Period2 is associated with immune cell infiltration and is a potential diagnostic and prognostic marker for hepatocellular carcinoma

Background: Hepatocellular carcinoma (HCC) health challenge worldwide. Many studies showed that circadian rhythms play a critical role in tumor development. This study aimed to investigate the role of the circadian gene period2 (PER2) in HCC development and explore the possible mechanisms involved.Methods: From fresh HCC tissues and paired paracancerous tissues, we measured PER2 mRNA and protein expression levels and calculated the correlations between PER2 expression and clinicopathological parameters in patients with HCC. We used transcriptome data from The Cancer Genome Atlas to mine the PER2 gene, including single gene difference analysis, single gene co-expression analysis, gene set enrichment analysis, immune infiltration analysis, and methylation analysis to explore its role and mechanism in HCC occurrence and development.Results: PER2 expression levels were significantly lower in HCC tissues than in the paired paracancerous tissues. PER2 expression in HCC significantly correlated with neural invasion, Child-Pugh classification, and China liver cancer staging stage in HCC patients. The differentially expressed genes associated with PER2 were significantly enriched in mitochondrial oxidative phosphorylation, transcriptional translation, amino acid metabolism, and other related pathways. PER2 expression levels significantly correlated with immune cell infiltration and immune checkpoint genes and positively correlated with TP53 expression in HCC tissues. The DNA methylation status in eight CpG islands of the PER2 gene was associated with HCC outcomes.Conclusion: PER2 is a potential diagnostic and prognostic biomarker and a promising therapeutic target in HCC

CYP2C19 genotype and platelet aggregation test-guided dual antiplatelet therapy after off-pump coronary artery bypass grafting: A retrospective cohort study

BackgroundDual antiplatelet therapy (DAPT) is recommended in patients undergoing off-pump coronary artery bypass graft surgery (OPCAB). Clopidogrel is less effective among patients with loss-of-function (LoF) of CYP2C19 alleles, while ticagrelor has direct effects on P2Y12 receptor. Whether a CYP2C19 genotype plus platelet aggregation test (PAgT)-guided DAPT after CABG could improve clinical outcomes remain uncertain.Materials and methodsFrom August 2019 to December 2020, 1,134 consecutive patients who underwent OPCAB received DAPT for 1 year after surgery in Ruijin Hospital, Shanghai Jiao Tong University School of Medicine. According to the actual treatment they received in real-world, 382 (33.7%) of them received a traditional DAPT: aspirin 100 mg qd + clopidogrel 75 mg qd, no matter the CYP2C19 genotype and response in platelet aggregation test (PAgT). The other 752 (66.3%) patients received an individual DAPT based on CYP2C19 genotype and PAgT: aspirin 100 mg qd + clopidogrel 75 mg qd if CYP2C19 was extensive metabolizer, or moderate metabolizer but normal response in PAgT; aspirin 100 mg qd + ticagrelor 90 mg bid if CYP2C19 was poor metabolizer, or moderate metabolizer but no or low response in PAgT. One-year follow-up was achieved for all patients. The primary outcome was major adverse cardiovascular events (MACE), a composite of cardiovascular death, myocardial infarction, and stroke. The safety outcome was thrombolysis in myocardial infarction (TIMI) criteria major bleeding.ResultsCompared with the traditional DAPT group, the risk of MACE in the individual DAPT group was significantly lower (5.5 vs. 9.2%, HR 0.583; 95% CI, 0.371–0.915; P = 0.019), mainly due to the decreased risk of MI (1.7 vs. 4.2%, HR 0.407; 95% CI, 0.196–0.846; P = 0.016). The risk of TIMI major bleeding events was similar between the two groups (5.3 vs. 6.0%, RR 0.883; 95% CI, 0.537–1.453; P = 0.626).ConclusionFor patients who underwent OPCAB, individual DAPT (CYP2C19 genotype plus PAgT-guided strategy) was associated with a lower risk of MACE and a similar risk of major bleeding

