Characterisation of novel biomass degradation enzymes from the genome of <i>Cellulomonas fimi</i>

Recent analyses of genome sequences belonging to cellulolytic bacteria have revealed many genes potentially coding for cellulosic biomass degradation enzymes. Annotation of these genes however, is based on few biochemically characterised examples. Here we present a simple strategy based on BioBricks for the rapid screening of candidate genes expressed in Escherichia coli. As proof of principle we identified over 70 putative biomass degrading genes from bacterium Cellulomonas fimi, expressing a subset of these in BioBrick format. Six novel genes showed activity in E. coli. Four interesting enzymes were characterised further. α-l-arabinofuranosidase AfsB, β-xylosidases BxyF and BxyH and multi-functional β-cellobiosidase/xylosidase XynF were partially purified to determine their optimum pH, temperature and kinetic parameters. One of these enzymes, BxyH, was unexpectedly found to be highly active at strong alkaline pH and at temperatures as high as 100 °C. This report demonstrates a simple method of quickly screening and characterising putative genes as BioBricks

Why P/OF should look for evidences of over-dense structures in solar flare hard X-ray sources

White-light and hard X-ray (HXR) observations of two white-light flares (WLFs) show that if the radiative losses in the optical continuum are powered by fast electrons directly heating the WLF source, then the column density constraints imposed by the finite range of the electrons requires that the WLF consist of an over-dense region in the chromosphere, with density exceeding 10 to the 14th power/cu cm. Thus, we recommend that P/OF search for evidences of over-dense structures in HXR images obtained simultaneously with optical observations of flares

Shot Noise in Anyonic Mach-Zehnder Interferometer

We show how shot noise in an electronic Mach-Zehnder interferometer in the fractional quantum Hall regime probes the charge and statistics of quantum Hall quasiparticles. The dependence of the noise on the magnetic flux through the interferometer allows for a simple way to distinguish Abelian from non-Abelian quasiparticle statistics. In the Abelian case, the Fano factor (in units of the electron charge) is always lower than unity. In the non-Abelian case, the maximal Fano factor as a function of the magnetic flux exceeds one.Comment: references adde

Gate-Controlled Electron Spin Resonance in a GaAs/AlGaAs Heterostructure

The electron spin resonance (ESR) of two-dimensional electrons is investigated in a gated GaAs/AlGaAs heterostructure. We found that the ESR resonance frequency can be turned by means of a gate voltage. The front and back gates of the heterostructure produce opposite g-factor shift, suggesting that electron g-factor is being electrostatically controlled by shifting the equilibrium position of the electron wave function from one epitaxial layer to another with different g-factors

Spin Relaxation in a Quantum Dot due to Nyquist Noise

We calculate electron and nuclear spin relaxation rates in a quantum dot due to the combined action of Nyquist noise and electron-nuclei hyperfine or spin-orbit interactions. The relaxation rate is linear in the resistance of the gate circuit and, in the case of spin-orbit interaction, it depends essentially on the orientations of both the static magnetic field and the fluctuating electric field, as well as on the ratio between Rashba and Dresselhaus interaction constants. We provide numerical estimates of the relaxation rate for typical system parameters, compare our results with other, previously discussed mechanisms, and show that the Nyquist mechanism can have an appreciable effect for experimentally relevant systems.Comment: v2: New discussion of arbitrary gate setups (1 new figure), more Comments on experiments; 6 pages, 4 figure

Weak Localization Thickness Measurements of Si:P Delta-Layers

We report on our results for the characterization of Si:P delta-layers grown by low temperature molecular beam epitaxy. Our data shows that the effective thickness of a delta-layer can be obtained through a weak localization analysis of electrical transport measurements performed in perpendicular and parallel magnetic fields. An estimate of the diffusivity of phosphorous in silicon is obtained by applying this method to several samples annealed at 850 Celsius for intervals of zero to 15 minutes. With further refinements, this may prove to be the most precise method of measuring delta-layer widths developed to date, including that of Secondary Ion Mass Spectrometry analysis

Viscous Effects on Impact Probes in a Subsonic Rarified Gas Flow

The problem of time response of pressure gaging systems used with low density flows has been discussed in Ref ]4, and the effect of outgassing on pressure magnitudes was indicated in the same reference. Briefly, gases or vapors adhering to the internal walls of the pressure gaging system behave like gas sources and produce a pressure rise in the gage system which has no relation to the external flow. The effect can also occur in the reverse direction, with "in-gassing" or the action of an effective sink in the gage system as gases entering through the probe orifice are adsorbed to the walls. For given surface conditions, the magnitude of the pressure error to be expected due to out-gassing depends on the dimensions of the probe system. In the present tests, it was desirable to use the smallest possible probe to yield the lowest possible Reynolds number. The lower limit on size was fixed by outgassing effects, evaluated by the following procedure. 2.0 PROCEDURE The pumping system was adjusted to give a pressure, measured at the reservoir, of 9 microns Hg with no flow into the wind tunnel. The upstream metering valve was then opened and the air flow rate adjusted to give a pressure of 100 microns in the reservoir under steady flow conditions, The pressure read by one of the impact tubes, inserted into the flow, was measured. The upstream metering valve was then closed rapid)y, and the reservoir pressure, p0, and the impact probe pressure, Pj, were measured simultaneously at definite time intervals. Another probe was inserted into the flow and the procedure repeated, until the pressure-time data had been obtained for each probe investigated. The results for a series of tests Involving probe Nos.l, 2 and 3 are shown on HYD 2616 300 RESULTS From HYD 2616, It is clear that the smallest probe (No.3) requires the longest time to reach equilibrium. After a sufficient time has elapsed (about 180 seconds), this probe read the same, within the accuracy of measurement, as the other two, When a similar experiment was performed utilizing a probe which was one-half the size of probe No.2, it indicated a pressure, after 180 seconds, which was almost 10 microns Hg higher than the other probes. Accordingly, only probe Nos. 1, 2, 3 and 4 were employed in the experiments, and a time of at least 180 seconds was allowed to elapse between a change of setting and the reading of the instruments

Is there a renormalization of the 1D conductance in Luttinger Liquid model?

Properties of 1D transport strongly depend on the proper choice of boundary conditions. It has been frequently stated that the Luttinger Liquid (LL) conductance is renormalized by the interaction as $g \frac{e^2} {h}$. To contest this result I develop a model of 1D LL wire with the interaction switching off at the infinities. Its solution shows that there is no renormalization of the universal conductance while the electrons have a free behavior in the source and drain reservoirs.Comment: 5 pages, RevTex 2.0, attempted repair of tex error

Transport in an inhomogeneous interacting one--dimensional system

Transport through a one--dimensional wire of interacting electrons connected to semi--infinite leads is investigated using a bosonization approach. An incident electron is transmitted as a sequence of partial charges. The dc conductance is found to be entirely determined by the properties of the leads. The dynamic nonlocal conductivity is rigorously expressed in terms of the transmission. For abrupt variations of the interaction parameters at the junctions the central wire acts as a Fabry--Perot resonator. When one of the connected wires has a tendency towards superconducting order, partial Andreev reflection of an incident electron occurs.Comment: 11 pages, RevTeX 3.0, 1 postscript figure, everything in a uuencoded fil