An Investigation into the Cytotoxic Effects of 13-Acetoxysarcocrassolide from the Soft Coral Sarcophyton crassocaule on Bladder Cancer Cells

Active compounds from natural products have been widely studied. The anti-tumor effects of 13-acetoxysarcocrassolide isolated from Formosan soft coral Sarcophyton crassocaule on bladder cancer cells were examined in this study. An MTT assay showed that 13-acetoxysarcocrassolide was cytotoxic to bladder female transitional cancer (BFTC) cells. We determined that the BFTC cells underwent cell death through apoptosis by flow cytometry. Due to the highly-migratory nature of the BFTC cells, the ability of 13-acetoxysarcocrassolide to stop their migration was assessed by a wound healing assay. To determine which proteins were affected in the BFTC cells upon treatment, a comparative proteomic analysis was performed. By LC-MS/MS analysis, we identified that 19 proteins were up-regulated and eight were down-regulated. Seven of the proteins were confirmed by western blotting analysis. This study reveals clues to the potential mechanism of the cytotoxic effects of 13-acetoxysarcocrassolide on BFTC cells. Moreover, it suggests that PPT1 and hnRNP F could be new biomarkers for bladder cancer. The results of this study are also helpful for the diagnosis, progression monitoring and therapeutic strategies of transitional cell tumors

Condition Assessment of Power Transformer Onload Tap Changers Using Wavelet Analysis and Self-Organizing Map: Field Evaluation

An onload tap changer (OLTC) is the most maintenance intensive subassembly on a power transformer. Vibration monitoring is an effective technique that can be used to assess the condition of an OLTC nonintrusively. The authors have developed a condition monitoring system for common types of OLTCs that enables the condition of tap changer contacts and associated drive system to be inferred from vibration signals. A number of prototype systems have been installed onto OLTCs in distribution zone substations for field trials. Particular emphasis has been given to the detection of faults in a particular type of older tap changer that had been prone to a range of faults associated with the switching contacts and drive mechanism. For this type of tap changer, it has been shown to be possible to determine not only that the tap changer is aging but also to identify the particular part that is degrading

Characterisation of Vibration Signals Using Continuous Wavelet Transform for Condition Assessment of On-Load Tap-Changers

The operation of an on-load tap-changer (OLTC) on a high-voltage power transformer produces a vibration signal that consists of a series of well-defined transients. Due to the harmonic and non-stationary nature of transient vibration signals, the traditional frequency and time–frequency techniques are not effective for characterising this type of vibration signal. The time domain features such as delays in time between transients, and the number and strengths of transients are more important for the assessment of the OLTC condition. This paper details the development of an OLTC vibration signal characterisation technique using the continuous wavelet transform (CWT). The CWT, due to its capability of multi-scale localisation in the time domain, is an effective tool in extracting the essential features from the original signals. These features are utilised to provide reliable indications of the health of the equipment. Signals from real plant are used to demonstrate the usefulness of the proposed technique

Design of a Subsequent Water Detection System Controlled by the Microcontroller

This article instructs the subsequent water detection system design and implementation of sanitary ware. This system used C8051f040 Microcontroller as the main control module and communication module which controlled the operation of the entire system，touch screen as a position machine，and made use of metal probing technique and weighing sensor technology to realize data test、collection、display、storage and export. At last，the experimental results showed that this system meet the expected requirement and can measure the subsequent water more accurately. DOI : http://dx.doi.org/10.11591/telkomnika.v12i1.4140