Influence of the interaction between nodal fibroblast and breast cancer cells on gene expression

Our aim was to evaluate the interaction between breast cancer cells and nodal fibroblasts, by means of their gene expression profile. Fibroblast primary cultures were established from negative and positive lymph nodes from breast cancer patients and a similar gene expression pattern was identified, following cell culture. Fibroblasts and breast cancer cells (MDA-MB231, MDA-MB435, and MCF7) were cultured alone or co-cultured separated by a porous membrane (which allows passage of soluble factors) for comparison. Each breast cancer lineage exerted a particular effect on fibroblasts viability and transcriptional profile. However, fibroblasts from positive and negative nodes had a parallel transcriptional behavior when co-cultured with a specific breast cancer cell line. The effects of nodal fibroblasts on breast cancer cells were also investigated. MDA MB-231 cells viability and migration were enhanced by the presence of fibroblasts and accordingly, MDA-MB435 and MCF7 cells viability followed a similar pattern. MDA-MB231 gene expression profile, as evaluated by cDNA microarray, was influenced by the fibroblasts presence, and HNMT, COMT, FN3K, and SOD2 were confirmed downregulated in MDA-MB231 co-cultured cells with fibroblasts from both negative and positive nodes, in a new series of RT-PCR assays. In summary, transcriptional changes induced in breast cancer cells by fibroblasts from positive as well as negative nodes are very much alike in a specific lineage. However, fibroblasts effects are distinct in each one of the breast cancer lineages, suggesting that the inter-relationships between stromal and malignant cells are dependent on the intrinsic subtype of the tumor

Avaliação epidemiológica, clínica e molecular de enteropatógenos causadores de diarreia aguda em crianças e adultos residentes na comunidade Quilombola do Abacatal, Ananindeua, Pará

Acute diarrheal disease is a major cause of morbidity and mortality in developing countries and one of the factors that contributes to the worsening of the nutritional status of children. This study aimed to evaluate the clinical, epidemiological and molecular profile of infections by viral and parasitic agents in children aged 0-10 years and those over 10 years of quilombo of Abacatal in the 2008-2010 period. Fecal samples from 294 children were collected in the age group 0-10 years and 81 individuals over 10 years, residents of the community Abacatal, Ananindeua, Pará, which had acute diarrhea board or without diarrhea (controls). The viral diagnosis was made by immunochromatographic and molecular tests and parasitological by Faust and Hoffman method. A total of 375 fecal samples were obtained from 177 individuals. The frequency of viral agents in this study were rotavirus group A rotavirus C and picobirnavirus group by 6.4% (24/375), 0.3% (1/375) and 1.3% (5/375 ), respectively.The polyacrylamide gel electrophoresis confirmed the presence of rotavirus in 23 of 10 samples (43.48%) having short profile of 13 (56.5%) long profile. The presence of intestinal parasites was observed in 272 (77.94%) samples, and the most common were Ascaris lumbricoides detected in 13.18% (46/349) of the samples, followed by Trichuris trichiura with 10.88% (38 / 349), hookworms with 4.01% (14/349) and Strongyloides stercoralis 1.72% (6/349). Of the 24 samples positive for rotavirus group A the following genotypes were detected: G2P [4] (12.50%, 3/24); G1P [8] (25.00%, 6/24), G3P [9] (29.20%, 7/24) and G12P [6] (33.33%, 8/24). Two new genotypes were detected for VP6 genes (I18) and NSP1 (A19) of rotavirus A. Nutritional assessment of 38 children was conducted, showing that 18 4% (7/38) presented malnourished. This study highlights the need to implement preventive actions in the community, including education measures for health, vaccination against rotavirus, and even the implementation of programs to control parasitic infestations.CNPq - Conselho Nacional de Desenvolvimento Científico e TecnológicoA doença diarreica aguda é uma das principais causas de morbidade e mortalidade infantil nos países em desenvolvimento e um dos fatores que mais contribui para o agravamento do estado nutricional das crianças. Este estudo objetivou avaliar o perfil clínico, epidemiológico e molecular das infecções por agentes virais e parasitários em crianças na faixa etária de 0 a 10 anos e da comunidade quilombola do Abacatal, no período de 2008 a 2010. Foram colhidas amostras fecais de 294 crianças na faixa etária de 0 a 10 anos e 81 indivíduos acima de 10 anos, residentes na comunidade do Abacatal, Ananindeua, Pará, que apresentaram quadro de diarreia aguda ou sem diarreia (controles). O diagnóstico viral foi realizado pelos testes imunocromatográficos e moleculares e o parasitológico pelo método de Faust e Hoffman. Um total de 375 amostras de fezes foi obtido de 177 indivíduos. As frequências dos agentes virais encontrados no presente estudo foram rotavírus do grupo A, rotavírus do grupo C e picobirnavírus em 6,4% (24/375), 0,3% (1/375) e 1,3% (5/375), respectivamente. A eletroforese em gel de poliacrilamida confirmou a presença de rotavírus A nas 23 amostras com 10 (43,48%) apresentando perfil curto e 13 (56,52%), perfil longo. A presença de enteroparasitas foi observada em 272 (77,94%) amostras, sendo que os mais frequentes foram Ascaris lumbricoides detectado em 13,18% (46/349) das amostras, seguido por Trichuris trichiura com 10,88% (38/349), ancilostomídeos com 4,01% (14/349) e Strongyloides stercoralis 1,72% (6/349). Das 24 amostras positivas para rotavírus do grupo A foram detectados os seguintes genótipos: G2P[4] (12,50%, 3/24); G1P[8] (25,00%, 6/24), G3P[9] (29,20%, 7/24) e G12P[6] (33,33%, 8/24). Foram detectados dois novos genótipos para os genes VP6 (I18) e NSP1 (A19) de rotavírus A. Foi realizada a avaliação nutricional de 38 crianças, demonstrando que 18,4% (7/38) apresentavam-se desnutridas. Este estudo destaca a necessidade de implementar ações de prevenção na comunidade, incluindo medidas de educação para a saúde, a vacinação contra o rotavírus, e até mesmo a implementação de programas para controlar infestações parasitárias

