Isolation decomposing bacteria of polycyclic aromatic hydrocarbons (PAHs) from Tashk Lake and examination of salt concentration effect on them

Tashk Lake is located in the protected zone of the Bakhtegan Lake in the Fars Province and is a shelter for wildlife. This place is very important environmentally and ecologically. Among entering pollutants to this lake via rivers and side villages, polycyclic aromatic hydrocarbons (PAHs) compounds are noteworthy which are very harmful to humans and others creatures alike. We used naphthalene and anthracene as the only source of carbon to isolate PAHs degrading bacteria from three predefined stations. We found Pseudomonas sp. as the only PAHs degrading bacterium in the water and sediments of the lake. At the station 1 (Dehzir), the mean isolated bacteria acting on naphthalene and anthrecene were 27 and 23 in each ml of water and 57 and 47 in each gram of sediments, respectively. At the station 2 (Tashk), the mean isolated bacteria were 67 and 57 in each ml of water and 120 and 113 in each gram of the sediments which acted on the two carbon sources respectively. At the station 3 (Gomban), the figures were 127 and 113 for water and 163 and 147 for the sediments respectively

Isolation and identification of phenol degrading bacteria from Lake Parishan and their growth kinetic assay

Phenol and its components are extremely toxic and can easily be isolated from different industrial sewage such as oil refinery, petrochemical industry and mines, especially collier and chemical factories. Hence the presence of these compounds in the environment could cause environmental pollution, especially in water resources. In the past, physicochemical method was used for the elimination of phenol and its compounds, but today, bioremediation is preferable. The aim of this study is to isolate and identify phenol degrading bacteria from Lake Parishan and to assay their kinetic growth. Sixty samples of water and sedimentation of different area of Lake Parishan were collected. In order to isolate phenol degrading bacteria, samples were cultured on salt base phenol broth media. For screening of degrading bacteria, bromothymole blue indicator was added to media, which formed green color in it. Finally, the ability of bacteria to degrade different concentration of phenol was measured using culturing bacteria in different concentration of phenol from 0.2 to 0.9 g/l. Cultivated bacteria on the salt base phenol broth containing indicator changed the color of the media from green to yellow by using the phenol and decreasing the pH. These bacteria were, chiefly, gram negative and they belong to Pseudomonaceae and Acinetobacteraceae Family. Psuodomonas spp. are the most important phenol degrading bacteria in Lake Parishan which showed vast diversity in different parts of this lake. Species of Acinetobacter and other species such as Kelibsiella, Citrobacter and Shigella were found as well. Most of the isolated bacteria showed a good ability of degradation of phenol, where Pseudomonas and Acinetobacter showed 0.8 - 0.9 g/l, and Kelibsiella, Citrobacter and Shigella showed 0.6 - 0.7 g/l and the rest showed 0.2 - 0.3 g/l of phenol degradation. Findings show that the Lake Parishan has a lot of high ability phenol degrading bacteria. The most important species belong to Psuodomonas and Acinetobacter.Key words: Phenol, degrading, Pseudomonas, Acinetobacter, Lake Parishan

The effect of processed cereals or molasses on digestion in the rumen and nutrient supply to the duodenum of heifers fed grass silage

SIGLEAvailable from British Library Document Supply Centre- DSC:DX172666 / BLDSC - British Library Document Supply CentreGBUnited Kingdo

ISOLATION DECOMPOSING BACTERIA OF POLYCYCLIC AROMATIC HYDROCARBONS (PAHS) FROM TASHK LAKE AND EXAMINATION OF SALT CONCENTRATION EFFECT ON THEM

Tashk Lake is located in the protected zone of the Bakhtegan Lake in the Fars Province and is a shelter for wildlife. This place is very important environmentally and ecologically. Among entering pollutants to this lake via rivers and side villages, polycyclic aromatic hydrocarbons (PAHs) compounds are noteworthy which are very harmful to humans and others creatures alike. We used naphthalene and anthracene as the only source of carbon to isolate PAHs degrading bacteria from three predefined stations. We found Pseudomonas sp. as the only PAHs degrading bacterium in the water and sediments of the lake. At the station 1 (Dehzir), the mean isolated bacteria acting on naphthalene and anthrecene were 27 and 23 in each ml of water and 57 and 47 in each gram of sediments, respectively. At the station 2 (Tashk), the mean isolated bacteria were 67 and 57 in each ml of water and 120 and 113 in each gram of the sediments which acted on the two carbon sources respectively. At the station 3(Gomban), the figures were 127 and 113 for water and 163 and 147 for the sediments respectively. We found meaningful differences in the mean number of degrading bacteria in the water and sediments of the three stations (P<0.05). The salt concentration for Pseudomonas sp. optimum growth and PAHs degradation was found to be around 6% with and increasing rate of degradation over time

Variety effect on composition, kinetics of fermentation and in vitro digestibility of oat (Avena sativa L.) straw and its neutral detergent fibre

