Effects of dietary protein and energy levels on growth performance, feed utilization and body composition of juvenile shirbot Barbus grypus (Heckle, 1843)

Shirbot (Barbus grypus) as a species with high potential for aquaculture development recently propagated artificially in South Iran Aquaculture Research Center to extend the species diversity in polyculture system. To provide an effective economic diet for this species 9 experimental diets containing three crude protein levels (250, 300, and 350 g kg -1) and three metabolizable energy levels (10.46, 12.55 and 14.64 MJ kg -1) were fed to triplicate groups of shirbot juvenile fish (initial body weight of 29.68± 0.19). Three aerated tanks were randomly assigned to each treatment, stocked with 15 juvenile fish and reared for a 60-day period. The preferential diet, which was diet 4, (300 g kg -1 CP and 10.46 MJ kg -1 ME) exhibited the best growth and feed utilization performances. Fishes fed diet 4 showed higher weight gain, feed efficiency ratio and survival rate with a significant difference (P0.05). It was found that varying levels of CP and DE in the diets did not significantly affect the body composition of the fish (P>0.05) except for the CF. Comparison between varying levels of dietary protein and energy on the growth, feed utilization and body composition of Barbus grypus indicated that 250 to 300 g kg -1 CP and 10.46 MJ kg -1 ME could be the preferential dietary levels for this species in the juvenile stage

Effects of weight and length of Barbus grypus broodstock on fingerlings production and growth

The effects of weight and length of Barbus grypus broodstock on fingerlings reproductive characteristics and growth were investigated. We chose three female treatments including weight and length groups (1) 2212.5±479g, 62.68±6.7cm, (2) 4518±780g, 79.12±4.36cm and (3) 7712.5±1171g, 92.62±3.13cm. The same male broodstock were used for all the treatments with the weight and length size of 1400±100g, 54.62±7.35cm. Female broodstock were injected 3mg/kg PG hormone two times at an interval of 10 hours. Males were injected 2mg/kg once at the time females received their second injection. Larvae and fingerling survival rate and growth indices (special growth coefficient, condition factor) up to the fingerling stage were calculated for all treatments of larvae culture. Fingerling stage in ponds, 02, pH, temperature, transparency, nitrate, and phosphate were measured and no significant difference was observed (P>0.05) among the ponds. The special growth rate, condition factor, and fingerling survival of the treatments showed no significant differences (P>0.05). However, our results indicated that larvae survival rate had significant differences between treatment 2 and other treatments (P<0.05). These indices increase in broodstock weight up to treatment 2 (4518±780g, 79.12±4.36cm) and after that it will be lowered. We conclude that the treatment 2 broodstock are ideal for fingerling survival and enhancement of growth and condition factors

Correlation between length-weight and age in Barbus sharpeyi and Barbus grypus broodstocks in artificial propagation

This study was carried out to identify proper broodstock of Barbus sharpeyi and Barbus grypus using working fecundity indices. For Barbus sharpeyi three female groups were chosen based on previous experiences that included treatment 1: 650±300g, 1±0.5 years, (T.L) 40.68±2.7cm, treatment 2: 1350±350g, 2.5±0.5 years, (T.L) 47.92±4.63cm and treatment 3: 2200±400g, 4±0.4 years, and (T.L) 59.62±3.56cm. The same male broodstock (700±80g) and 2 year old fish were used for all treatments. For Barbus grypus three female groups were chosen using previous experiences that included treatment 1: 2212.5±780g, 2.5±0.5 years, (T.L) 62.68±6.7cm, treatment 2: 4518±780g, 4±1 years (T.L) 79.12±4.36cm and treatment 3: 7712.5±171g, 7.7±1.3 years and (T.L) 92.62±3.13cm. The same male broodstock (1400±100g) and 2 year old fish were used for all treatments. Female broodstock of the two species were injected 3mg/kg of PG hormone two times at an interval of 10 hours and males were injected 2mg/kg. The maximum working fecundity was seen in treatment 2 for Barbus sharpeyi (33000±1450) and Barbus grypus and (13000.37±4651.57). Functional fecundity were calculated for Barbus sharpeyi in all treatments which was significantly different between treatment 2 and other treatments for the two species. After this stage, the working fecundity was decreased in treatments. We conclude that treatment 2 is the best for selection of broodstocks in the two fish species