Chronic itch development in sensory neurons requires BRAF signaling pathways

Chronic itch, or pruritus, is associated with a wide range of skin abnormalities. The mechanisms responsible for chronic itch induction and persistence remain unclear. We developed a mouse model in which a constitutively active form of the serine/threonine kinase BRAF was expressed in neurons gated by the sodium channel Nav1.8 (BRAF(Nav1.8) mice). We found that constitutive BRAF pathway activation in BRAF(Nav1.8) mice results in ectopic and enhanced expression of a cohort of itch-sensing genes, including gastrin-releasing peptide (GRP) and MAS-related GPCR member A3 (MRGPRA3), in nociceptors expressing transient receptor potential vanilloid 1 (TRPV1). BRAF(Nav1.8) mice showed de novo neuronal responsiveness to pruritogens, enhanced pruriceptor excitability, and heightened evoked and spontaneous scratching behavior. GRP receptor expression was increased in the spinal cord, indicating augmented coding capacity for itch subsequent to amplified pruriceptive inputs. Enhanced GRP expression and sustained ERK phosphorylation were observed in sensory neurons of mice with allergic contact dermatitis– or dry skin–elicited itch; however, spinal ERK activation was not required for maintaining central sensitization of itch. Inhibition of either BRAF or GRP signaling attenuated itch sensation in chronic itch mouse models. These data uncover RAF/MEK/ERK signaling as a key regulator that confers a subset of nociceptors with pruriceptive properties to initiate and maintain long-lasting itch sensation

Effect of Corrosive Medium and Surface Defect-Energy on Corrosion Behavior of Rolled ZK61M Alloy

Magnesium alloys have been widely used as lightweight engineering structural materials, but their service performances are severely restricted by corrosion failure. In this paper, the influence of corrosive medium and surface defect energy on the corrosion behavior of rolled ZK61M alloy was investigated. The corrosion tests were conducted in different concentrations of sodium chloride solution for different durations, and the polarization curves and electrochemical impedance spectroscopy were reported. The surface morphology of rolled ZK61M alloy before and after corrosion tests were analyzed. The results showed that the corrosion tendency became stronger with the increase of the concentration of corrosive medium and the number of surface defects of ZK61M alloy. Moreover, the initial corrosion pattern was the pitting caused by micro galvanic corrosion at the surface defect, which gradually developed into uniform corrosion. Furthermore, the main damage occurred at the grain boundary, resulting in the destruction of grain bonding force and the removal of material along the rheological layer. The oxidation corrosion mechanism was mainly the anodic dissolution mechanism

Location-based personalized mobile search

Conference Name:9th International Conference on Computer Science and Education, ICCCSE 2014. Conference Address: Vancouver, BC, Canada. Time:August 22, 2014 - August 24, 2014.Location based service (LBS) is widely used with the development of mobile network, and location-based mobile search has become a research hotspot of LBS. However, traditional location-based mobile search technology could only provide the homogeneous results to different users, rather than characteristic results depending on different personal preferences. This paper introduces a collaborative filtering recommendation algorithm - using slop one algorithm for location-based mobile search. Through using an improved slope one algorithm, we can get the results that are closer to users' preferences. As an example, we show a personalized restaurant search application on smart phone

Thermal Management Techniques for Lithium-Ion Batteries Based on Phase Change Materials: A Systematic Review and Prospective Recommendations

Thermal management systems for lithium-ion batteries based on the cooling and heating of phase change materials have become a popular research topic. However, the low thermal conductivity, flame resistance, high and low temperature adaptability of phase change materials, as well as the thermal runaway mechanisms and lightweight design of phase change material-based systems remain to be explored. The aim of this paper is to conduct a publication-wide macro bibliometric review on thermal management systems for lithium-ion batteries based on phase change material to date. Total of 583 associated publications were retrieved from the Web of Science Core Collection database for the period 2006–2022. A bibliometric study was conducted through the visualization software VOSviewer. The findings were derived from annual publication trends, geographical and institutional distribution, authors and their collaborative networks, keyword network analysis and analysis of highly cited publications as well as reference co-citation analysis. The findings provide a comprehensive overview of the evolution of research hotspots in the field and can help researchers who would like to work in the field to quickly grasp the research frontiers and the overall picture. Furthermore, some suggestions for future work are summarized

