51 research outputs found
MIBiG 3.0 : a community-driven effort to annotate experimentally validated biosynthetic gene clusters
With an ever-increasing amount of (meta)genomic data being deposited in sequence databases, (meta)genome mining for natural product biosynthetic pathways occupies a critical role in the discovery of novel pharmaceutical drugs, crop protection agents and biomaterials. The genes that encode these pathways are often organised into biosynthetic gene clusters (BGCs). In 2015, we defined the Minimum Information about a Biosynthetic Gene cluster (MIBiG): a standardised data format that describes the minimally required information to uniquely characterise a BGC. We simultaneously constructed an accompanying online database of BGCs, which has since been widely used by the community as a reference dataset for BGCs and was expanded to 2021 entries in 2019 (MIBiG 2.0). Here, we describe MIBiG 3.0, a database update comprising large-scale validation and re-annotation of existing entries and 661 new entries. Particular attention was paid to the annotation of compound structures and biological activities, as well as protein domain selectivities. Together, these new features keep the database up-to-date, and will provide new opportunities for the scientific community to use its freely available data, e.g. for the training of new machine learning models to predict sequence-structure-function relationships for diverse natural products. MIBiG 3.0 is accessible online at https://mibig.secondarymetabolites.org/
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AC Losses in the MICE Channel Magnets -- Is This a Curse or a Blessing?
This report discusses the AC losses in the MICE channel magnets during magnet charging and discharging. This report talks about the three types of AC losses in the MICE magnets; the hysteretic AC loss in the superconductor, the coupling AC loss in the superconductor and the eddy current AC loss in the magnet mandrel and support structure. AC losses increase the heat load at 4 K. The added heat load increases the temperature of the second stage of the cooler. In addition, AC loss contributes to the temperature rise between the second stage cold head and the high field point of the magnet, which is usually close to the magnet hot spot. These are the curses of AC loss in the MICE magnet that can limit the rate at which the magnet can be charge or discharged. If one is willing to allow some of the helium that is around the magnet to boil away during a magnet charge or discharge, AC losses can become a blessing. The boil off helium from the AC losses can be used to cool the upper end of the HTS leads and the surrounding shield. The AC losses are presented for all three types of MICE magnets. The AC loss temperature drops within the coupling magnet are presented as an example of how both the curse and blessing of the AC losses can be combined
Triacylglycerol utilization is required for regrowth of in vitro hypoxic nonreplicating Mycobacterium bovis bacillus Calmette-Guerin
10.1128/JB.00530-09Journal of Bacteriology191165037-5043JOBA
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