37 research outputs found
STRUCTURAL TRANSFORMATIONS IN AXILLARY AND MESENTERIC LYMPH NODES IN CHEMOTHERAPY AND SURGICAL TREATMENT OF EXPERIMENTAL MAMMARY TUMOR
Was conducted histological study axillary and mesenteric lymph nodes in breast cancer induced by intramammaryΒ administration of N-methyl-N-nitrosourea, chemotherapy according to the CMF scheme (cyclophosphamide,Β methotrexate, 5-fluorouracil), operative removal of breast tumors (6.5 months from the beginning of the experiment).Β The results of the study. At chemotherapy of breast cancer, compared with the group with breast cancer withoutΒ treatment, there was a decrease in the number of tumor cells in the axillary lymph nodes in comparison with mesentericΒ lymph nodes. The decrease in the area of the paracortical zone and the area of secondary lymphoid nodes remain in theΒ axillary lymph nodes, in comparison with breast cancer without treatment. The reduction of the paracortical zone squareΒ remains in mesenteric lymph nodes. The area of lymphoid nodules with germinative centers decreases. The numberΒ of postcapillary venules with high endothelium and the number of macrophages in structural zones grow down. In theΒ axillary lymph nodes after surgical treatment of breast cancer and chemotherapy in comparison with the treatment ofΒ breast cancer only with cytostatics, there is decrease in the area of the paracortical zone (with an increase in the numberΒ of small lymphocytes) and medullare cords. The area of lymphoid nodules with germinative and without germinativeΒ centers increases. In mesenteric lymph nodes, drainage function is reduced, increased the area of the paracortical zone,Β reduced the areas of lymphoid nodules with germinative centers and medullare cords (increased proliferative activity ofΒ cells), macrophage reaction in the cortical substance was revealed. Conclusion. The severity of structural transformationsΒ in cytoarchitectonics of the axillary and mesenteric lymph nodes depends on the treatment method
CORRELATION BETWEEN CYTOKINE CONTENT IN LYMPH OF THORACIC LYMPH DUCT AND MESENTERIC LYMPH NODE STRUCTURAL TRANSFORMATIONS IN EXPERIMENTAL MAMMARY TUMOR AND CHEMOTHERAPY
