Extensive Structural Variations Between Mitochondrial Genomes of CMS and Normal Peppers (Capsicum Annuum L.) Revealed by Complete Nucleotide Sequencing

Cytoplasmic male sterility (CMS) is an inability to produce functional pollen that is caused by mutation of the mitochondrial genome. Comparative analyses of mitochondrial genomes of lines with and without CMS in several species have revealed structural differences between genomes, including extensive rearrangements caused by recombination. However, the mitochondrial genome structure and the DNA rearrangements that may be related to CMS have not been characterized in Capsicum spp. Results: We obtained the complete mitochondrial genome sequences of the pepper CMS line FS4401 (507,452 bp) and the fertile line Jeju (511,530 bp). Comparative analysis between mitochondrial genomes of peppers and tobacco that are included in Solanaceae revealed extensive DNA rearrangements and poor conservation in non-coding DNA. In comparison between pepper lines, FS4401 and Jeju mitochondrial DNAs contained the same complement of protein coding genes except for one additional copy of an atp6 gene (psi atp6-2) in FS4401. In terms of genome structure, we found eighteen syntenic blocks in the two mitochondrial genomes, which have been rearranged in each genome. By contrast, sequences between syntenic blocks, which were specific to each line, accounted for 30,380 and 17,847 bp in FS4401 and Jeju, respectively. The previously-reported CMS candidate genes, orf507 and psi atp6-2, were located on the edges of the largest sequence segments that were specific to FS4401. In this region, large number of small sequence segments which were absent or found on different locations in Jeju mitochondrial genome were combined together. The incorporation of repeats and overlapping of connected sequence segments by a few nucleotides implied that extensive rearrangements by homologous recombination might be involved in evolution of this region. Further analysis using mtDNA pairs from other plant species revealed common features of DNA regions around CMS-associated genes. Conclusions: Although large portion of sequence context was shared by mitochondrial genomes of CMS and male-fertile pepper lines, extensive genome rearrangements were detected. CMS candidate genes located on the edges of highly-rearranged CMS-specific DNA regions and near to repeat sequences. These characteristics were detected among CMS-associated genes in other species, implying a common mechanism might be involved in the evolution of CMS-associated genes.Golden Seed ProjectMinistry of Agriculture, Food and Rural Affairs (MAFRA)Ministry of Oceans and Fisheries (MOF)Rural Development Administration (RDA)Korea Forest Service (KFS)Vegetable Breeding Research Center through the R&D Convergence Center Support Program, Ministry of Agriculture, Food and Rural Affairs (MAFRA) Republic of Korea 710001-07Molecular Bioscience

Teaching Vocabulary at the Upper Primary Levels with the Vocabulary Self-Selection Strategy

In this paper, the authors describe a classroom inquiry by a newly graduated English language teacher on the explicit teaching of vocabulary with one class of 40 pupils at the upper primary levels through the Vocabulary Self-Collection Strategy (VSS) (Haggard, 1982, 1986; Ruddell, 2008a). Findings from this inquiry reveal that the VSS strategy designed primarily for the development of vocabulary in academic or content areas in learners was also suitable for the learning of thematically-related words in fiction texts as well. In addition, it is argued that since the strategy was new to this class of pupils, prioritising word study skills over the identification of key words in a text is a useful primer to the use of the strategy. The article ends with the implications of the strategy implementation on the pupils, the teacher and her own classroom practice as an English language teacher

Electrochemical Investigation of High-Performance Dye-Sensitized Solar Cells Based on Molybdenum for Preparation of Counter Electrode

In order to improve the photocurrent conversion efficiency of dye-sensitized solar cells (DSSCs), we studied an alternative conductor for the counter electrode and focused on molybdenum (Mo) instead of conventional fluorine-doped tin oxide (FTO). Because Mo has a similar work function to FTO for band alignment, better formability of platinum (Pt), and a low electric resistance, using a counter electrode made of Mo instead of FTO lead to the enhancement of the catalytic reaction of the redox couple, reduce the interior resistance of the DSSCs, and prevent energy-barrier formation. Using electrical measurements under a 1-sun condition (100 mW/cm(2), AM 1.5), we determined that the fill factor (FF) and photocurrent conversion efficiency (eta) of DSSCs with a Mo electrode were respectively improved by 7.75% and 5.59% with respect to those of DSSCs with an FTO electrode. Moreover, we have investigated the origin of the improved performance through surface morphology analyses such as scanning electron microscopy and electrochemical analyses including cyclic voltammetry and impedance spectroscopy

From Values to Opinions: Predicting Human Behaviors and Stances Using Value-Injected Large Language Models

Being able to predict people's opinions on issues and behaviors in realistic scenarios can be helpful in various domains, such as politics and marketing. However, conducting large-scale surveys like the European Social Survey to solicit people's opinions on individual issues can incur prohibitive costs. Leveraging prior research showing influence of core human values on individual decisions and actions, we propose to use value-injected large language models (LLM) to predict opinions and behaviors. To this end, we present Value Injection Method (VIM), a collection of two methods -- argument generation and question answering -- designed to inject targeted value distributions into LLMs via fine-tuning. We then conduct a series of experiments on four tasks to test the effectiveness of VIM and the possibility of using value-injected LLMs to predict opinions and behaviors of people. We find that LLMs value-injected with variations of VIM substantially outperform the baselines. Also, the results suggest that opinions and behaviors can be better predicted using value-injected LLMs than the baseline approaches.Comment: EMNLP 2023 main paper accepte

Collegiate Student-Athletes’ Privacy Management Strategies and Their Impact on Twitter Usage Behaviors

This study examines collegiate student-athletes’ privacy management strategies and the impact on their Twitter usage behaviors from Communication Privacy Management Theory (CPM). A questionnaire was used to recruit student-athletes from a national sample of NCAA Division 1 universities in the United States. Three hierarchical regression analyses conclude that collegiate student-athletes’ privacy management strategies would affect their Twitter usage behaviors, such as frequency of checking Twitter, minutes spent on the platform and tweet content . This research extends CPM to the collegiate sports context. Implications are discussed