Determination of dietary protein and energy level s and optimum lipid to carbohydrate ratio for Gattan (barbus xanthopterus) fingerlings

This study was done is three phase ,to required suitable level of protein and energy and optimum carbohydrate to lipid ratio (B.xanthopterus) fingerling . Phase one: Determine of dietary protein and energy l eve in tanks (300 liters) treatment with triplicate e in different level of protein (25 ,30 ,35 percentage) and three level of energy (250 ,300 ,350 kcal/gr) the result showed that the best (GSR and WG) belongs to diet with protein % 35 and 300 kcal/gr. this experiment showed with increased of protein , SGR and WG increased and the one special level of protein with increased of energy level ,first SGR and WG increased (the most of SGR belonged 300 kcal/gr) ,and SGR decreased. Phase 2 : Determine optimum lipid to carbohydrate ratio in tanks (300 liters) In this phase with suitable level of protein and energy( protein %35 and 300kcal/gr) determined 5 ratio of carbohydrate and lipid (0.8, 2.8, 4.86, 8.8 ) in this phase best diet determined with 4/8 ratio , and 6/8 , 2/8 ratios were after that carbohydrate and lipid ratio. Phase 3: Determine optimum lipid to carbohydrate ratio in pond: In this phase, the result of thirty phase were like second phase. we determined that the best SGR and WG and WG % belonged to 4/8 carbohydrate and lipid ratio and 6/8 2/8 ratio were after 4/8 carbohydrate and lipid rate

Study on specific growth and food behaviour of Barbus grypus in system of monoculture and polyculture

During this project Barbus grypus was cultured in monoculture and polyculture methods With Cyprinus carpio, Ctenopharyndon idellus, Hypophthlmichth molitrix and Arystichthys Nobilis fishes. The result indicates that Barbus grypus in polycuture method has special growth rate, higher weight and length, some amount of different nutrition item were used. In integrated culture system due to physicochemical condition growth was suitable

Brood production of Barbus xanthopterus by fingerling rearing

This study has been carried out to determine the possibility of producing Gattan fish (Barbus xanthopterus) brooders in captivity condition. For this reason during in year 2004-2005, 600 specimen of Gattan in three pond and in 2008, from two local point shilat and research center, 200 specimen in two ponds were placed. The area of each pond was 1800 square meters. In the first period of rearing, total weight and total length of nearly two years old brooders reached to 989 g and 44 cm respectively. All of the fishes were male (except one case) and they had become mature. In second rearing period, 27 percent of shilat specimens and 42 percent of research center specimens were female. In 22 months old fishes, the males and females of shilat specimens reached to mean weight 716 and 658 g and mean length 382 and 365 cm respectively. Also, the male and females of research center specimens reached to mean weight was 1195 and 11064 g and mean length 443 and 425 cm respectively, when they were 22 months old. The absolute and specific growth rate in two rearing periods showed ascending trend and the 100 percent of brooders were survived. The male fishes were matured in 2 years old and the mature female fishes were not observed. The brooders response to pituitary extract in research center specimens was found to be more than shilat specimens

Using pellt in grass carp Ctenopharygodon idella

For hand feeding of Grass carp (Ctenopharyngodon idella), a kind of fish used in integrated carp culture, two kinds of pellets with Alfa alfa as current food were suggested as follows: 1. Pellet B with low fiber and high protein 2. Pellet C with high fiber and low protein The ingredient ratio was powdered and mixed, by adding water, fiber was made and minced the minced pellet was put on net tray and then in blotter to be dried and ready for use. In this experiment food quality analysis and also food water stability was measured. Regarding sampling, growth rate and weight increase of fish and FCR of each ratio was determined. Results indicates diet C is not able to compete with Alfa alfa from the stand point of weight increase, FCR and price. But diet B with low FCR and higher growth rate and higher price (not meaning ful P 5%) can compete with Alfa alfa

Determination of optimum levels of energy and protein on pacific white shrimp (Litopenaeus vannamei) diet at marketing stage