The aim of the study was to fulfill correlation analysis of morphometry of the mesenteric lymph nodes and the concentrationΒ of cytokines in the lymph of the thoracic duct in breast cancer induced by intramammary administration of N-methyl-N-nitrosourea, chemotherapy according to the CMF scheme (cyclophosphamide, methotrexate, 5-fluorouracil). TheΒ results of the study. At breast cancer revealed positive correlation: in the germinative centers and medullary cords ofΒ cytokine IL-5 with mitotically dividing cells, chemokines MIP-1Ξ± with average lymphocytes, in the germinative centersΒ of immunoblasts with cytokine GRO/KC, in the paracortical zone chemokine MCP-1 with macrophages, reticular cellsΒ with IL-6 and M-CSF, in the medullary sinuses chemokine GRO/KC with small lymphocytes and mature plasma cellsΒ (number which decreases). All this may indicate the activity of the local immune response in the lymph nodes aimed onΒ the antitumor protection. After chemotherapy of breast cancer, compared with breast cancer without treatment, revealedΒ positive relationship, which may indicate increased immunomodulatory and antitumor actions of cytokines: correlationΒ of interferon IFNΞ³ with small lymphocytes (number which increased) and macrophages in the germinative centers andΒ mitotically dividing cells in the medullary cords, correlation in the germinative centers of immunoblasts with MIP-1Ξ± and increased of number small lymphocytes in T-dependent zone lymph nodes, correlation in medullary cords ofΒ interleukin IL-17 with mature plasma cells (number which increased) , correlation of interleukin IL-18 with matureΒ plasma cells in medullary sinuses. Conclusion. Study of the correlation of the concentration of cytokines in the lymphΒ of the thoracic duct with structural changes in the mesenteric lymph nodes revealed dependencies aimed at increasingΒ the immunomodulating and antitumor effects of cytokines
Π£Π ΠΠΠΠ ΠΠΠ ΠΠΠΠΠ, ΠΌΠΈΠΊΡΠΎΠ ΠΠ Π Π¦ΠΠ’ΠΠΠΠΠΠ Π ΠΠΠΠ€Π Π ΠΠΠ ΠΠ Π ΠΠ Π Π ΠΠΠ ΠΠΠΠΠ§ΠΠΠ ΠΠΠΠΠΠ« Π ΠΠΠ‘ΠΠΠ ΠΠΠΠΠ’Π
