7 research outputs found
Proteomic analysis of amniotic fluid in pregnancies with Down syndrome
Proteomic analysis is widely used for the detection of diagnostic markers. In the present study amniotic fluid supernatants?(AFS) from pregnancies with Down syndrome?(DS) fetuses and from chromosomally normal fetuses in the 17th?week of gestation were analyzed by 2-DE. Gel comparison revealed significant differences in the two groups. Spots with different expression levels were excised and proteins were identified by MALDI-MS and nano-ESI-MS/MS. Splicing factor arginine/serine-rich?4 (SFRS4; Q08170) was present only in AFS from DS fetuses and completely absent in the control group. Quantitative differences were detected for alpha-1-microglobulin (AMBP; P02760), collagen alpha?1?(I) chain (CO1A1; P02452), collagen alpha?1?(III) chain (CO3A1; P02461), collagen alpha?1?(V) chain?d (CO5A1; P20908), and basement membrane-specific heparin sulfate proteoglycan core protein (PGBM; P98160). These proteins were increased in cases with DS, whereas protein?IBP-1 (P08833) was decreased by 40% compared with chromosomally normal fetuses. Four proteins, CO1A1, CO3A1, CO5A1, and PGBM, appeared as fragments. As differentially expressed proteins were present in all pregnancies with DS tested, they may represent useful potential markers for prenatal diagnosis. However, for protein biomarkers to be of any clinical utility, systematic analysis of the maternal serum should be conducted
Proteomic analysis of amniotic fluid in pregnancies with Down syndrome
Proteomic analysis is widely used for the detection of diagnostic
markers. in the present study amniotic fluid supernatants (AFS) from
pregnancies with Down syndrome (DS) fetuses and from chromosomally
normal fetuses in the 17th week of gestation were analyzed by 2-DE. Gel
comparison revealed significant differences in the two groups. Spots
with different expression levels were excised and proteins were
identified by MALDI-MS and nano-ESI-MS/MS. Splicing factor
arginine/serine-rich 4 (SFRS4; Q08170) was present only in AFS from DS
fetuses and completely absent in the control group. Quantitative
differences were detected for alpha-1-microglobulin (AMBP; P02760),
collagen alpha 1 (1) chain (CO1A1; P02452), collagen alpha 1 (III) chain
(CO3A1; P02461), collagen alpha 1 (V) chain d (CO5A1; P20908), and
basement membrane-specific heparin sulfate proteoglycan core protein
(PGBM; P98160). These proteins were increased in cases with DS, whereas
protein IBP-1 (P08833) was decreased by 40% compared with chromosomally
normal fetuses. Four proteins, CO1A1, CO3A1, CO5A1, and PGBM, appeared
as fragments. As differentially expressed proteins were present in all
pregnancies with DS tested, they may represent useful potential markers
for prenatal diagnosis. However, for protein biomarkers to be of any
clinical utility, systematic analysis of the maternal serum should be
conducted
Vascular endothelial growth factor protein expression in a renal ablation rabbit model under prolonged warm and cold ischemia
Background/Aims: To establish a potential correlation between renal and
systemic production of vascular endothelial growth factor (VEGF) protein
after prolonged ischemia in a renal ablation model under normothermic
and hypothermic conditions. Methods: 38 uninephrectomized New Zealand
rabbits were divided into 5 groups. The rabbits of each group underwent
partial nephrectomy under 90 and 60 min of warm and 90 and 120 min of
cold ischemia, except for the sham group (S), which served as control.
Serum creatinine (SCr) and blood-urea-nitrogen (BUN) levels were
assessed. On the 15th postoperative day (POD), the animals were
euthanized and the remaining kidneys were evaluated. VEGF
immunohistochemistry and serum Western blot analysis were performed.
Results: In comparison to the control group, groups 60W, 90C and 120C
showed 1.6-, 1.14- and 1.75-fold decreases, respectively, while the
production of VEGF was significantly declined by 7.4-fold in group 90W
(p < 0.05). Immunohistochemistry revealed prominent VEGF staining in the
above-mentioned three groups, while in group 90W staining was negative.
Serum biochemistry and microscopic evaluation verified the same
differentiation. Conclusion: Renal and serum VEGF seem to have an
analogous expression under conditions of prolonged ischemia. VEGF is
overexpressed in hypothermic conditions compared to warm ischemia
exceeding 60 min. Hypothermia can be more advantageous in a procedure
applying prolonged ischemia. Copyright (C) 2007 S. Karger AG, Basel