32 research outputs found
A genome-wide association study explores the genetic determinism of host resistance to Salmonella pullorum infection in chickens
International audienceAbstractBackgroundSalmonella infection is a serious concern in poultry farming because of its impact on both economic loss and human health. Chicks aged 20Â days or less are extremely vulnerable to Salmonella pullorum (SP), which causes high mortality. Furthermore, an outbreak of SP infection can result in a considerable number of carriers that become potential transmitters, thus, threatening fellow chickens and offspring. In this study, we conducted a genome-wide association study (GWAS) to detect potential genomic loci and candidate genes associated with two disease-related traits: death and carrier state.MethodsIn total, 818 birds were phenotyped for death and carrier state traits through a SP challenge experiment, and genotyped by using a 600Â K high-density single nucleotide polymorphism (SNP) array. A GWAS using a single-marker linear mixed model was performed with the GEMMA software. RNA-sequencing on spleen samples was carried out for further identification of candidate genes.ResultsWe detected a region that was located between 33.48 and 34.03Â Mb on chicken chromosome 4 and was significantly associated with death, with the most significant SNP (rs314483802) accounting for 11.73% of the phenotypic variation. Two candidate genes, FBXW7 and LRBA, were identified as the most promising genes involved in resistance to SP. The expression levels of FBXW7 and LRBA were significantly downregulated after SP infection, which suggests that they may have a role in controlling SP infections. Two other significant loci and related genes (TRAF3 and gga-mir-489) were associated with carrier state, which indicates a different polygenic determinism compared with that of death. In addition, genomic inbreeding coefficients showed no correlation with resistance to SP within each breed in our study.ConclusionsThe results of this GWAS with a carefully organized Salmonella challenge experiment represent an important milestone in understanding the genetics of infectious disease resistance, offer a theoretical basis for breeding SP-resistant chicken lines using marker-assisted selection, and provide new information for salmonellosis research in humans and other animals
Abundance and ecological footprint of Pseudoalteromonas phage vB_PhoS_XC in the Ulva prolifera green tide
Pseudoalteromonas is a ubiquitous and abundant genus of marine bacteria commonly associated with algae. In this study, a novel siphoviral-morphological bacteriophage, vB_PhoS_XC, was isolated from the coastal seawaters of Qingdao (China) during a bloom of the Ulva prolifera (U. prolifera) green tide. The morphology of this phage (icosahedron head 51 ± 1 nm in diameter; a tail length of 86 ± 1 nm) was characterized through transmission electron microscope. The biological properties of this virus showed a short latent period (45 minutes), a large burst size (241 virions per cell) and a relatively wide range of temperatures/pH level tolerance (-20°C to 45°C and pH 4 to pH 10, respectively). The vB_PhoS_XC has a 46,490-bp double-stranded DNA genome with a G+C content of 40.0%, and encodes 72 open reading frames (ORFs). Thirty-five of these ORFs were assigned into known functions based on BLAST-based algorithm against NR database of GenBank. In addition, eco-genomic analysis provides the evidence of vB_PhoS_XC accompanied by bloom of U. prolifera, and confirmed the high expression of two phosphatase-metabolism-related auxiliary metabolic genes (AMGs). This study provides new insights into the functional and ecological roles of the Pseudoalteromonas phage vB_PhoS_XC, shedding light on the virological study approach combined with traditional isolation and meta-omics data
Recommended from our members
Bijections for Mahonian statistics on permutations and labeled forests
We undertake a study of bijections which are used to enumerate sets of permutations and labeled forests according to various statistics. A permutation statistic is called Mahonian if it has the same distribution on the symmetric group S\sb{n} as the inversion statistic. The major index and inversion index are the fundamental examples of Mahonian statistics. The inversion index has been extended by Mallows and Riordan to labeled forests. Recently, Bjorner and Wachs generalized the major index to labeled forests and showed that the major index has the same distribution as the inversion index on labeled forests of fixed shape. We give a direct combinatorial proof of this result by constructing an explicit bijection on labeled forests which takes the major index to the inversion index. For the symmetric group this bijection reduces to a new bijection on S\sb{n} taking the major index to the inversion index which is similar to a bijection of Foata. We also generalize the Mahonian statistics of Rawlings and Kadell to labeled forests and show that they have the same distribution as the inversion index as well. Generalizations of the Foata bijection on permutations are also presented
Relationship between the Composition and Elastic Modulus of TiZrTa Alloys for Implant Materials
The elastic modulus is a key factor influencing the applications of implant materials because of the weakening effect of stress shielding. Ti and its alloys are good potential implant materials thanks to their low elastic modulus and fine biocompatibility. The addition of alloying elements into pure Ti and Ti alloys is the basic way to further decrease the elastic modulus whilst simultaneously enhancing strength, wearability, and corrosion resistance, for example. Finding the relationship between the composition and elastic modulus can greatly promote the development of Ti alloys with a low modulus for implant applications. In the current work, we investigated the elastic modulus of TiZrTa alloys with scores of compositions by using the high-throughput diffusion couple method, nanoindentation, and an electron probe micro-analysis. The relationship between the elastic modulus and the composition of the TiZrTa alloys was obtained. The average valence electron theory was employed to make clear the variation between the elastic modulus and the composition. Finally, the composition range formulae of TiZrTa alloys likely to have a low modulus were established by combining our data and previous results. These findings are helpful in developing new Ti alloys with a low modulus and also help to further understand the alloying theory