The involvement of hormones, microRNAs and cytokines in breast cancer pathogenesis has been wellΒ established. Lymph picks up secretory products of breast cancer cells. The purpose of the study was toΒ evaluate the levels of hormones, microRNAs and cytokines in lymph. Wistar rats were injected with N-methyl-N-nitrosourea to induce breast cancer. The rats were subjected to either surgery alone or chemotherapy aloneΒ (cyclophosphamide, methotrexate and 5-fluorouracil). In some animals, surgery was followed by chemotherapy.Β The levels of follicle-stimulating hormone (FSH), prolactin, luteinizing hormone (LH), estradiol (E2) andΒ thyroglobulin (TG), microRNA-21, microRNA-221, microRNA-222, microRNA-429 and 24 cytokines wereΒ determined. Chemotherapy was shown to result in the reduction in the levels of prolactin, thyroglobulin, FSHΒ and estradiol. In rats with breast cancer, the expression levels of microRNA-21, microRNA-221 and microRNA-222 were increased, and the expression levels of microRNA-429 were decreased. In breast cancer rats, theΒ levels of most cytokines were found to be increased. Correlations between the levels of cytokines, hormones,Β and microRNAs in lymph were identified. Differences in the expression levels of cytokines, hormones, andΒ microRNAs in lymph with respect to treatment option were detected.Π¦Π΅Π»Ρ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΡ β ΠΎΡΠ΅Π½ΠΊΠ° ΡΡΠΎΠ²Π½Π΅ΠΉ Π³ΠΎΡΠΌΠΎΠ½ΠΎΠ², ΠΌΠΈΠΊΡΠΎΠ ΠΠ ΠΈ ΡΠΈΡΠΎΠΊΠΈΠ½ΠΎΠ² Π² Π»ΠΈΠΌΡΠ΅.ΠΠ°ΡΠ΅ΡΠΈΠ°Π» ΠΈ ΠΌΠ΅ΡΠΎΠ΄Ρ.Β ΠΠΊΡΠΏΠ΅ΡΠΈΠΌΠ΅Π½ΡΠ°Π»ΡΠ½ΡΠΉ ΡΠ°ΠΊ ΠΌΠΎΠ»ΠΎΡΠ½ΠΎΠΉ ΠΆΠ΅Π»Π΅Π·Ρ ΠΈΠ½Π΄ΡΡΠΈΡΠΎΠ²Π°Π»ΠΈ Π²Π²Π΅Π΄Π΅Π½ΠΈΠ΅ΠΌ N-ΠΌΠ΅ΡΠΈΠ»-N-Π½ΠΈΡΡΠΎΠ·ΠΎΠΌΠΎΡΠ΅Π²ΠΈΠ½Ρ ΡΒ ΠΊΡΡΡ Wistar. Π§Π°ΡΡΡ ΠΆΠΈΠ²ΠΎΡΠ½ΡΡ
ΠΏΠΎΠ΄Π²Π΅ΡΠ³Π°Π»Π°ΡΡ ΡΠΎΠ»ΡΠΊΠΎ ΠΎΠΏΠ΅ΡΠ°ΡΠΈΠ²Π½ΠΎΠΌΡ Π²ΠΌΠ΅ΡΠ°ΡΠ΅Π»ΡΡΡΠ²Ρ ΠΈΠ»ΠΈ ΡΠΎΠ»ΡΠΊΠΎ Ρ
ΠΈΠΌΠΈΠΎΡΠ΅ΡΠ°ΠΏΠΈΠΈ (ΡΠΈΠΊΠ»ΠΎΡΠΎΡΡΠ°Π½, ΠΌΠ΅ΡΠΎΡΡΠ΅ΠΊΡΠ°Ρ, 5-ΡΡΠΎΡΡΡΠ°ΡΠΈΠ»). Π£ ΡΠ°ΡΡΠΈ ΠΆΠΈΠ²ΠΎΡΠ½ΡΡ
ΡΠΎΡΠ΅ΡΠ°Π»ΠΈ ΠΎΠΏΠ΅ΡΠ°ΡΠΈΠ²Π½ΠΎΠ΅ Π²ΠΌΠ΅ΡΠ°ΡΠ΅Π»ΡΡΡΠ²ΠΎ Ρ ΠΏΠΎΡΠ»Π΅Π΄ΡΡΡΠΈΠΌ ΠΊΡΡΡΠΎΠΌ Π₯Π’. Π Π»ΠΈΠΌΡΠ΅ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π»ΠΈ ΡΠΎΠ΄Π΅ΡΠΆΠ°Π½ΠΈΠ΅ ΡΠΎΠ»Π»ΠΈΠΊΡΠ»ΠΎΡΡΠΈΠΌΡΠ»ΠΈΡΡΡΡΠ΅Π³ΠΎΒ Π³ΠΎΡΠΌΠΎΠ½Π° (Π€Π‘Π), ΠΏΡΠΎΠ»Π°ΠΊΡΠΈΠ½Π°, Π»ΡΡΠ΅ΠΈΠ½ΠΈΠ·ΠΈΡΡΡΡΠ΅Π³ΠΎ Π³ΠΎΡΠΌΠΎΠ½Π° (ΠΠ), ΡΡΡΡΠ°Π΄ΠΈΠΎΠ»Π° (Π2) ΠΈ ΡΠΈΡΠΈΠΎΠ³Π»ΠΎΠ±ΡΠ»ΠΈΠ½Π° (Π’Π),Β ΠΌΠΈΠΊΡΠΎΠ ΠΠ-21, ΠΌΠΈΠΊΡΠΎΠ ΠΠ-221, ΠΌΠΈΠΊΡΠΎΠ ΠΠ-222, ΠΌΠΈΠΊΡΠΎΠ ΠΠ-429 ΠΈ 24 ΡΠΈΡΠΎΠΊΠΈΠ½ΠΎΠ².Π Π΅Π·ΡΠ»ΡΡΠ°ΡΡ. ΠΠΎΠΊΠ°Π·Π°Π½ΠΎ,Β ΡΡΠΎ Π½Π° ΡΠΎΠ½Π΅ Π₯Π’ ΡΠ½ΠΈΠΆΠ°ΡΡΡΡ ΡΡΠΎΠ²Π½ΠΈ ΠΏΡΠΎΠ»Π°ΠΊΡΠΈΠ½Π°, ΡΠΈΡΠ΅ΠΎΠ³Π»ΠΎΠ±ΡΠ»ΠΈΠ½Π°, Π€Π‘Π ΠΈ ΡΡΡΡΠ°Π΄ΠΈΠΎΠ»Π°. Π Π³ΡΡΠΏΠΏΠ΅ ΠΆΠΈΠ²ΠΎΡΠ½ΡΡ
