5 research outputs found
Synthesis and characterization of Sn‑doped TiO2 flm for antibacterial applications
Simple sol–gel method has been exploited to deposit Sn-doped TiO2 thin flms on glass substrates. The resultant coatings
were characterized by X-ray difraction (XRD), UV–visible techniques (UV–Vis), Fourier transform infrared spectroscopy
(FTIR), and photoluminescence analysis (PL). The XRD pattern reveals an increase in crystallite size of the prepared samples
with the increasing doping concentration. A decrease in doping concentrating resulted in the decrease in bandgap values. The
diferent chemical bonds on these flms were identifed from their FTIR spectra. The photoluminescence analysis shows an
increase in the emission peak intensity with increasing dopant concentration, and this can be attributed to the efect created
due to surface states. The prepared samples were tested as antibacterial agent toward both Gram-positive and Gram-negative
bacteria like S.aureus (Staphylococcus aureus) and E.coli (Escherichia coli), respectively. The size of the inhibition zones
indicates that the sample shows maximum inhibitory property toward E.coli when compared to S.aureus
Plant Isoquinoline Alkaloid Berberine Exhibits Chromatin Remodeling by Modulation of Histone Deacetylase To Induce Growth Arrest and Apoptosis in the A549 Cell Line
Histone
deacetylases (HDACs) are a group of epigenetic enzymes
that control gene expression through their repressive influence on
histone deacetylation transcription. HDACs are probable therapeutic
targets for cancer treatment, spurring the progress of different types
of HDAC inhibitors. Further, natural-source-based derived bioactive
compounds possess HDAC inhibitor property. In this way, we hypothesized
that plant isoquinoline alkaloid berberine (BBR) could be a HDAC inhibitor
in the human lung cancer A549 cell line. BBR represses total HDAC
and also class I, II, and IV HDAC activity through hyperacetylation
of histones. Furthermore, BBR triggers positive regulation of the
sub-G<sub>0</sub>/G<sub>1</sub> cell cycle progression phase in A549
cells. Moreover, BBR-induced A549 cell growth arrest and morphological
changes were confirmed using different fluorescence-dye-based microscope
techniques. Additionally, BBR downregulates oncogenes (TNF-α,
COX-2, MMP-2, and MMP-9) and upregulates tumor suppressor genes (p21
and p53) mRNA and protein expressions. Besides, BBR actively regulates
Bcl-2/Bax family proteins and also triggered the caspase cascade apoptotic
pathway in A549 cells. Our finding suggests that BBR mediates epigenetic
reprogramming by HDAC inhibition, which may be the key mechanism for
its antineoplastic activity