Chronic obstructive pulmonology disease in gastro-intestinal pathology patients (literature review)

The objective of this paper is to analyze recent studies on correlation between stomach pathology and respiratory apparatus. It presents gastro-intestinal pathology impact on characteristic and chronic obstructive pulmonology disease cource weight and describes some digestive apparatus pathology conerned with para-endocrine system disfunction.Представлен анализ литературных данных о взаимосвязи патологии органов пищеварения и дыхания. Приведены данные о влиянии гастроинтестинальной патологии, в том числе дисфункции энтероэндокринной системы, на особенности и тяжесть течения хронической обструктивной болезни легких

КЛИНИКО-МОРФОЛОГИЧЕСКАЯ ХАРАКТЕРИСТИКА ЭНТЕРОЭНДОКРИННОЙ СИСТЕМЫ У БОЛЬНЫХ ХРОНИЧЕСКИМ ОБСТРУКТИВНЫМ БРОНХИТОМ

Introduction. Comorbidity greatly contributes to progression and severity of chronic obstructive pulmonary disease (COPD). The aim of this study was to describe the nature and severity of gastrointestinal paraendocrine disorders in patients with COPD.Methods. This cross-sectional open randomized controlled study included 84 patients with COPD. We measured blood serotonin concentration and performed immunohistochemical investigation of gastrointestinal APUD cells.Results. Our results suggested an effect of gastrointestinal disease on the severity of COPD. Hyperserotoninemia and structural and functional disorders in enteroendocrine system were noted even in patients with mild COPD.Conclusion. The severity of bronchial obstruction could correlate with functional activity of serotonin-producing enteroendocrine cells.В прогрессировании и тяжести течения хронической обструктивной болезни легких (ХОБЛ) придается значение сопутствующей патологии. С целью установления характера и тяжести поражения параэндокринной системы гастроинтестинальной зоны у пациентов с различными стадиями ХОБЛ проведено одномоментное исследование, в которое были включены пациенты (n = 84) с ХОБЛ. Выполнено исследование уровня серотонина крови, иммуногистохимическое исследование апудоцитов гастроинтестинальной зоны. Получены данные о влиянии гастроинтестинальной патологии на тяжесть течения ХОБЛ. Установлено, что тяжесть бронхообструктивного синдрома коррелирует с функциональной активностью серотонинпродуцирующих энтероэндокриноцитов

The comparative responsiveness of Hospital Universitario Princesa Index and other composite indices for assessing rheumatoid arthritis activity

Objective To evaluate the responsiveness in terms of correlation of the Hospital Universitario La Princesa Index (HUPI) comparatively to the traditional composite indices used to assess disease activity in rheumatoid arthritis (RA), and to compare the performance of HUPI-based response criteria with that of the EULAR response criteria. Methods Secondary data analysis from the following studies: ACT-RAY (clinical trial), PROAR (early RA cohort) and EMECAR (pre-biologic era long term RA cohort). Responsiveness was evaluated by: 1) comparing change from baseline (Delta) of HUPI with Delta in other scores by calculating correlation coefficients; 2) calculating standardised effect sizes. The accuracy of response by HUPI and by EULAR criteria was analyzed using linear regressions in which the dependent variable was change in global assessment by physician (Delta GDA-Phy). Results Delta HUPI correlation with change in all other indices ranged from 0.387 to 0.791); HUPI's standardized effect size was larger than those from the other indices in each database used. In ACT-RAY, depending on visit, between 65 and 80% of patients were equally classified by HUPI and EULAR response criteria. However, HUPI criteria were slightly more stringent, with higher percentage of patients classified as non-responder, especially at early visits. HUPI response criteria showed a slightly higher accuracy than EULAR response criteria when using Delta GDA-Phy as gold standard. Conclusion HUPI shows good responsiveness in terms of correlation in each studied scenario (clinical trial, early RA cohort, and established RA cohort). Response criteria by HUPI seem more stringent than EULAR''s