Β Ρ Π ΠΠ ΡΠ²Π΅Π»ΠΈΡΠ΅Π½Ρ ΡΡΠΎΠ²Π½ΠΈ ΡΠΊΡΠΏΡΠ΅ΡΡΠΈΠΈ ΠΌΠΈΠΊΡΠΎΠ ΠΠ-21, ΠΌΠΈΠΊΡΠΎΠ ΠΠ-221, ΠΌΠΈΠΊΡΠΎΠ ΠΠ-222 ΠΈ ΡΠ½ΠΈΠΆΠ΅Π½Ρ ΡΡΠΎΠ²Π½ΠΈΒ ΡΠΊΡΠΏΡΠ΅ΡΡΠΈΠΈ ΠΌΠΈΠΊΡΠΎΠ ΠΠ-429. ΠΡΠΈ Π ΠΠ Π² Π»ΠΈΠΌΡΠ΅ ΡΠ²Π΅Π»ΠΈΡΠ΅Π½Ρ ΡΡΠΎΠ²Π½ΠΈ Π±ΠΎΠ»ΡΡΠΈΠ½ΡΡΠ²Π° ΡΠΈΡΠΎΠΊΠΈΠ½ΠΎΠ². ΠΠ΅ΠΆΠ΄ΡΒ ΡΡΠΎΠ²Π½ΡΠΌΠΈ Π² Π»ΠΈΠΌΡΠ΅ ΡΠΈΡΠΎΠΊΠΈΠ½ΠΎΠ², Π³ΠΎΡΠΌΠΎΠ½ΠΎΠ² ΠΈ ΠΌΠΈΠΊΡΠΎΠ ΠΠ ΠΎΠΏΡΠ΅Π΄Π΅Π»Π΅Π½Ρ Π²Π·Π°ΠΈΠΌΠΎΡΠ²ΡΠ·ΠΈ. Π Π»ΠΈΠΌΡΠ΅ Π²ΡΡΠ²Π»ΡΡΡΡΡΒ ΡΠ°Π·Π»ΠΈΡΠ½ΡΠ΅ ΡΡΠΎΠ²Π½ΠΈ ΡΠΈΡΠΎΠΊΠΈΠ½ΠΎΠ², Π³ΠΎΡΠΌΠΎΠ½ΠΎΠ² ΠΈ ΠΌΠΈΠΊΡΠΎΠ ΠΠ Ρ ΡΡΠ΅ΡΠΎΠΌ Π²ΠΈΠ΄Π° ΠΏΡΠΎΠ²Π΅Π΄Π΅Π½Π½ΠΎΠ³ΠΎ Π»Π΅ΡΠ΅Π½ΠΈΡ
ΠΠΏΡΡ ΠΎΠ»Ρ-Π°ΡΡΠΎΡΠΈΠΈΡΠΎΠ²Π°Π½Π½ΡΠ΅ ΠΌΠ΅Π·Π΅Π½Ρ ΠΈΠΌΠ½ΡΠ΅ ΡΡΠ²ΠΎΠ»ΠΎΠ²ΡΠ΅ ΠΊΠ»Π΅ΡΠΊΠΈ ΠΏΡΠΈ Ρ ΠΈΠΌΠΈΡΠ΅ΡΠΊΠΈ-ΠΈΠ½Π΄ΡΡΠΈΡΠΎΠ²Π°Π½Π½ΠΎΠΌ ΡΠ°ΠΊΠ΅ ΠΌΠΎΠ»ΠΎΡΠ½ΠΎΠΈΜ ΠΆΠ΅Π»Π΅Π·Ρ Ρ ΠΊΡΡΡ Wistar
Objective: to compare the morphological and functional properties of mesenchymal stem cells from mammary tissues and chemically-induced mammary tumor tissues. material and methods. The study included 25 female Wistar rats. In 20 rats, mammary carcinoma was induced by intramammary injection of N-methyl-N-nitrosourea after estrus synchronization with chorionic gonadotropin. The control group consisted of 5 rats. Mammary carcinoma was verified histologically and immunohistochemically. To examine, whether the cells isolated from normal tissue and tumor tissue belonged to mesenchymal stem cells, FACS Canto II flow cytofluorometer was used. The functional properties of mesenchymal stem cells were evaluated in MTT assay by the level of nitric oxide production in normal and by hydrogen peroxide-induced hypoxia. The levels of prolactin, luteinizing hormone and estradiol E2 in urine were studied using solid-phase enzyme-linked immunosorbent assay. results. Chemically-induced mammary tumor according to histological and immunohistochemical studies corresponded to luminal B type breast cancer in humans. In rats that developed mammary tumors, the urine prolactin levels after synchronization of estrus were increased. In rats that did not develop tumors, the levels of prolactin and luteinizing hormone were decreased, but the levels of estradiol E2 were increased. More mesenchymal