To investigate an economical diet for white pacific prawn (Litopenaeus vannamei) rearing coastal zones of south Iran, 9 experimental diets were prepared considering three protein (37%, 37%, 40% CP) and three digestible energy (262, 312, 362 kcal. 100g^-1 DE) levels, using herbal protein source (soybean meal) as main protein source for the diets. 27 polyethylene round tanks (300 L volume) provided with aerated flow-through water system, stocked with …juvenile shrimps (9.16 ± 0.05 g mean weight) were applied to evaluate the growth performance of the shrimps and feed efficiency parameters of the diets for 8 weeks rearing period in an in-door experimental system. Results showed the diets were significantly different (P≤0.05)in the terms of growth performance and diet efficiency the shrimp, while the best obtaining observed in diet 1 (34% CP and 262 kcal. 100g^-1 DE). Average of weight gain was ranged from 4.58 ± 0.52 g in Diet 9 up to 6.88 ± 0.81 g in diet one, SGR was from 0.73 ± 0.06 in diet 9 to 1.00 ± 0.09 in diet one, survival was from 75.00 ± 7.07 % in diet 9 to 95.00 ± 5.00 % in diet one and FCR was from 5.02 ± 0.94 in diet one to 8.24 ± 1.59 in diet 9. Diets did not effectively influenced the body composition of the shrimp (P>0.05). According to the results of the present study, 34% Cp and 262 kcal. 100g^-1 DE could be suggested as suitable levels to be considered in the diet of the white pacific prawn in the grow-out stage

Survey of different dietary energy and protein levels on the reproduction indices of Benni (Barbus sharpeyi) broodstock

The survey of different protein and energy levels of brooders Benni (Mesopotamichthys sharpeyi) diet on reproductive indices This study, to preparation of suitable diet for brooders of Benni (Mesopotamichthys sharpeyi) carried Out increasing efficiency artificial propagation and achievement to maximum working fecundity and fingerling production. 9 dietary feeding with 3 triplication were prepared that included 3 protein levels (30,35 and 40 percentage) and 3 different levels of digestable energy (250, 300 and 350 kcal/100g).The reproductive indices (working fecundity, fertilization, hatching and survival rate) were studied in this experiment in South Iran Aquaculture Research Center .12 female brooders in any triplication were transported to earthen ponds 300 m2. They were fed 2 times in days for 4 month until satiation. The artificial breeding carried out in spring for survey of reproductive indices. The amount of hypophysis injection was 3mg/kg weight of fish with two doses10% in first stage and 90% in second stage with interval 10 Hour. The male broodstock injection was first stage spantanously with second females injections with a dose of 2 mg/kg. The total of breeding stages until larvae release was registered 22.5-24.5°C. Result showed that constant protein (35%) with rising energy until specified amount (350 Kcal/100g) was increased reproductive indicies. The working fecundity is one of the reproductive indices that in treatment 5 had significant difference compare to other treatment. In this survey, Comparison between different levels of dietary protein and energy on the brooders Benni indicated that 35% protein level and 350 Kcal/100g energy for barbus grypus had best efficiency in diet of broodstock

Effect of protein and energy levels of diet on the growth parameters of shirbot (Barbus grypus) in the grow out stage

This study was done is two phases, to determine required suitable level of protein and energy for Barbus grypus to get maximum growth to market size. Phase 1-finger ling phase Treatments with triplicate in different level of protein (25, 30, 35 percentage) and different level of digestible energy (kcal/100 gr 250,300,350) were used. Also the effect of mentioned diets on fish growth by calculating (WG, FER, FCR, SVR, PER, SGR) was studied. 9 treatments with triplicate random group of 15 fish per 300 liter tank the mean of were stuclied for 60 days. Body weight was (29.68±0.19gr). The results showed that in constant protein levels growth parameters improved to a special level with in Creasing energy level but in constant. Protein decrease with in crase in energy level. The result of caracass showed energy, protein, fiber and lipid caracass increased with increase in protein and energy, ash level of diet, on the other hand the mentioned diets had meaningfull effect on biochemical composition of caracass except (protein and ash). The best protein level was (%30) and suitable energy level was (250 Kcal/100gr). Phase 2: Grow out stage The best diets (P30%) and (250Kcal/100gr) and (p350%),(300kcal/100gr),(p35%)(350Kcal/100gr)was determind of second phase. These three suitable diets 3 treament in a soil pond 160m² with primary weight 98.3±23 in 60 day. Siqnificant different bet ween treatments were obsereved based on WG,FER,FCR,SVR,PER,SGR Indices. There were no Siqnificant different in (fish body composition)in second phases of experiment. The level of energy,protein,fiber and lipid. In fishes body were increased with in increasing the diet protein. Finally, according to mean valves,30% protein level and (250 Kcal/100gr) energy for barbus grypus are suggestedias the best levels