Impact of coccidial infection on vaccine- and vvIBDV in lymphoid tissues of SPF chickens as detected by RT-PCR

BACKGROUND: This study aimed at investigating a potential effect caused by coccidia on the immune response to vaccine- and very virulent infectious bursal disase virus (vvIBDV) in SPF chickens. METHODS: Two groups of three weeks old SPF chickens were vaccinated prior to inoculation with coccidia and challenge with virulent IBDV, all within a period of eight days. Two control groups were similarly treated, except that challenge with field virus was omitted in one group while inoculation with coccidia was omitted in the other group. Clinical signs, lesions in the intestines caused by coccidia, lesions in the bursa of Fabricius caused by IBDV, IBDV-antibody titres, and virus detection by reverse transcription polymerase chain reaction (RT-PCR) were compared among the groups. Lymphoid tissues and swab samples were analysed by general RT-PCR, and positive results were identified by strain specific duplex (DPX) RT-PCR. RESULTS: In the tripple-infected groups, vaccine strain IBDV was detected in spleen and thymus tissues, and no field virus was detected in bursa samples, contrary to the double-infected groups. CONCLUSION: The results suggest an enhancing effect on the immune response caused by subclinical coccidiosis and vvIBDV acting in concert

Detection of vvIBDV in Vaccinated SPF Chickens

The purpose of our experiment was to investigate, if apparently healthy, vaccinated chickens may be involved in maintaining and spreading infectious bursal disease virus (IBDV) in poultry environments. We aimed at simultaneous detection and identification of very virulent field strain IBDV (vvIBDV) as well as vaccine strain IBDV in experimentally infected chickens. Two groups of specific pathogen free (SPF) chickens were vaccinated using the intermediate infectious bursal disease (IBD) vaccine D78. Group 1 was vaccinated at the age of one week and group 2 at the age of three weeks. Both groups were challenged with vvIBDV at the age of four weeks. A third, vaccinated, non-challenged group served as negative control. No clinical symptoms were observed in any of these groups. The chickens were euthanised and submitted to autopsy and sample preparation in groups of three at fixed intervals from the age of 28 to 44 days. Gross pathological lesions were not observed. Lymphoid tissues from the bursa of Fabricius, bone marrow, spleen and thymus in addition to cloacal- and bursal swaps were analysed by one-step reverse transcription polymerase chain reaction (RT-PCR). Positive results were confirmed by two-step strain specific duplex (DPX) RT-PCR. The vaccine strain was detected in bursa tissues from all groups, while the challenge strain was detected in few bursal as well as non-bursal tissue samples. The results indicate a possibility of replication of vvIBDV in vaccinated chickens

A simple algorithm to detect balance in signed graphs

We develop a natural correspondence between marked graphs and balanced signed graphs, and exploit it to obtain a simple linear time algorithm by which any signed graph may be tested for balance.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/23150/1/0000075.pd

First introduction of highly pathogenic H5N1 avian influenza A viruses in wild and domestic birds in Denmark, Northern Europe

This is an Open Access article distributed under the terms of the Creative Commons Attribution Licens

Development and External Validation of the International Early Warning Score for Improved Age- and Sex-Adjusted In-Hospital Mortality Prediction in the Emergency Department

Objectives: Early Warning Scores (EWSs) have a great potential to assist clinical decision-making in the emergency department (ED). However, many EWS contain methodological weaknesses in development and validation and have poor predictive performance in older patients. The aim of this study was to develop and externally validate an International Early Warning Score (IEWS) based on a recalibrated National Early warning Score (NEWS) model including age and sex and evaluate its performance independently at arrival to the ED in three age categories (18-65, 66-80, &gt; 80 yr). Design: International multicenter cohort study. Setting: Data was used from three Dutch EDs. External validation was performed in two EDs in Denmark. Patients: All consecutive ED patients greater than or equal to 18 years in the Netherlands Emergency department Evaluation Database (NEED) with at least two registered vital signs were included, resulting in 95,553 patients. For external validation, 14,809 patients were included from a Danish Multicenter Cohort (DMC). Measurements and Main Results: Model performance to predict in-hospital mortality was evaluated by discrimination, calibration curves and summary statistics, reclassification, and clinical usefulness by decision curve analysis. In-hospital mortality rate was 2.4% (n = 2,314) in the NEED and 2.5% (n = 365) in the DMC. Overall, the IEWS performed significantly better than NEWS with an area under the receiving operating characteristic of 0.89 (95% CIs, 0.89-0.90) versus 0.82 (0.82-0.83) in the NEED and 0.87 (0.85-0.88) versus 0.82 (0.80-0.84) at external validation. Calibration for NEWS predictions underestimated risk in older patients and overestimated risk in the youngest, while calibration improved for IEWS with a substantial reclassification of patients from low to high risk and a standardized net benefit of 5-15% in the relevant risk range for all age categories. Conclusions: The IEWS substantially improves in-hospital mortality prediction for all ED patients greater than or equal to18 years.</p

Mapping the UK research & innovation landscape: Energy & development

The UK is already a major player in terms of research and innovation into low carbon transitions within the countries of the developing world. However, there are significant opportunities for the UK to enhance its presence within these markets whilst also making a major contribution to meeting international development targets and climate commitments. At the core of the research analysed in the report is that it reflects disparate, research initiatives funded with different research/innovation targets in mind; much latent strength in UK research for low carbon energy for development therefore is implicit in linkage possibilities enhancing systemic effectiveness, particularly by cross-fertilizing innovations taking place in the private sector. Gaps and weaknesses are reflected as much in the lack of collaborative initiatives and ‘siloization’ as in the absence of actual research/funding. The Low Carbon Energy for Development Network (LCEDN) is currently taking the initiative in one aspect of this through the provision of a programme of capacity building and partnership activities to support the development of DfID’s Transforming Energy Access research initiative. What is required of UK energy for development research for the purposes of building a functional system, however, is that the discrete areas of research outlined in this report be re-assembled as coherent, overall research narratives addressing the apparent contradiction of increasing energy access whilst transitioning to the low-carbon economy. Part of this work involves identifying the state and dynamics of UK research capacity in this sector, facilitating greater integration between research funders active in this area, evaluating gaps and key research needs and mapping potential future directions for research interventions and collaborations that build on, and develop, existing UK research capacity. It is expected that this will lead to a range of UK-led energy innovations developed, tested and scaled across developing countries by 2020. The much-needed harmonization of energy access and low carbon transition as a UK research theme however has ultimately to be created out of rethinking research fields from a combination of existing research, plus demand known to be ‘out there’ but which has yet to enter the field of vision of research-funders. This report presents the first part of this work with an overview of current UK research and innovation capacity in a widely defined ‘Energy and International Development’ research area. It identifies key institutions and research centres, thematic areas of excellence, research funding trends over the last decade, emerging research themes plus an overview of grant funding for innovation on the ground. A number of key areas/questions for potential further development of UK research and innovation capacity have been identified and are up for discussion and consultation. The work has been undertaken by LCEDN in partnership with the Knowledge Transfer Network, Energy 4 Impact and IOD PARC