stem cells with CD45-/CD90+phenotype were obtained from the breast tumor tissue. Mesenchymal stem cells from tumor tissue showed increased proliferative potential and were more resistant to hypoxia. conclusion. Tumor- associated mesenchymal stem cells having high proliferative potential and resistance to hypoxia were obtained from chemically-induced mammary tumor tissue. Morphologic and functional differences in mesenchymal stem cells obtained from mammary breast tissue and tumor tissue require further studies.Π¦Π΅Π»Ρ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΡ β ΡΡΠ°Π²Π½ΠΈΡΡ ΠΌΠΎΡΡΠΎΡΡΠ½ΠΊΡΠΈΠΎΠ½Π°Π»ΡΠ½ΡΠ΅ ΡΠ²ΠΎΠΈΜΡΡΠ²Π° ΠΌΠ΅Π·Π΅Π½Ρ
ΠΈΠΌΠ½ΡΡ
ΡΡΠ²ΠΎΠ»ΠΎΠ²ΡΡ
ΠΊΠ»Π΅ΡΠΎΠΊ ΠΈΠ· ΡΠΊΠ°Π½Π΅ΠΈΜ ΠΌΠΎΠ»ΠΎΡΠ½ΠΎΠΈΜ ΠΆΠ΅Π»Π΅Π·Ρ ΠΈ ΡΠΊΠ°Π½ΠΈ Ρ
ΠΈΠΌΠΈΡΠ΅ΡΠΊΠΈ-ΠΈΠ½Π΄ΡΡΠΈΡΠΎΠ²Π°Π½Π½ΠΎΠΈΜ ΠΎΠΏΡΡ
ΠΎΠ»ΠΈ ΠΌΠΎΠ»ΠΎΡΠ½ΠΎΠΈΜ ΠΆΠ΅Π»Π΅Π·Ρ. ΠΌΠ°ΡΠ΅ΡΠΈΠ°Π» ΠΈ ΠΌΠ΅ΡΠΎΠ΄Ρ. Π£ 20 ΠΊΡΡΡ-ΡΠ°ΠΌΠΎΠΊ Wistar ΠΏΠΎΡΠ»Π΅ ΡΠΈΠ½Ρ
ΡΠΎΠ½ΠΈΠ·Π°ΡΠΈΠΈ ΡΡΡΡΡΡΠ° Ρ
ΠΎΡΠΈΠΎΠ½ΠΈΡΠ΅ΡΠΊΠΈΠΌ Π³ΠΎΠ½Π°Π΄ΠΎΡΡΠΎΠΏΠΈΠ½ΠΎΠΌ, ΠΈΠ½ΡΡΠ°ΠΌΠ°ΠΌΠΌΠ°ΡΠ½ΡΠΌ Π²Π²Π΅Π΄Π΅Π½ΠΈΠ΅ΠΌ N-ΠΌΠ΅ΡΠΈΠ»-N-Π½ΠΈΡΡΠΎΠ·ΠΎΠΌΠΎΡΠ΅Π²ΠΈΠ½Ρ ΠΈΠ½Π΄ΡΡΠΈΡΠΎΠ²Π°Π½Π° Π°Π΄Π΅Π½ΠΎΠΊΠ°ΡΡΠΈΠ½ΠΎΠΌΠ° ΠΌΠΎΠ»ΠΎΡΠ½ΠΎΠΈΜ ΠΆΠ΅Π»Π΅Π·Ρ, ΠΊΠΎΠ½ΡΡΠΎΠ»ΡΠ½ΡΡ Π³ΡΡΠΏΠΏΡ ΡΠΎΡΡΠ°Π²ΠΈΠ»ΠΈ 5 ΠΊΡΡΡ. ΠΠ΅ΡΠΈΡΠΈΠΊΠ°ΡΠΈΡ ΠΎΠΏΡΡ
ΠΎΠ»ΠΈ ΠΏΡΠΎΠ²ΠΎΠ΄ΠΈΠ»ΠΈ Π³ΠΈΡΡΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΈ ΠΈ ΠΈΠΌΠΌΡΠ½ΠΎΠ³ΠΈΡΡΠΎΡ
ΠΈΠΌΠΈΡΠ΅ΡΠΊΠΈ. ΠΡΠΈΠ½Π°Π΄Π»Π΅ΠΆΠ½ΠΎΡΡΡ Π²ΡΠ΄Π΅Π»Π΅Π½Π½ΡΡ
ΠΊΠ»Π΅ΡΠΎΠΊ ΠΈΠ· ΡΠΊΠ°Π½ΠΈ ΠΌΠΎΠ»ΠΎΡΠ½ΠΎΠΈΜ ΠΆΠ΅Π»Π΅Π·Ρ ΠΈ ΠΎΠΏΡΡ
ΠΎΠ»ΠΈ ΠΌΠΎΠ»ΠΎΡΠ½ΠΎΠΈΜ ΠΆΠ΅Π»Π΅Π·Ρ ΠΊ ΠΌΠ΅Π·Π΅Π½Ρ
ΠΈΠΌΠ½ΡΠΌ ΡΡΠ²ΠΎΠ»ΠΎΠ²ΡΠΌ ΠΊΠ»Π΅ΡΠΊΠ°ΠΌ Π²Π΅ΡΠΈΡΠΈΡΠΈΡΠΎΠ²Π°Π»ΠΈ Π½Π° ΠΎΡΠ½ΠΎΠ²Π°Π½ΠΈΠΈ ΠΌΠΎΡΡΠΎΠ»ΠΎΠ³ΠΈΠΈ ΠΈ ΡΠ΅Π½ΠΎΡΠΈΠΏΠΈΡΠΎΠ²Π°Π½ΠΈΡ Π½Π° ΠΏΡΠΎΡΠΎΡΠ½ΠΎΠΌ ΡΠΈΡΠΎΡΠ»ΡΠΎΡΠΈΠΌΠ΅ΡΡΠ΅ FACS Canto II. Π€ΡΠ½ΠΊΡΠΈΠΎΠ½Π°Π»ΡΠ½ΡΠ΅ ΡΠ²ΠΎΠΈΜΡΡΠ²Π° ΠΌΠ΅Π·Π΅Π½Ρ
ΠΈΠΌΠ½ΡΡ
ΡΡΠ²ΠΎΠ»ΠΎΠ²ΡΡ
ΠΊΠ»Π΅ΡΠΎΠΊ ΠΎΡΠ΅Π½ΠΈΠ²Π°Π»ΠΈ Π² ΠΠ’Π’-ΡΠ΅ΡΡΠ΅ ΠΈ ΠΏΠΎ ΡΡΠΎΠ²Π½Ρ ΠΏΡΠΎΠ΄ΡΠΊΡΠΈΠΈ ΠΎΠΊΡΠΈΠ΄Π° Π°Π·ΠΎΡΠ° Π² Π½ΠΎΡΠΌΠ΅ ΠΈ ΠΏΡΠΈ ΠΈΠ½Π΄ΡΠΊΡΠΈΠΈ ΠΏΠ΅ΡΠ΅ΠΊΠΈΡΡΡ Π²ΠΎΠ΄ΠΎΡΠΎΠ΄Π° ΠΎΠΊΠΈΡΠ»ΠΈΡΠ΅Π»ΡΠ½ΠΎΠ³ΠΎ ΡΡΡΠ΅ΡΡΠ°. Π£ΡΠΎΠ²Π½ΠΈ ΠΏΡΠΎΠ»Π°ΠΊΡΠΈΠ½Π°, Π»ΡΡΠ΅ΠΈΠ½ΠΈΠ·ΠΈΡΡΡΡΠ΅Π³ΠΎ Π³ΠΎΡΠΌΠΎΠ½Π° ΠΈ ΡΡΡΡΠ°Π΄ΠΈΠΎΠ»Π° Π2 Π² ΠΌΠΎΡΠ΅ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π»ΠΈ ΡΠ²Π΅ΡΠ΄ΠΎΡΠ°Π·Π½ΡΠΌ ΠΈΠΌΠΌΡΠ½ΠΎΡΠ΅ΡΠΌΠ΅Π½ΡΠ½ΡΠΌ Π°Π½Π°Π»ΠΈΠ·ΠΎΠΌ. Π Π΅Π·ΡΠ»ΡΡΠ°ΡΡ. Π₯ΠΈΠΌΠΈΡΠ΅ΡΠΊΠΈ ΠΈΠ½Π΄ΡΡΠΈΡΠΎΠ²Π°Π½Π½Π°Ρ ΠΎΠΏΡΡ
