Minimal information for studies of extracellular vesicles (MISEV2023): From basic to advanced approaches

Extracellular vesicles (EVs), through their complex cargo, can reflect the state of their cell of origin and change the functions and phenotypes of other cells. These features indicate strong biomarker and therapeutic potential and have generated broad interest, as evidenced by the steady year-on-year increase in the numbers of scientific publications about EVs. Important advances have been made in EV metrology and in understanding and applying EV biology. However, hurdles remain to realising the potential of EVs in domains ranging from basic biology to clinical applications due to challenges in EV nomenclature, separation from non-vesicular extracellular particles, characterisation and functional studies. To address the challenges and opportunities in this rapidly evolving field, the International Society for Extracellular Vesicles (ISEV) updates its 'Minimal Information for Studies of Extracellular Vesicles', which was first published in 2014 and then in 2018 as MISEV2014 and MISEV2018, respectively. The goal of the current document, MISEV2023, is to provide researchers with an updated snapshot of available approaches and their advantages and limitations for production, separation and characterisation of EVs from multiple sources, including cell culture, body fluids and solid tissues. In addition to presenting the latest state of the art in basic principles of EV research, this document also covers advanced techniques and approaches that are currently expanding the boundaries of the field. MISEV2023 also includes new sections on EV release and uptake and a brief discussion of in vivo approaches to study EVs. Compiling feedback from ISEV expert task forces and more than 1000 researchers, this document conveys the current state of EV research to facilitate robust scientific discoveries and move the field forward even more rapidly

The Status of Professional Nursing Roles in Nursing MSc Theses

Introduction: Nursing theses are among the most important sources to generate knowledge for nursing practice. Consideration of the five professional nursing roles is necessary for professional development of the field. This study aimed to assess the level of consideration of professional nursing roles in nursing MSc theses and how it has changed during 2002-14. Methods: This descriptive cross-sectional study reviewed nursing MSc theses written during 2002-14 in Mashhad School of Nursing and Midwifery. Theses’ titles were reviewed in terms of the five nursing roles including caregiving, educational, communicative, advocating and managerial roles. Data collection tool was a researcher-made checklist for identifying the themes of theses according to the five research topics. Results: A total of 160 theses had been written in this period. Of these, 37.5% were related to caregiving role, 18.75% to educational role, 1.8% to communicative role, 6.25% to advocating role and 5.7% were related to managerial role while 30% addressed issues other than these roles. During these years, nursing MSc theses have been focused more on the caregiving role and the communicative role has received the least attention. The communicative, managerial and educational roles have gradually received further attention in this period; however, the number of miscellaneous topics has gone up too. Conclusion: Given the importance of addressing the five nursing roles to contribute to the professional development of this field, it is necessary that future nursing theses should be more focused on and related to these roles

Multilevel drought hazard assessment under climate change scenarios in semi-arid regions - A case study of the Karkheh River Basin in Iran

Studies using Drought Hazard Indices (DHIs) have been performed at various scales, but few studies associated DHIs of different drought types with climate change scenarios. To highlight the regional differences in droughts at meteorological, hydrological, and agricultural levels, we utilized historic and future DHIs derived from the Standardized Precipitation Index (SPI), Standardized Runoff Index (SRI), and Standardized Soil Water Index (SSWI), respectively. To calculate SPI, SRI, and SSWI, we used a calibrated Soil and Water Assessment Tool (SWAT) for the Karkheh River Basin (KRB) in Iran. Five bias-corrected Global Circulation Models (GCMs) under two Intergovernmental Panel on Climate Change (IPCC) scenarios projected future climate. For each drought type, we aggregated drought severity and occurrence probability rate of each index into a unique DHI. Five historic droughts were identified with different characteristics in each type. Future projections indicated a higher probability of severe and extreme drought intensities for all three types. The duration and frequency of droughts were predicted to decrease in precipitation-based SPI. However, due to the impact of rising temperature, the duration and frequency of SRI and SSWI were predicted to intensify. The DHI maps of KRB illustrated the highest agricultural drought exposures. Our analyses provide a comprehensive way to monitor multilevel droughts complementing the existing approaches.ISSN:2073-444

Menstrual disorders and premenstrual symptoms in adolescents: prevalence and relationship to serum calcium and vitamin D concentrations

There have been several studies evaluating the association between vitamin and mineral status and menstrual disturbance. In the present study, we aimed to assess the relationship between the menstrual bleeding pattern and premenstrual syndrome (PMS) symptoms with serum 25-hydroxyvitamin D, and calcium levels in adolescent girls. A cross-sectional study was carried out in 897 high school girls from northeastern Iran. The prevalence of hypocalcaemia, normal serum calcium and hypercalcaemia was 27.1, 59.8 and 13.1%, respectively. The menstrual flow of participants differed significantly between the calcium status groups (p = .005). There was no significant association between the symptoms of PMS, as assessed by the questionnaire and serum vitamin D status, or serum calcium concentrations, apart from the irritability. There appears to be an association between serum calcium, menstrual blood loss and irritability in adolescent girls.Impact statement What is already known on this subject? Several studies have evaluated the association of vitamin and mineral status with menstrual disturbance, although these relationships are not consistent, specifically among calcium and vitamin D levels with a menstrual bleeding pattern. What do the results of this study add? In the present study, we investigated the correlation of menstrual bleeding patterns and PMS with calcium and vitamin D levels in a large population in adolescent girls. We found that the level of calcium was associated with the level of menstrual blood loss and irritability. However, no significant association was observed between the menstrual bleeding pattern or the PMS symptoms with a vitamin D status. What are the implications of these findings for future clinical practise/research? Further studies are required to assess the value of a calcium adequate intake or a calcium supplementation for the amelioration of PMS and a better understanding the role of calcium in PMS