Bacterial community analysis of sediment seep in Kagoshima Bay, Japan

1902-1906Microorganisms in the deep-sea environments such as hydrothermal vent and cold-seep regions are primary energy producers and an important community in these ecosystems. We have used 454-Pyrosequencing and 16S rDNA clone library methods to determine the diversity of bacteria in the sediment of the seep regions around the vestimentiferan tubeworm habitat at Kagoshima Bay. Taxonomic composition from both libraries suggested that 454-Pyrosequencing methods can represent more diverse groups than the conventional clone library methods. Most abundant taxa with higher folds were Proteobacteria and Bacteroidetes found in both methods. Through the 454-Pyrosequencing method, we were able to detect underrepresented taxa as well as non-detectable taxa. This analyses and comparison provide bacterial taxonomic group detection efficiency of both library types and emphasize the different uses and utilities for exploring the unknown microbial domain

The complete mitochondrial genome of the sand dollar Astriclypeus mannii (Verrill, 1867) (Echinoidea: Astriclypeidae) in the subtidal sand flat in Jeju Island off the south coast of Korea

We sequenced the complete mitochondrial genome of sand dollar Astriclypeus mannii (Verrill 1867) (Echinoidea: Astriclypeidae) occurring in the subtidal sand flat in Jeju Island off the south coast of Korea. The mitochondrial genome was 15,744 bp in length and contained 13 protein-coding genes (PCGs), 22 transfer RNAs, two ribosomal RNAs, and 140 nucleotides representing the putative control region. We reconstructed the concatenated phylogenetic tree based on 13 PCGs of 18 echinoderms, including A. mannii. From the maximum likelihood clustering, A. mannii was grouped in the order Echinolampadacea. The complete mitochondrial sequence of A. mannii for the first time in this study provide valuable insight in understanding the evolution and phylogenetic analysis of echinoids (sea urchins)

Complete Genome Sequence of “Candidatus Puniceispirillum marinum” IMCC1322, a Representative of the SAR116 Clade in the Alphaproteobacteria▿

The complete genome sequence of “Candidatus Puniceispirillum marinum” IMCC1322, the first cultured representative of the SAR116 clade in the Alphaproteobacteria, is reported here. The genome contains genes for proteorhodopsin, aerobic-type carbon monoxide dehydrogenase, dimethylsulfoniopropionate demethylase, and C1 compound metabolism. The genome information proposes the SAR116 group to be metabolic generalists in ocean nutrient cycling