ΠΎΠ»Ρ ΠΌΠΎΠ»ΠΎΡΠ½ΠΎΠΈΜ ΠΆΠ΅Π»Π΅Π·Ρ ΠΏΠΎ Π΄Π°Π½Π½ΡΠΌ Π³ΠΈΡΡΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΎΠ³ΠΎ ΠΈ ΠΈΠΌΠΌΡΠ½ΠΎΠ³ΠΈΡΡΠΎΡ
ΠΈΠΌΠΈΡΠ΅ΡΠΊΠΎΠ³ΠΎ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΡ ΡΠΎΠΎΡΠ²Π΅ΡΡΡΠ²ΠΎΠ²Π°Π»Π° Π»ΡΠΌΠΈΠ½Π°Π»ΡΠ½ΠΎΠΌΡ Π1 ΡΠΈΠΏΡ ΡΠ°ΠΊΠ° ΠΌΠΎΠ»ΠΎΡΠ½ΠΎΠΈΜ ΠΆΠ΅Π»Π΅Π·Ρ Ρ ΡΠ΅Π»ΠΎΠ²Π΅ΠΊΠ°. Π£ΡΠΎΠ²Π½ΠΈ ΠΏΡΠΎΠ»Π°ΠΊΡΠΈΠ½Π° Π² ΠΌΠΎΡΠ΅ ΠΏΠΎΡΠ»Π΅ ΡΠΈΠ½Ρ
ΡΠΎΠ½ΠΈΠ·Π°ΡΠΈΠΈ ΡΡΡΡΡΡΠ° Ρ ΠΆΠΈΠ²ΠΎΡΠ½ΡΡ
Ρ ΡΠ°Π·Π²ΠΈΠ²ΡΠ΅ΠΈΜΡΡ ΠΎΠΏΡΡ
ΠΎΠ»ΡΡ Π±ΡΠ»ΠΈ ΠΏΠΎΠ²ΡΡΠ΅Π½Ρ, Π° Ρ ΠΆΠΈΠ²ΠΎΡΠ½ΡΡ
Ρ Π½Π΅ΡΠ°Π·Π²ΠΈΠ²ΡΠ΅ΠΈΜΡΡ ΠΎΠΏΡΡ
ΠΎΠ»ΡΡ ΠΌΠΎΠ»ΠΎΡΠ½ΠΎΠΈΜ ΠΆΠ΅Π»Π΅Π·Ρ ΡΠ½ΠΈΠΆΠ΅Π½Ρ ΡΡΠΎΠ²Π½ΠΈ ΠΏΡΠΎΠ»Π°ΠΊΡΠΈΠ½Π° ΠΈ Π»ΡΡΠ΅ΠΈΠ½ΠΈΠ·ΠΈΡΡΡΡΠ΅Π³ΠΎ Π³ΠΎΡΠΌΠΎΠ½Π°, Π½ΠΎ ΠΏΠΎΠ²ΡΡΠ΅Π½Ρ ΡΡΠΎΠ²Π½ΠΈ ΡΡΡΡΠ°Π΄ΠΈΠΎΠ»Π° Π2. ΠΠ· ΡΠΊΠ°Π½ΠΈ ΠΎΠΏΡΡ
ΠΎΠ»ΠΈ ΠΌΠΎΠ»ΠΎΡΠ½ΠΎΠΈΜ ΠΆΠ΅Π»Π΅Π·Ρ ΠΏΠΎΠ»ΡΡΠ΅Π½ΠΎ Π±ΠΎΠ»ΡΡΠ΅Π΅ ΠΊΠΎΠ»ΠΈΡΠ΅ΡΡΠ²ΠΎ ΠΌΠ΅Π·Π΅Π½Ρ
ΠΈΠΌΠ½ΡΡ
ΡΡΠ²ΠΎΠ»ΠΎΠ²ΡΡ
ΠΊΠ»Π΅ΡΠΎΠΊ Ρ ΡΠ΅Π½ΠΎΡΠΈΠΏΠΎΠΌ CD45-/CD90+. ΠΠ΅Π·Π΅Π½Ρ
ΠΈΠΌΠ½ΡΠ΅ ΡΡΠ²ΠΎΠ»ΠΎΠ²ΡΠ΅ ΠΊΠ»Π΅ΡΠΊΠΈ ΠΈΠ· ΡΠΊΠ°Π½ΠΈ ΠΎΠΏΡΡ
ΠΎΠ»ΠΈ ΠΏΡΠΎΡΠ²Π»ΡΠ»ΠΈ ΠΏΠΎΠ²ΡΡΠ΅Π½Π½ΡΠΈΜ ΠΏΡΠΎΠ»ΠΈΡΠ΅ΡΠ°ΡΠΈΠ²Π½ΡΠΈΜ ΠΏΠΎΡΠ΅Π½ΡΠΈΠ°Π» ΠΈ Π±ΡΠ»ΠΈ Π±ΠΎΠ»Π΅Π΅ ΡΡΡΠΎΠΈΜΡΠΈΠ²Ρ ΠΊ ΠΎΠΊΠΈΡΠ»ΠΈΡΠ΅Π»ΡΠ½ΠΎΠΌΡ ΡΡΡΠ΅ΡΡΡ. Π·Π°ΠΊΠ»ΡΡΠ΅Π½ΠΈΠ΅. ΠΠ· ΡΠΊΠ°Π½ΠΈ Ρ
ΠΈΠΌΠΈΡΠ΅ΡΠΊΠΈ ΠΈΠ½Π΄ΡΡΠΈΡΠΎΠ²Π°Π½Π½ΠΎΠΈΜ ΠΎΠΏΡΡ
ΠΎΠ»ΠΈ ΠΌΠΎΠ»ΠΎΡΠ½ΠΎΠΈΜ ΠΆΠ΅Π»Π΅Π·Ρ ΠΏΠΎΠ»ΡΡΠ΅Π½Ρ ΠΎΠΏΡΡ
ΠΎΠ»Ρ-Π°ΡΡΠΎΡΠΈΠΈΡΠΎΠ²Π°Π½Π½ΡΠ΅ ΠΌΠ΅Π·Π΅Π½Ρ
ΠΈΠΌΠ½ΡΠ΅ ΡΡΠ²ΠΎΠ»ΠΎΠ²ΡΠ΅ ΠΊΠ»Π΅ΡΠΊΠΈ, ΠΊΠΎΡΠΎΡΡΠ΅ ΠΈΠΌΠ΅ΡΡ Π²ΡΡΠΎΠΊΠΈΠΈΜ ΠΏΡΠΎΠ»ΠΈΡΠ΅ΡΠ°ΡΠΈΠ²Π½ΡΠΈΜ ΠΏΠΎΡΠ΅Π½ΡΠΈΠ°Π» ΠΈ ΡΡΡΠΎΠΈΜΡΠΈΠ²Ρ ΠΊ ΠΎΠΊΠΈΡΠ»ΠΈΡΠ΅Π»ΡΠ½ΠΎΠΌΡ ΡΡΡΠ΅ΡΡΡ. ΠΡΡΠ²Π»Π΅Π½Π½ΡΠ΅ ΠΌΠΎΡΡΠΎΡΡΠ½ΠΊΡΠΈΠΎΠ½Π°Π»ΡΠ½ΡΠ΅ ΡΠ°Π·Π»ΠΈΡΠΈΡ ΠΌΠ΅Π·Π΅Π½Ρ
ΠΈΠΌΠ½ΡΡ
ΡΡΠ²ΠΎΠ»ΠΎΠ²ΡΡ
ΠΊΠ»Π΅ΡΠΎΠΊ ΠΈΠ· ΡΠΊΠ°Π½Π΅ΠΈΜ ΠΌΠΎΠ»ΠΎΡΠ½ΡΡ
ΠΆΠ΅Π»Π΅Π· ΠΈ ΡΠΊΠ°Π½ΠΈ ΠΎΠΏΡΡ
ΠΎΠ»ΠΈ ΠΌΠΎΠ»ΠΎΡΠ½ΠΎΠΈΜ ΠΆΠ΅Π»Π΅Π·Ρ ΡΡΠ΅Π±ΡΡΡ Π΄Π°Π»ΡΠ½Π΅ΠΈΜΡΠ΅Π³ΠΎ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΡ
The effects of material formulation and manufacturing process on mechanical and thermal properties of epoxy/clay nanocomposites