Design of a Seawater Desalination System with Two-Stage Humidification and Dehumidification Desalination Driven by Wind and Solar Energy

The paper presents a wind–photovoltaic-thermal hybrid-driven two-stage humidification and dehumidification desalination system for remote island regions lacking access to electricity and freshwater resources. By conducting an analysis of the wind and solar energy resources at the experimental site, a suitable wind power station and photovoltaic power station are constructed. The performance of the wind–solar complementary power generation system is then evaluated based on factors such as output power, seawater desalination load power, battery compensation output, system energy consumption, and water production costs. A variable step gradient disturbance method based on the power–duty ratio is proposed for tracking the maximum power point (MPPT) of wind power generation. The output power of the photovoltaic power generation system is optimized, employing a fuzzy logic control (FLC) method to track the MPPT of photovoltaic power generation. This approach effectively addresses the issues of slow speed and low accuracy encountered by traditional MPPT algorithms in tracking the maximum power point (MPP) of both photovoltaic and wind power generations. In order to ensure that the desalination system can operate stably under different weather conditions, eight working modes are designed, and a programmable logic controller (PLC) is used to control the system, which provides a guarantee for stable water production. Experimental results demonstrate that the system exhibits stable performance, achieving a maximum water output of 80.63 Kg/h and daily water yield is 751.32 Kg, the cost of desalination equipment is 1.4892 USD/t

Data_Sheet_1_CYP2C19 genotype and platelet aggregation test-guided dual antiplatelet therapy after off-pump coronary artery bypass grafting: A retrospective cohort study.docx

BackgroundDual antiplatelet therapy (DAPT) is recommended in patients undergoing off-pump coronary artery bypass graft surgery (OPCAB). Clopidogrel is less effective among patients with loss-of-function (LoF) of CYP2C19 alleles, while ticagrelor has direct effects on P2Y12 receptor. Whether a CYP2C19 genotype plus platelet aggregation test (PAgT)-guided DAPT after CABG could improve clinical outcomes remain uncertain.Materials and methodsFrom August 2019 to December 2020, 1,134 consecutive patients who underwent OPCAB received DAPT for 1 year after surgery in Ruijin Hospital, Shanghai Jiao Tong University School of Medicine. According to the actual treatment they received in real-world, 382 (33.7%) of them received a traditional DAPT: aspirin 100 mg qd + clopidogrel 75 mg qd, no matter the CYP2C19 genotype and response in platelet aggregation test (PAgT). The other 752 (66.3%) patients received an individual DAPT based on CYP2C19 genotype and PAgT: aspirin 100 mg qd + clopidogrel 75 mg qd if CYP2C19 was extensive metabolizer, or moderate metabolizer but normal response in PAgT; aspirin 100 mg qd + ticagrelor 90 mg bid if CYP2C19 was poor metabolizer, or moderate metabolizer but no or low response in PAgT. One-year follow-up was achieved for all patients. The primary outcome was major adverse cardiovascular events (MACE), a composite of cardiovascular death, myocardial infarction, and stroke. The safety outcome was thrombolysis in myocardial infarction (TIMI) criteria major bleeding.ResultsCompared with the traditional DAPT group, the risk of MACE in the individual DAPT group was significantly lower (5.5 vs. 9.2%, HR 0.583; 95% CI, 0.371–0.915; P = 0.019), mainly due to the decreased risk of MI (1.7 vs. 4.2%, HR 0.407; 95% CI, 0.196–0.846; P = 0.016). The risk of TIMI major bleeding events was similar between the two groups (5.3 vs. 6.0%, RR 0.883; 95% CI, 0.537–1.453; P = 0.626).ConclusionFor patients who underwent OPCAB, individual DAPT (CYP2C19 genotype plus PAgT-guided strategy) was associated with a lower risk of MACE and a similar risk of major bleeding.</p