Yield, chemical composition, in vitro digestibility and kinetics of fermentation of straw from 18 varieties of oats (Avena sativa L.) were studied. All the straw varieties were grown in three replicates under the same agronomic conditions. Significance differences were observed in the yield of straw (4.4 to 7.5 ton dry matter (DM)/ha) from different varieties. The proportion of seed/straw from these varieties varied from 0.28 to 1.02. Crude protein (CP), neutral detergent fibre (NDF), acid detergent fibre (ADF) and acid detergent lignin (ADL) content varied from 24.2 to 48.1, 626 to 708, 437 to 533 and 52.0 to 92.4 g/kg DM, respectively in the straws. In vitro organic matter digestibility (IVOMD) differed among varieties and varied from 400 to 539 g/kg DM. The mean value of digestible organic matter yield (DOM) was 2.34 ton/ha. A significant difference was observed in the potential gas production (A) and lag time (L) among varieties. The fractional rate of gas production (c, /h) ranged from 0.030 to 0.034. The results emphasized that in any evaluation of oat varieties, kinetics of digestion or fermentation should be taken into consideration as well as yield and digestibility.Keywords: Oats, straw yield, composition, digestibility, gas production kinetic

A newly isolated Streptomyces rimosus strain capable of degrading deltamethrin as a pesticide in agricultural soil

Chemical pesticides or insecticides with complex structures are highly abundant in the biosphere and have inevitable side effects on farmland, natural resources, and human health. Deltamethrin is the most popular and widely used pesticide that disrupts the cellular calcium channels. In the present study, isolated strains of bacteria were examined to determine the ones that were capable of degrading deltamethrin. Different species of bacteria were evaluated in terms of the capability to degrade deltamethrin. It is important to note that Streptomyces rimosus was able to degrade up to 200 mg/L deltamethrin concentration and could be grown in mineral salt medium agar containing deltamethrin to be used as a source of carbon and energy. The results demonstrated that there is a diversity of deltamethrin-degrading bacteria in agricultural soil ecosystems. The application of these bacteria, especially S. rimosus, might be used as a bioremediation technique to decrease pesticide contamination of the ecosystem. © 2020 WILEY-VCH Verlag GmbH & Co. KGaA, Weinhei

Comparison of two molecular diagnostic methods for identifying beijing genotype of mycobacterium tuberculosis

Background and Objectives: The Beijing family of Mycobacterium tuberculosis has been identified as a severe pathogen among this species and found in many clinical isolates during the last decade. Early identification of such genotype is important for better prevention and treatment of tuberculosis. The present study performed to compare the efficiency of Real-Time PCR and IS6110-Based Inverse PCR methods to identify the Beijing family. Materials and Methods: This study was carried out on 173 clinical isolates of Mycobacterium tuberculosis complex in Golestan Province, northern Iran. DNA extraction performed by boiling and determining the Beijing and non-Beijing strains carried out using Real-Time PCR and IS6110-Based Inverse PCR. Results: In both Real-Time PCR and IS6110-Based Inverse PCR method, 24 specimens (13.9) of the Beijing family were identified and the result of the IS6110-Based Inverse PCR method showed that all the Beijing strains in this region belonged to the Ancient Beijing sub-lineage. Conclusion: Although the efficacy of the two methods in the diagnosis of the Beijing family is similar, the IS6110-Based Inverse PCR is more applicable to the ability to detect new and old Beijing family. © 2020, Tehran University of Medical Science. All rights reserved

Crocin promotes apoptosis in human EBV-transformed b-lymphocyte via intrinsic pathway

Background: As a major carotenoid in saffron, crocin demonstrates potent anti-cancer impacts. However, its anti-lymphoma effects remain vague, especially in the human EBV-associated B-cell lymphoproliferative disorders. This study examined crocin's apoptogenic potential and its underlying mechanism in CO 88BV59-1 cell line vs. normal human peripheral blood B cells. Methods: CO 88BV59-1 cells were treated with crocin alone or in combination with vincristine for up to 72 h. The cell viability was examined using a resazurin assay. Flow cytometry using annexin V and propidium iodide labeling was performed to detect apoptotic cells. Also, the expression levels of genes and proteins involved in apoptosis (CASP3, CASP8, CASP9, P53, Bax, and Bcl-2) were respectively determined via real-time PCR and Western blot analysis. Results: Crocin concentration-dependently reduced cell viability in CO 88BV59-1 cells with no significant toxicity toward normal B cells. Similar to vincristine, crocin significantly increased apoptosis in these cells during 72 h of incubation. Furthermore, the combination of crocin (80 μM) and vincristine (1 μM) enhanced apoptosis in CO 88BV59-1 cells. Therefore, this synergistic effect was detected in human EBV-transformed B-lymphocyte. CASP3, CASP9, P53, and Bax/Bcl-2 ratio expressions were significantly raised in CO 88BV59-1 cells, whereas CASP8 was unaltered. It was proposed that crocin promoted apoptosis in CO 88BV59-1 cells in a time- and concentration-dependent manner via the induction of the intrinsic pathway. Conclusion: The results suggest that crocin may serve as a good alternative/coadjuvant to vincristine in EBV-associated B-cell lymphoproliferative disorders. © 2021 Universita Cattolica del Sacro Cuore. All rights reserved