A holistic study was conducted to investigate the combined effect of three different pre-mixing processes, namely mechanical mixing, ultrasonication and centrifugation, on mechanical and thermal properties of epoxy/clay nanocomposites reinforced with different platelet-like montmorillonite (MMT) clays (Cloisite Na+, Cloisite 10A, Cloisite 15 or Cloisite 93A) at clay contents of 3β10 wt%. Furthermore, the effect of combined pre-mixing processes and material formulation on clay dispersion and corresponding material properties of resulting composites was investigated using X-ray diffraction (XRD), transmission electron microscopy (TEM), scanning electron microscopy (SEM), flexural and Charpy impact tests, Rockwell hardness tests and differential scanning calorimetry (DSC). A high level of clay agglomeration and partially intercalated/exfoliated clay structures were observed regardless of clay type and content. Epoxy/clay nanocomposites demonstrate an overall noticeable improvement of up to 10 % in the glass transition temperature (Tg) compared to that of neat epoxy, which is interpreted by the inclusion of MMT clays acting as rigid fillers to restrict the chain mobility of epoxy matrices. The impact strength of epoxy/clay nanocomposites was also found to increase by up to 24 % with the addition of 3 wt% Cloisite Na+ clays. However, their flexural strength and hardness diminished when compared to those of neat epoxy, arising from several effects including clay agglomeration, widely distributed microvoids and microcracks as well as weak interfacial bonding between clay particles and epoxy matrices, as confirmed from TEM and SEM results. Overall, it is suggested that an improved technique should be used for the combination of pre-mixing processes in order to achieve the optimal manufacturing condition of uniform clay dispersion and minimal void contents
Transient Ureteral Obstruction Prevents against Kidney Ischemia/Reperfusion Injury via Hypoxia-Inducible Factor (HIF)-2Ξ± Activation
Although the protective effect of transient ureteral obstruction (UO) prior to ischemia on subsequent renal ischemia/reperfusion (I/R) injury has been documented, the underlying molecular mechanism remains to be understood. We showed in the current study that 24 h of UO led to renal tubular hypoxia in the ipsilateral kidney in mice, with the accumulation of hypoxia-inducible factor (HIF)-2Ξ±, which lasted for a week after the release of UO. To