Epidemiology of plasmid-mediated quinolone resistance in salmonella enterica serovar typhimurium isolates from food-producing animals in Japan

A total of 225 isolates of Salmonella enterica serovar Typhimurium from food-producing animals collected between 2003 and 2007 were examined for the prevalence of plasmid-mediated quinolone resistance (PMQR) determinants, namely qnrA, qnrB, qnrC, qnrD, qnrS, qepA and aac(6')Ib-cr, in Japan. Two isolates (0.8%) of S. Typhimurium DT104 from different dairy cows on a single farm in 2006 and 2007 were found to have qnrS1 on a plasmid of approximately 9.6-kbp. None of the S. Typhimurium isolates had qnrA, qnrB, qnrC, qnrD, qepA and acc(6')-Ib-cr. Currently in Japan, the prevalence of the PMQR genes among S. Typhimurium isolates from food animals may remain low or restricted. The PFGE profile of two S. Typhimurium DT104 isolates without qnrS1 on the farm in 2005 had an identical PFGE profile to those of two S. Typhimurium DT104 isolates with qnrS1. The PFGE analysis suggested that the already existing S. Typhimurium DT104 on the farm fortuitously acquired the qnrS1 plasmid

Urinary Retention as an Initial Symptom of Acute Meningo-Encephalo-Radiculitis in Epstein-Barr Virus Infection

A 48-year-old man presented with urinary retention followed by disturbance of consciousness, areflexia, ophthalmoplegia, muscle weakness, and atrophy. Epstein-Barr virus DNA by PCR was positive in his cerebrospinal fluid. The cerebrospinal fluid revealed elevated myelin basic protein and an oligoclonal band. Magnetic resonance imaging showed high signal intensity in the pons, basal ganglia, and cerebral white matter on T2-weighted imaging and fluid-attenuated inversion recovery imaging. His consciousness, ophthalmoplegia, and muscle weakness almost full recovered. In this patient, the inflammation is thought to have begun as sacral radiculitis, and then extended to encephalitis, and brachial and lumbar radiculoneuritis

Chemical Characterization of a Volatile Dubnium Compound, DbOCl3

The formation and the chemical characterization of single atoms of dubnium (Db, element 105), in the form of its volatile oxychloride, was investigated using the on-line gas phase chromatography technique, in the temperature range 350–600 °C. Under the exactly same chemical conditions, comparative studies with the lighter homologues of Group 5 in the Periodic Table clearly indicate the volatility sequence being NbOCl3 > TaOCl3 ≥ DbOCl3. From the obtained experimental results, thermochemical data for DbOCl3 were derived. The present study delivers reliable experimental information for theoretical calculations on chemical properties of transactinides

Phylogenetic groups and cephalosporin resistance genes of Escherichia coli from diseased food-producing animals in Japan

A total of 318 Escherichia coli isolates obtained from different food-producing animals affected with colibacillosis between 2001 and 2006 were subjected to phylogenetic analysis: 72 bovine isolates, 89 poultry isolates and 157 porcine isolates. Overall, the phylogenetic group A was predominant in isolates from cattle (36/72, 50%) and pigs (101/157, 64.3%) whereas groups A (44/89, 49.4%) and D (40/89, 44.9%) were predominant in isolates from poultry. In addition, group B2 was not found among diseased food-producing animals except for a poultry isolate. Thus, the phylogenetic group distribution of E. coli from diseased animals was different by animal species. Among the 318 isolates, cefazolin resistance (minimum inhibitory concentrations: ≥32 μg/ml) was found in six bovine isolates, 29 poultry isolates and three porcine isolates. Of them, 11 isolates (nine from poultry and two from cattle) produced extended spectrum β-lactamase (ESBL). The two bovine isolates produced blaCTX-M-2, while the nine poultry isolates produced blaCTX-M-25 (4), blaSHV-2 (3), blaCTX-M-15 (1) and blaCTX-M-2 (1). Thus, our results showed that several types of ESBL were identified and three types of β-lactamase (SHV-2, CTX-M-25 and CTX-M-15) were observed for the first time in E. coli from diseased animals in Japan

DOCK2 is involved in the host genetics and biology of severe COVID-19

「コロナ制圧タスクフォース」COVID-19疾患感受性遺伝子DOCK2の重症化機序を解明 --アジア最大のバイオレポジトリーでCOVID-19の治療標的を発見--. 京都大学プレスリリース. 2022-08-10.Identifying the host genetic factors underlying severe COVID-19 is an emerging challenge. Here we conducted a genome-wide association study (GWAS) involving 2, 393 cases of COVID-19 in a cohort of Japanese individuals collected during the initial waves of the pandemic, with 3, 289 unaffected controls. We identified a variant on chromosome 5 at 5q35 (rs60200309-A), close to the dedicator of cytokinesis 2 gene (DOCK2), which was associated with severe COVID-19 in patients less than 65 years of age. This risk allele was prevalent in East Asian individuals but rare in Europeans, highlighting the value of genome-wide association studies in non-European populations. RNA-sequencing analysis of 473 bulk peripheral blood samples identified decreased expression of DOCK2 associated with the risk allele in these younger patients. DOCK2 expression was suppressed in patients with severe cases of COVID-19. Single-cell RNA-sequencing analysis (n = 61 individuals) identified cell-type-specific downregulation of DOCK2 and a COVID-19-specific decreasing effect of the risk allele on DOCK2 expression in non-classical monocytes. Immunohistochemistry of lung specimens from patients with severe COVID-19 pneumonia showed suppressed DOCK2 expression. Moreover, inhibition of DOCK2 function with CPYPP increased the severity of pneumonia in a Syrian hamster model of SARS-CoV-2 infection, characterized by weight loss, lung oedema, enhanced viral loads, impaired macrophage recruitment and dysregulated type I interferon responses. We conclude that DOCK2 has an important role in the host immune response to SARS-CoV-2 infection and the development of severe COVID-19, and could be further explored as a potential biomarker and/or therapeutic target