Detection, epidemiology and characterization of VP6 and VP7 genes of group D rotavirus in broiler chickens

Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, BrasilMinistério da Agricultura. Laboratório Nacional Agropecuário. Belém, PA, Brazil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Rotaviruses infect humans and animals and are classified into eight groups (A to H). Group D rotavirus (RVD) has been described in birds, although relatively few reports are available. The present study focused on RVD, including epidemiological and molecular aspects of samples collected from broiler chickens in the state of Pará, Brazil. A total of 85 faecal samples were collected between 2008 and 2011 from 37 chicken farms located in eight different municipalities. The viral double-stranded RNA was extracted from faecal suspensions and analysed using polyacrylamide gel electrophoresis (PAGE), followed by reverse transcriptase-polymerase chain reaction (RTPCR) and nucleotide sequencing of the VP6 and VP7 genes. Comparing the positive results, 16.5 per cent (14/85) were obtained by PAGE and 35.3 per cent (30/85) by RT-PCR. Samples from seven of eight municipalities were positive for RVD and infections were recorded in 17 (45.9 per cent) of 37 chicken farms. The RVD infection rate was significantly higher in the 16-day to 30-day age group (62.2 per cent; 23/37) compared with other ages. No consistent relationship was found between the infection rate and either the population density in poultry houses or the climatic conditions. The nucleotide sequences of the VP6 gene were 89.9 to 90.9 per cent similar to the prototype strain 05V0049 and were 88.3 to 100 per cent similar among themselves; VP7 gene nucleotide sequences were 84.3 to 85.4 per cent similar to the prototype strain 05V0049 and 93.8 to 100 per cent similar among themselves. Overall, this study provides new insights into the epidemiology and genome characterization of group D rotaviruse

Gastroenterite por rotavírus do grupo A em Leopardus tigrinus e Leopardus pardalis (Carnivora: Felidae) órfãos na Amazônia Oriental