address the functions of HIF-2Ξ± in UO-mediated protection of renal IRI, we utilized the Mx-Cre/loxP recombination system to knock out target genes. Inactivation of HIF-2Ξ±, but not HIF-1Ξ± blunted the renal protective effects of UO, as demonstrated by much higher serum creatinine level and severer histological damage. UO failed to prevent postischemic neutrophil infiltration and apoptosis induction in HIF-2Ξ± knockout mice, which also diminished the postobstructive up-regulation of the protective molecule, heat shock protein (HSP)-27. The renal protective effects of UO were associated with the improvement of the postischemic recovery of intra-renal microvascular blood flow, which was also dependent on the activation of HIF-2Ξ±. Our results demonstrated that UO protected the kidney via activation of HIF-2Ξ±, which reduced tubular damages via preservation of adequate renal microvascular perfusion after ischemia. Thus, preconditional HIF-2Ξ± activation might serve as a novel therapeutic strategy for the treatment of ischemic acute renal failure
Recent trends in the use of electrical neuromodulation in Parkinson's disease
Purpose of Review: This review aims to survey recent trends in electrical forms of neuromodulation, with a specific application to Parkinsonβs disease (PD). Emerging trends are identified, highlighting synergies in state-of-the-art neuromodulation strategies, with directions for future improvements in stimulation efficacy suggested.
Recent Findings: Deep brain stimulation remains the most common and effective form of electrical stimulation for the treatment of PD. Evidence suggests that transcranial direct current stimulation (tDCS) most likely impacts the motor symptoms of the disease, with the most prominent results relating to rehabilitation. However, utility is limited due to its weak effects and high variability, with medication state a key confound for efficacy level. Recent innovations in transcranial alternating current stimulation (tACS) offer new areas for investigation.
Summary: Our understanding of the mechanistic foundations of electrical current stimulation is advancing and as it does so, trends emerge which steer future clinical trials towards greater efficacy