑΛΛΗΣ ΟΣΟΝ ΑΦΟΡΑ ΣΤΗΝ ΑΥΞΗΣΗ ΤΟΥ ΕΛΕΥΘΕΡΟΥ ΑΠΟ ΤΗ ΝΟΣΟ ΧΡΟΝΙΚΟΥ ΔΙΑΣΤΗΜΑΤΟΣ ΚΑΙ ΤΟΥ ΧΡΟΝΟΥ ΕΠΙΒΙΩΣΗΣ. ΤΟ ΝΕΟΠΛΑΣΜΑ ΕΙΝΑΙ ΠΡΟΤΙΜΟΤΕΡΟ ΝΑ ΑΦΑΙΡΕΙΤΑΙ ΜΕ ΤΗΝ ΟΣΟ ΤΟ ΔΥΝΑΤΟΝ ΑΠΛΟΥΣΤΕΡΗ ΤΕΧΝΙΚΗ. Β) Η ΧΗΜΕΙΟΘΕΡΑΠΕΙΑ ΠΡΟΚΑΛΕΣΕ ΣΤΑΤΙΣΤΙΚΩΣ ΣΗΜΑΝΤΙΚΗ ΑΥΞΗΣΗ (Ρ<=0,05) ΤΟΥ ΕΛΕΥΘΕΡΟΥ ΑΠΟ ΤΗ ΝΟΣΟ ΧΡΟΝΙΚΟΥ ΔΙΑΣΤΗΜΑΤΟΣ ΜΟΝΟ ΣΤΑ ΖΩΑ ΜΕ ΜΙΚΤΑ ΚΑΚΟΗΘΗ ΝΕΟΠΛΑΣΜΑΤΑ. ΕΠΙΣΗΣ, ΣΤΑΤΙΣΤΙΚΩΣ ΣΗΜΑΝΤΙΚΑ (Ρ<=0,05) ΑΥΞΗΘΗΚΕ ΚΑΙ Ο ΧΡΟΝΟΣ ΕΠΙΒΙΩΣΗΣ ΤΩΝ ΖΩΩΝ ΜΕ ΜΙΚΤΑ ΚΑΚΟΗΘΗ ΝΕΟΠΛΑΣΜΑΤΑ ΠΟΥ ΗΤΑΝ ΣΤΟ ΙΙΙ ΣΤΑΔΙΟ ΤΗΣΝΟΣΟΥ. ΠΑΡΕΝΕΡΓΕΙΕΣ ΔΕΝ ΠΑΡΑΤΗΡΗΘΗΚΑΝ ΚΛΙΝΙΚΑ. ΚΑΤΑ ΤΗ ΔΙΑΡΚΕΙΑ ΤΗΣ ΧΗΜΕΙΟΘΕΡΑΠΕΙΑΣ ΕΙΝΑΙ ΑΠΑΡΑΙΤΗΤΟ ΝΑ ΓΙΝΕΤΑΙ ΤΟΥΛΑΧΙΣΤΟΝ ΚΑΤΑΜΕΤΡΗΣΗ ΤΩΝ ΛΕΥΚΩΝ ΑΙΜΟΣΦΑΙΡΙΩΝ ΣΕ ΕΒΔΟΜΑΔΙΑΙΑ ΔΙΑΣΤΗΜΑΤΑ

Evaluation of the effectiveness of an ointment based on Alkannins/Shikonins on second intention wound healing in the dog

The enantiomeric naphthoquinones alkannins and shikonins (A/S) have been established as potent wound healing agents. The purpose of this study was to evaluate the effectiveness of an A/S based ointment for humans on second intention wound healing in the dog, as compared to wound flushing with Lactated Ringer’s solution (LRS). Ten mixed breed dogs, aged 2 to 5 y, were used. One 2.5 × 2.5 cm full-thickness skin defect was created on the lateral aspect of each arm for subjective evaluation, laser-Doppler flowmetry (LDF), and planimetry. Additionally, 3 matching 2 × 2 cm wounds were created on opposite sides of the dorsal midline for histologic evaluation. Wounds were treated once daily with the A/S based ointment on the right side and by flushing with LRS on the left until healed (about 20 d). During the healing process, tissue perfusion (mean LDF value) was found to be significantly higher on the side treated with the A/S based ointment compared with the LRS-treated side. Histologically, angiogenesis (on days 4 and 11), collagen production score (on days 4, 11, and 20), and epithelial thickness score (on day 11) were significantly higher in the wounds treated with the A/S based ointment. Wound size, as evaluated by planimetry, decreased significantly from day 0 to day 20 on both sides, but no significant differences were found between the A/S based ointment and LRS-treated wounds

Locally Injected Autologous Platelet-Rich Plasma Improves Cutaneous Wound Healing in Cats

Cutaneous defects in cats are commonly encountered in clinical practice, and healing can be accomplished by first or second intention. Platelet-rich plasma (PRP) is characterized by a plasma concentration containing a large number of platelets in a small volume of plasma. The objective of the present study was to record the efficacy of PRP infiltration in open wounds in laboratory cats. Six wounds were created in the dorsal midline of eight laboratory cats, with the wounds of one side designated as the PRP group and the wounds of the other side as the control group. Wound healing was evaluated by daily clinical examination, planimetry, laser Doppler flowmetry, and histologic examination on days 0, 7, 14, and 25, and by measurement of metalloproteinases (MMPs)-2 and -9 and tissue inhibitor metalloproteinase (TIMP)-1 on days 0, 14, and 25. Based on the results of the present study, the mean time for full coverage with granulation tissue was shorter in the PRP group, the mean contraction and total wound healing percentage were increased compared to the control group, and finally, the perfusion measured with laser Doppler flowmetry was higher in the PRP group during all examination days. In conclusion, this is the first study focusing on the topical application of PRP in the treatment of open wounds in laboratory cats, and our results are encouraging&mdash;showing a more rapid healing in the PRP group