Fundação para a Ciência e a Tecnologia (Portugal), through the research project GHTM– UID/Multi/04413/2013; and Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (Brazil), for the Ph.D. scholarship of Áurea Martins Gabriel.Universidade Nova de Lisboa. Instituto de Higiene e Medicina Tropical. Lisboa, Portugal / Universidade Federal do Pará. Instituto de Ciências Biológicas. Programa de Pós-Graduação em Biologia de Agentes Infecciosos e Parasitários. Belém, PA, BrasilUniversidade Federal Rural da Amazônia. Laboratório de Patologia Animal. Belém, PA, Brasil.Ministério da Saúde. Secretaria de Ciência, Tecnologia, Inovação e Insumos Estratégicos. Instituto Evandro Chagas. Centro Nacional de Primatas. Ananindeua, PA, Brasil / Fundação Oswaldo Cruz. Instituto de Ciência e Tecnologia em Biomodelos. Rio de Janeiro, RJ, Brasil.Ministério da Saúde. Secretaria de Ciência, Tecnologia, Inovação e Insumos Estratégicos. Instituto Evandro Chagas. Laboratórios de Vírus Gastroentéricos. Ananindeua, PA, BrasilUniversidade Federal do Pará. Núcleo de Medicina Tropical. Laboratório de Biologia Molecular e Celular. Belém, PA, Brasil.Ministério da Saúde. Secretaria de Ciência, Tecnologia, Inovação e Insumos Estratégicos. Instituto Evandro Chagas. Laboratórios de Vírus Gastroentéricos. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Ciência, Tecnologia, Inovação e Insumos Estratégicos. Instituto Evandro Chagas. Laboratórios de Vírus Gastroentéricos. Ananindeua, PA, Brasil.Rotaviruses are highly infectious and transmitted by the fecal-oral route, representing a leading cause of diarrheal deaths in children in developing countries. Additionally, it causes significant economic impacts as an enteropathogen among domestic animals. This report presents clinical and diagnostic findings in two cases of rotavirus (RV) infection involving orphaned Leopardus tigrinus (Schreber, 1775) and Leopardus pardalis (Linnaeus, 1758), representing the first registered group A RV (RVA) infection in these species. Both felids were rescued in Pará State, Amazon Region, by the Brazilian Institute of Environment and Renewable Natural Resources and treated in an intensive care ward in a public Environmental Park of Belém City. After adaptation up to the 40th day of the quarantine period, these animals had no typical symptoms of acute, fulminant gastroenteritis. The collected samples were examined at the Virology Laboratory of Instituto Evandro Chagas. RVA antigen was detected in the blood and fecal samples of L. tigrinus by enzyme-linked immunosorbent assay and immunochromatography. RVA-positive samples were subjected to polyacrylamide gel electrophoresis and reverse transcriptase polymerase chain reaction for VP4 and VP7 genes using conventional set primers but with negative results. Stools were examined for ova and parasites yielding negative results and for intestinal endoparasites, yielding negative results. The animals died within a few days following a clinical exacerbation, therefore unresponsive to treatment. Necropsies and histopathological analysis were performed at the Laboratory of Veterinary Pathology of Universidade Federal Rural da Amazônia. Despite the pathologic findings typical of RV infection hemorrhagic enteritis was unusual if presumed etiology is considered

Detection of a novel recombinant strain of norovirus in na African-descendant community from the Amazon region of Brazil in 2008

This study was funded by the Foudation for Research Support of the State of Pará (Fundação de Amparo à Pesquisa do Estado do Pará - Secretaria de Estado de Desenvolvimento, Ciência e Tecnologia) grant code MS/CNPQ/SECTAM – 001/2006, agreement 032/2007, and by Evandro Chagas Institute, Secretary of Health Surveillance (IEC/ SVS), Ministry of Health, BrazilMinistério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Noroviruses, a major cause of acute gastroenteritis outbreaks worldwide, are constantly evolving. Thisability is reflected in the speed and efficiency with which these viruses spread and remain in the human population.The present study reports the detection of a novel recombination event among norovirus genotypes in Brazil in2008. A strain detected in a stool sample from a child with norovirus-associated gastroenteritis, residing in an Africandescendant semi-closed community of Paraº State, wascharacterized as a novel intergenotype recombinant, GII.7/ GII.20, as determined by partial sequencing and SimPlot analysi

