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Distinct organization of the candidate tumor suppressor gene RFP2 in human and mouse: multiple mRNA isoforms in both species- and human-specific antisense transcript RFP2OS
In the present study, we describe the human and mouse
RFP2 gene structure, multiple
RFP2 mRNA isoforms in the two species that have different 5′ UTRs and a human-specific antisense transcript
RFP2OS. Since the human
RFP2 5′ UTR is not conserved in mouse, these findings might indicate a different regulation of
RFP2 in the two species. The predicted human and mouse RFP2 proteins are shown to contain a tripartite RING finger-B-box-coiled-coil domain (RBCC), also known as a TRIM domain, and therefore belong to a subgroup of RING finger proteins that are often involved in developmental and tumorigenic processes. Because homozygous deletions of chromosomal region 13q14.3 are found in a number of malignancies, including chronic lymphocytic leukemia (CLL) and multiple myeloma (MM), we suggest that RFP2 might be involved in tumor development. This study provides necessary information for evaluation of the role of
RFP2 in malignant transformation and other biological processes
A cosmid and cDNA fine physical map of a human chromosome 13q14 region frequently lost in B-cell chronic lymphocytic leukemia and identification of a new putative tumor suppressor gene, Leu5
B-cell chronic lymphocytic leukemia (B-CLL) is a human hematological neoplastic disease often associated with the toss of a chromosome 13 region between RB1 gene and locus D13S25, A new tumor suppressor gene (TSG) may be located in the region. A cosmid contig has been constructed between the Loci D13S1168 (W19598) and D13S25 (H2-42), which corresponds to the minimal region shared by B-CLL associated deletions. The contig includes more than 200 LANL and ICRF cosmid clones covering 620 kb, Three cDNAs likely corresponding to three different genes hare been found in the minimally deleted region, sequenced and mapped against the contigged cosmids, cDNA clone 10k4 as well as a chimeric clone 13g3, codes for a zinc-finger domain of the RING type and shares homology to some known genes involved in tumorigenesis (RET finger protein, BRCA1) and embryogenesis (MID1). We have termed the gene corresponding to 10k4/13g3 clones LEU5, This is the first gene with homology to known TSGs which has been found in the region of B-CLL rearrangements. (C) 1998 Federation of European Biochemical Societies