The problems of protecting the rights of heirs of intellectual rights in court

This article is devoted to the analysis of problems arising in the process of judicial protection of intellectual property rights of heirs. The article, on the basis of doctrinal and legal sources, confirms the absence of the right for the heirs of the author to demand moral compensation in case there is a limit to the work of the author or the artisans, which is one of the practices to protect the judiciary. In addition, the authors determined that in the presence of a judicial dispute concerning the privacy rights of a particular individual, this fact could be verified. by any evidence, including an alienation agreement, and the presence of the corresponding state registration. Also, during the preparation of this article, the authors have made a decision that status of the heir of exclusive rights is determined by the conceptual difference in the rights included in the estate, that is, property rights or non-property, and also directly depends on the limits of rights and duties of the testator, which determines the methods used judicial protection. At the same time, the study made it possible for us to realize that, despite the presence in the Republic of Kazakhstan of a number of legislative provisions regulating the issue the scope on the rights of each holder of exclusive rights to judicial protection, it remains an urgent problem. Key words: protection, court, author, intellectual property, exclusive right

Congenital disorder of glycosilation PMM2-CDG

Congenital glycosylation disorders represent a group of genetically determined diseases which violate the synthesis and addition of glycans to glycoproteins and glycolipids, and also the synthesis of glycosylphosphatidyl inositol. The most common defects are the defects of protein N-glycosylation. Jaken syndrome, a congenital disorder of PMM2-CDG glycosylation, is the most commonly diagnosed type (about 800 cases worldwide). However, there are only a few descriptions of clinical cases in the Russian literature. The article presents a clinical observation of a child with this type of congenital glycosylation disorder due to a defect in phosphomannomtase 2 (PMM2 gene). The diagnose was based on the combination of clinical, laboratory and instrumental data: a characteristic phenotype, hyperinsulinism, delayed physical and psychomotor development, neurological manifestations, coagulopathy, liver damage, exudative enteropathy, abnormal forms of transferrin, PMM2 gene mutations associated with Jaken’s syndrome. For the first time the authors described positive clinical and laboratory dynamics due to the inclusion of D-mannose to the therapy for this type of congenital glycosylation disorder

Studies of biological properties of continuous suspension ВНК-21/SUSP/ARRIAH cell line

The results of the studies of cytomorphological, karyological, cultural properties of continuous suspension ВНК-21/SUSP/ARRIAH subline of newborn Syrian hamster kidney cells intended for foot-and-mouth disease, rabies, bovine parainfluenza-3, Aujeszky’s disease virus reproduction, as well as for production of diagnostic veterinary biologicals are presented. When cultured in suspension, BHK-21/SUSP/ARRIAH cell subline undergoes selection towards hypoploidy: modal class is represented by cells with 41 chromosomes (32–40% of cells); the share of cells containing 40–42 chromosomes is 78–80%; the share of polyploids averages around 1%; the range of variation in the number of chromosomes is from 36 to 54. BHK-21/SUSP/ARRIAH cell subline cultured in suspension with cell seeding concentration of 0.6–0.8 million cells/cm3 demonstrates growth rate of 6.67–11.00 and 96–99% cell viability. After 48 hours, G1-phase (diploid-2n) cells prevail in the cell population of the new subline (30.0–75.0% of cells); cells that undergo preparation for mitosis (S-phase) and mitosis (G2+M-phase) account for 3.0 to 20.0% of the entire population; the number of meganucleated and multinucleated cells (>4n) at the beginning and at the end of the logarithmic phase increases to 2%. BHK-21/SUSP/ARRIAH cells recover rapidly after cryopreservation and demonstrate 95–99% viability and growth rate of 3.36–5.88 at passages 1 to 3 and 6.85–10.95 at passages 4 to 12. Continuous suspension BHK-21/SUSP/ARRIAH cell line ensures virus accumulation at the following titres: FMD virus – 7.30– 8.00 lg TCID50/cm3, rabies virus – 7.25–8.00 lg CCID50/cm3, bovine parainflunza-3 virus – at least 6.00 lg TCID50/cm3, Aujeszky’s disease virus – 7.50–7.80 lg TCID50/cm3