Detection of avian group D rotavirus using the polymerase chain reaction for VP6 gene

This study was partially supported by a grant from Pará State Secretary of Science and Technology, contract no. 067/2008 (SEDECT/FAPESPA/PA).Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Agricultura. Laboratório Nacional Agropecuário. Belém, PA, Brazil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Group D rotaviruses (RVs-D) have been documented in birds and, while they may be common in these animals, few molecular studies are available for this specific group. In this study, specific primers for the gene that encodes for the RVs-D VP6 protein were designed and used in a reverse transcription polymerase chain reaction (RT-PCR). Thirty pools of samples were tested by polyacrylamide gel electrophoresis (PAGE) yielding a 30% (9/30) positivity. These pools were subjected subsequently to RT-PCR, with a 53% (16/30) positivity rate. The sensitivity of the PCR assay was demonstrated up to a dilution of 5 × 10−4 ng/L (0.5 pg/L) of the cloned VP6 gene. The four samples were sequenced and showed 90.8–91.1% similarity with regards to the RVs-D VP6 gene. To assess for specificity our RT-PCR was applied to nine samples known to contain enteric viral agents other than group D rotaviruses including picobirnavirus, rotavirus group A, and reovirus with negative results. Overall, the data confirm the specificity of the primers used for detecting the RVs-D by RT-PCR, suggesting that this assay can be used for diagnostic purposes

Varicella-zoster virus: identification of genotypes in cases of varicella and herpes zoster in the Municipalities of Ananindeua, Belém and Marituba, Pará State, Brazil

Universidade Federal do Pará. Núcleo de Medicina Tropical. Belém, PA, Brasil / Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Universidade Federal do Pará. Núcleo de Medicina Tropical. Belém, PA, Brasil / Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Universidade Federal do Pará. Núcleo de Medicina Tropical. Belém, PA, Brasil / Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Este é um estudo do tipo transversal, em que o critério clínico para inclusão das amostras foi a suspeita clínica e/ou aparecimento dos sintomas da varicela ou herpes-zoster para identificação dos genótipos do vírus da varicela-zoster (VVZ), por meio das técnicas de reação em cadeia da polimerase (PCR) e sequenciamento, com posterior pesquisa de polimorfismo de nucleotídeo único (SNP). De novembro de 2008 a março de 2012, foram coletadas 93 amostras (85 casos de varicela e oito de herpes-zoster) de swab nasal, oral e de secreção de vesículas rompidas em vários sítios anatômicos (49 de indivíduos do sexo masculino e 44 do feminino). As estirpes Dumas e Oka parental foram incluídas como referência padrão dos genótipos europeu (classe 1 ou 3) e japonês (classe 2), respectivamente. Estirpes com variabilidade no SNP foram classificadas dentro de um grupo de genótipo denominado mosaico (classe 5). As classes 1 ou 3 foram identificadas em 11 amostras do VVZ, nove casos de varicela e dois casos de herpes-zoster, enquanto que duas amostras apresentaram o genótipo de classe 5, ambos casos de varicela. O genótipo classe 1 ou 3 foi encontrado nos Municípios de Ananindeua, Belém e Marituba, Estado do Pará, Brasil, sendo que o genótipo classe 5 apenas em Ananindeua. A PCR foi sensível em 13 amostras de material direto de vesículas, enquanto que em amostras de saliva não se obteve sucesso.This is a cross-sectional study in which the clinical criteria for inclusion of the samples was the clinical suspicion and/or onset of symptoms of chickenpox or shingles in order to genotype varicella-zoster virus (VZV) by the polymerase chain reaction (PCR) and sequencing techniques, with a search of single nucleotide polymorphisms (SNP) subsequently. From November 2008 to March 2012, 93 samples (85 cases of varicella and eight herpes zoster) were collected from oral, nasal swab and secretion of ruptured vesicles in several anatomical sites (49 male and 44 female). Dumas and Oka parental strains were included as European genotypes of the reference standard (class 1 or 3) and Japanese (class 2) genotypes, respectively. Strains with the SNP variability were classified into a group of genotype describe as Mosaic (class 5). Classes 1 or 3 were identified in 11 samples of VZV, nine cases of varicella and two cases of herpes zoster, while two samples showed the class genotype 5, both cases of varicella. The genotype class 1 or 3 was found in the Municipalities of Ananindeua, Belém and Marituba, Pará State, Brazil, and genotype class 5 only in Ananindeua. PCR was sensitive in just 13 samples of direct vesicles material, but in saliva samples there were not positive results