“Origins of Confucian capitalism in Japan: Ideology, ethics and policy in the thought of Shibusawa Eiichi”

Today, Japan is recognized as one of the industrialized countries in the world, especially in the realm of economy. However, the history of Japanese modernization is relatively short in comparison with other developed countries. It was the latter half of nineteenth century when the Meiji Restoration has occurred, a political and social process in which Tokugawa shogunate system had collapsed and the Edo period had come to an end of its 265 years of rule. Centralized government, Japanese capitalism have begun to be formulated at that moment. In a mere 150 years since then, Japan has already experienced a number of drastic changes up to the present day. Speaking of Japanese civilization, the practical progressesin technology tend to be focused, whereas there was a remarkable transition in relation to ideology, ethics which would reflect the confusions and conflicts that people experienced through the societal and economic changes. One would say that there are few countries who have accomplished a modernization in such a short period. Japan has experienced so many changes that some people would not even think that the civilizations we take for granted today such as capitalism, democracy, industrialized technology have appeared quite recently in the history. Furthermore, the anxiety that people had under the turbulent society in the late Edo period, which was just in front of the Meiji Restoration show remarkable similarity to what we assume to be typical problems of present age as well. Certainly, our predecessors at that age would not anticipate the tragic World War Ⅱ and subsequent occupation by the United States which would also leave the deep influence to Japan to this date, however, we could still see that the transition that Japan has experienced before and after the Meiji Restoration would continuously form the basis of today’s Japanese economic society. Therefore, I would like to suggest that it is beneficial to reinterpret the transition of Japanese society in this era through the eye of iconic person, Shibusawa Eiichi, would bring about not only a historical lesson but also a clue to construct a proposal for the present problems that we are facing

Individual Differences on the McGurk Effect: An Examination with the Autism Trait and Schizotypal Personality

The McGurk effect is a perceptual phenomenon that demonstrates interaction between hearing and vision in speech perception. This effect may be experienced when a visual of the production of a phoneme is dubbed with a sound recording of a different phoneme being spoken wherein the perceived phoneme is often the third intermediate phoneme. In the present study we examined the potential individual differences in the McGurk effect among 51 healthy students. The results suggested that people with higher scores for schizophrenic or autistic traits, respectively, might report less /ka/ responses (visually captured responses) but more /ta/ responses (vision-audio mixed responses) in the McGurk condition (visually /pa/ but auditor/ka/). This indicates that such people might show a preference for auditory information over visual information. Both schizophrenia and autism might have deficits in social functioning. If an individual has a poor level of interpersonal skills this would reflect in the result since he/she might not be able to read others' lips automatically

Carbohydrate intake is associated with time spent in the euglycemic range in patients with type 1 diabetes.

[Aims/Introduction]Greater glycemic variability and lack of predictability are important issues for patients with type 1 diabetes. Dietary factors are one of the contributors to this variability, but how closely diet is linked to glycemic fluctuation on a daily basis has not been investigated. We examined the association between carbohydrate intake and glycemic excursion in outpatients. [Materials and Methods]A total of 33 patients with type 1 diabetes were included in the analyses (age 44.5 ± 14.7 years, diabetes duration 15.1 ± 8.3 years, 64% female, 30% using insulin pump, glycated hemoglobin 8.1 ± 1.3%). Time spent in euglycemia (70–180 mg/dL), hyperglycemia (>180 mg/dL) and hypoglycemia (<70 mg/dL) of consecutive 48-h periods of continuous glucose monitoring data were collected together with simultaneous records of dietary intake, insulin dose and physical activity. Correlation analyses and multiple regression analyses were used to evaluate the contribution of carbohydrate intake to time spent in the target glycemic range. [Results]In multiple regression analyses, carbohydrate intake (β = 0.53, P = 0.001), basal insulin dose per kg per day (β = −0.31, P = 0.034) and diabetes duration (β = 0.30, P = 0.042) were independent predictors of time spent in euglycemia. Carbohydrate intake (β = −0.51, P = 0.001) and insulin pump use (β = −0.34, P = 0.024) were independent predictors of time spent in hyperglycemia. Insulin pump use (β = 0.52, P < 0.001) and bolus insulin dose per kg per day (β = 0.46, P = 0.001) were independent predictors of time spent in hypoglycemia. [Conclusions]Carbohydrate intake is associated with time spent in euglycemia in patients with type 1 diabetes