Reducing the Influence of Systematic Errors in Interference Core of Stepped Micro-Mirror Imaging Fourier Transform Spectrometer: A Novel Calibration Method
The stepped micro-mirror imaging Fourier transform spectrometer (SIFTS) has the advantages of high throughput, compactness, and stability. However, the systematic errors in the interference core of the SIFTS have a significant impact on the interferogram and the reconstructed spectrum. In order to reduce the influence of systematic errors, a transfer error model of the systematic errors in the interference core of the SIFTS is established, and an interferogram and spectrum calibration method is presented, which combines the least squares fitting calibration and the row-by-row fast Fourier transform-inverse fast Fourier transform (FFT-IFFT) flat-field calibration. The experimental results show that the methods can sufficiently reduce the influence of systematic errors in the interference core of the SIFTS, such as the interferogram fringe tilt, the peak position shift of the reconstructed spectrum, and the error of spectral response
Microstructure, mechanical properties and corrosion behavior of additively-manufactured Fe–Mn alloys
In this paper, we describe the effects of different scanning speeds (600–900 mm/s) on the microstructure, mechanical properties and corrosion behavior of biodegradable bone-substitution alloys produced from 80:20 (by wt.) Fe:Mn powders using laser powder bed fusion (LPBF). Both the Mn content (18.9–15.1 wt.% Mn) and density (7920–7730 kg/m3) of the LPBFed samples decreased slightly with increasing laser scanning speed, while the oxygen content increased (0.12–0.40 wt.%). Increasing scanning speed also led to increased porosity (from 0.27% to 2.5%) and increased cracking. The specimen produced at the lowest scanning speed of 600 mm/s, which consisted of only the HCP ε-martensite phase, showed by far the highest yield strength (YS) at 644 MPa and the highest ultimate tensile strength (UTS) at 857 MPa, but the lowest elongation to failure (El) of only 13.7%. Specimens produced at higher scanning rates consisted of both BCC α′-martensite and ε-martensite phases. The sample fabricated at a scanning speed of 700 mm/s showed the best balance of mechanical properties with a YS of 330 MPa, a UTS of 839 MPa, and an El of 36.1%. Electrochemical testing showed corrosion rates from 0.09 mm/yr (600 mm/s specimen) to 0.22 mm/yr (700 mm/s specimen), which are higher than those of both pure Fe and most Fe–30Mn and Fe–35Mn alloys reported in the literature. The work demonstrates that the meso-/micro-scale structure, and, hence, the mechanical properties and corrosion rates of Fe–Mn alloys can be tailored by varying the scanning speed during LPBF processing. It also demonstrates the potential of LPBFed Fe–Mn alloys with low Mn content for use as biodegradable bone substitutes
Surface Microstructure and Performance of Anodized TZ30 Alloy in SBF Solution
Anodization is performed on the Ti-30Zr-5Al-3V (TZ30) alloy to improve its surface performance. X-ray diffractometer (XRD), scanning electron microscopy (SEM), and Olympus microscope are used to determine the phase constitution, morphology, and thickness of the anodization film (AOF). Tribological tests and electrochemical corrosion experiments are carried out to measure, respectively, the wear behavior and corrosion resistance of AOFs in simulated body fluid (SBF) solution. The microstructure characteristic of the AOF anodized at low voltage (20 V) is composed of compact and loose regions. As the applied voltage increases to 60 V, the compact regions transform progressively into loose regions, and then grow into nanotube regions. Besides, an increase in thickness of the AOF from 8.6 ± 4.61 μm to 20.7 ± 2.18 μm, and a gradual increase in surface microhardness from 364.6 ± 14.4 HV to 818.4 ± 19.3 HV, are also exhibited as the applied voltage increases from 20 V to 60 V. Specimens anodized at 40 V and 60 V have a low friction coefficient (~0.15) and wear rate (~2.2 mg/N/m) in the SBF solution. The enhanced wearability originates from the high hardness and various wear mechanisms. Potentiodynamic polarization curves suggest that the corrosion resistance in the SBF solution of all anodized specimens is greatly improved, thanks to the protection from the anodized TiO2 film
RNA-Seq Analysis Reveals Expression Regulatory Divergence of W-Linked Genes between Two Contrasting Chicken Breeds
The regulation of gene expression is a complex process involving organism function and phenotypic diversity, and is caused by cis- and trans- regulation. While prior studies identified the regulatory pattern of the autosome rewiring in hybrids, the role of gene regulation in W sex chromosomes is not clear due to their degradation and sex-limit expression. Here, we developed reciprocal crosses of two chicken breeds, White Leghorn and Cornish Game, which exhibited broad differences in gender-related traits, and assessed the expression of the genes on the W chromosome to disentangle the contribution of cis- and trans-factors to expression divergence. We found that female-specific selection does not have a significant effect on W chromosome gene-expression patterns. For different tissues, there were most parental divergence expression genes in muscle, and also more heterosis compared with two other tissues. Notably, a broader pattern of trans regulation in the W chromosome was observed, which is consistent with autosomes. Taken together, this work describes the regulatory divergence of W-linked genes between two contrasting breeds and indicates sex chromosomes have a unique regulation and expression mechanism