Detection, epidemiology and characterization of VP6 and VP7 genes of group D rotavirus in broiler chickens

Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, BrasilMinistério da Agricultura. Laboratório Nacional Agropecuário. Belém, PA, Brazil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Rotaviruses infect humans and animals and are classified into eight groups (A to H). Group D rotavirus (RVD) has been described in birds, although relatively few reports are available. The present study focused on RVD, including epidemiological and molecular aspects of samples collected from broiler chickens in the state of Pará, Brazil. A total of 85 faecal samples were collected between 2008 and 2011 from 37 chicken farms located in eight different municipalities. The viral double-stranded RNA was extracted from faecal suspensions and analysed using polyacrylamide gel electrophoresis (PAGE), followed by reverse transcriptase-polymerase chain reaction (RTPCR) and nucleotide sequencing of the VP6 and VP7 genes. Comparing the positive results, 16.5 per cent (14/85) were obtained by PAGE and 35.3 per cent (30/85) by RT-PCR. Samples from seven of eight municipalities were positive for RVD and infections were recorded in 17 (45.9 per cent) of 37 chicken farms. The RVD infection rate was significantly higher in the 16-day to 30-day age group (62.2 per cent; 23/37) compared with other ages. No consistent relationship was found between the infection rate and either the population density in poultry houses or the climatic conditions. The nucleotide sequences of the VP6 gene were 89.9 to 90.9 per cent similar to the prototype strain 05V0049 and were 88.3 to 100 per cent similar among themselves; VP7 gene nucleotide sequences were 84.3 to 85.4 per cent similar to the prototype strain 05V0049 and 93.8 to 100 per cent similar among themselves. Overall, this study provides new insights into the epidemiology and genome characterization of group D rotaviruse

Phylogenetic analysis of probable non-human genes of group A rotaviruses isolated from children with acute gastroenteritis in Belém, Brazil

National Council for Scientific and Technological Development (CNPq), Brasília, Federal District, Brazil. Grant Number: 4742742008‐0Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Department of Virology. Rio de Janeiro, RJ, Brazil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Department of Virology. Rio de Janeiro, RJ, Brazil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Rotaviruses (RVs) are the main cause of acute viral gastroenteritis in both humans and young animals of various species such as calves, horses, pigs, dogs, cats, and birds. The genetic diversity of RVs is related to a variety of evolutionary mechanisms, including point mutation, and genome reassortment. The objective of this study was to characterize molecularly genes that encode structural and nonstructural proteins in unusual RV strains. The clinical specimens selected for this study were obtained from children and newborn with RV gastroenteritis, who participated in research projects on viral gastroenteritis conducted at the Evandro Chagas Institute. Structural (VP1-VP4, VP6, and VP7) and nonstructural (NSP1-NSP6) genes were amplified from stool samples by the polymerase chain reaction and subsequently sequenced. Eight unusual RV strains isolated from children and newborn with gastroenteritis were studied. Reassortment between genes of animal origin were observed in 5/8 (62.5%) strains analyzed. These results demonstrate that, although rare, interspecies (animal-human) transmission of RVs occurs in nature, as observed in the present study in strains NB150, HSP034, HSP180, HST327, and RV10109. This study is the first to be conducted in the Amazon region and supports previous data showing a close relationship between genes of human and animal origin, representing a challenge to the large-scale introduction of RV vaccines in national immunization programs