11 research outputs found
Myokines as a promising marker of metabolic disorders and physical activity
Currently, about 82 myokines identified and their number is increasing. It is shown that the major regulator of myokine expression and production is exercise. The expression level of IL-6 is dependent on the amount of muscle mass involved in contraction. It is assumed that the decrease in the partial pressure of oxygen, the increase in [Ca2+]i ratio and AMP/ATP (exercise response) are major regulator of transcriptome and proteome changes in the skeletal muscle cells, including a myokine set
Changes in the plasma levels of myokines after different physical exercises in athletes and untrained individuals
The influence of dynamic and static load on the plasma level of myokines in strength-and endurance-trained athletes and untrained subjects has been studied. The range of myokines has been found to depend on the type of loads and the level of fitness. Dynamic and static exercises have different effects on the level of myokines in athletes and untrained subjects. The dynamic load increases the level of IL-6 and IL-8 in the plasma of athletes, while the static load increases the concentration of IL-15 and LIF. At the same time, no increase in the level of IL-8 after cyclic loading or in IL-15 after a static load has been observed in the control group. These differences may be based on a number of mechanisms. The cellular composition of skeletal muscles and the phenotypic features of muscle fibers, changing as a result of regular exercise, can modify the processes of myokine production. However, the processes of transcription in muscle fibers are much more important; the most important ones are HIF-1Ξ±, [Ca2+]i and [Na+]i/[K+]i-dependent intracellular signaling pathways. The modification of these mechanisms caused by different physical loads and intensity is of great interest since it is a promising way to influence the metabolic processes at the cellular and systemic levels, which is very helpful in both improving athletic performance and correcting metabolic disorders in a number of socially significant diseases
Myokines as a promising marker of metabolic disorders and physical activity
Currently, about 82 myokines identified and their number is increasing. It is shown that the major regulator of myokine expression and production is exercise. The expression level of IL-6 is dependent on the amount of muscle mass involved in contraction. It is assumed that the decrease in the partial pressure of oxygen, the increase in [Ca2+]i ratio and AMP/ATP (exercise response) are major regulator of transcriptome and proteome changes in the skeletal muscle cells, including a myokine set
ΠΠ»ΠΈΡΠ½ΠΈΠ΅ ΡΡΠΎΠ²Π½Ρ Π΄Π²ΠΈΠ³Π°ΡΠ΅Π»ΡΠ½ΠΎΠΉ Π°ΠΊΡΠΈΠ²Π½ΠΎΡΡΠΈ Π½Π° ΠΏΡΠΎΡΡΡΠ°Π½ΡΡΠ²Π΅Π½Π½ΠΎΠ΅ ΡΠ°ΡΠΏΡΠ΅Π΄Π΅Π»Π΅Π½ΠΈΠ΅ Π°Π»ΡΡΠ°-ΡΠΈΡΠΌΠ° ΡΠ»Π΅ΠΊΡΡΠΎΡΠ½ΡΠ΅ΡΠ°Π»ΠΎΠ³ΡΠ°ΠΌΠΌΡ
ΠΠ½ΡΠ΅Π½ΡΠΈΠ²Π½ΠΎΡΡΡ ΠΈ Ρ
Π°ΡΠ°ΠΊΡΠ΅Ρ Π΄Π²ΠΈΠ³Π°ΡΠ΅Π»ΡΠ½ΠΎΠΉ Π°ΠΊΡΠΈΠ²Π½ΠΎΡΡΠΈ ΠΎΠΊΠ°Π·ΡΠ²Π°ΡΡ Π²Π»ΠΈΡΠ½ΠΈΠ΅ Π½Π° Π·Π°ΠΊΠΎΠ½ΠΎΠΌΠ΅ΡΠ½ΠΎΡΡΠΈ ΡΠΎΡΠΌΠΈΡΠΎΠ²Π°Π½ΠΈΡ ΠΏΠ°ΡΡΠ΅ΡΠ½ΠΎΠ² Π°Π»ΡΡΠ°-Π°ΠΊΡΠΈΠ²Π½ΠΎΡΡΠΈ ΠΊΠΎΡΡ ΠΏΠΎΠ»ΡΡΠ°ΡΠΈΠΉ Π³ΠΎΠ»ΠΎΠ²Π½ΠΎΠ³ΠΎ ΠΌΠΎΠ·Π³Π°. ΠΡΡΠ²Π»Π΅Π½Ρ ΠΎΡΠ»ΠΈΡΠΈΡ Π² ΡΡΠ½ΠΊΡΠΈΠΎΠ½Π°Π»ΡΠ½ΠΎΠΉ ΠΌΠ΅ΠΆΠΏΠΎΠ»ΡΡΠ°ΡΠ½ΠΎΠΉ Π°ΡΠΈΠΌΠΌΠ΅ΡΡΠΈΠΈ Π³ΠΎΠ»ΠΎΠ²Π½ΠΎΠ³ΠΎ ΠΌΠΎΠ·Π³Π° Ρ Π»ΠΈΡ Ρ Π½ΠΈΠ·ΠΊΠΈΠΌ ΡΡΠΎΠ²Π½Π΅ΠΌ Π΄Π²ΠΈΠ³Π°ΡΠ΅Π»ΡΠ½ΠΎΠΉ Π°ΠΊΡΠΈΠ²Π½ΠΎΡΡΠΈ. ΠΠΎΠΊΠ°Π·Π°Π½Ρ ΠΈΠ·ΠΌΠ΅Π½Π΅Π½ΠΈΡ ΡΡΠ½ΠΊΡΠΈΠΎΠ½Π°Π»ΡΠ½ΠΎΠΉ ΠΏΠΎΠ΄Π²ΠΈΠΆΠ½ΠΎΡΡΠΈ Π² Π³ΡΡΠΏΠΏΠ°Ρ
ΡΠΎ ΡΡΠ΅Π΄Π½ΠΈΠΌ ΠΈ Π²ΡΡΠΎΠΊΠΈΠΌ (Ρ ΠΏΡΠ΅ΠΎΠ±Π»Π°Π΄Π°Π½ΠΈΠ΅ΠΌ Π½Π°Π³ΡΡΠ·ΠΎΠΊ Π΄ΠΈΠ½Π°ΠΌΠΈΡΠ΅ΡΠΊΠΎΠ³ΠΎ Ρ
Π°ΡΠ°ΠΊΡΠ΅ΡΠ°) ΡΡΠΎΠ²Π½ΡΠΌΠΈ Π΄Π²ΠΈΠ³Π°ΡΠ΅Π»ΡΠ½ΠΎΠΉ Π°ΠΊΡΠΈΠ²Π½ΠΎΡΡΠΈ
ΠΠ»ΠΈΡΠ½ΠΈΠ΅ ΡΡΠΎΠ²Π½Ρ Π΄Π²ΠΈΠ³Π°ΡΠ΅Π»ΡΠ½ΠΎΠΉ Π°ΠΊΡΠΈΠ²Π½ΠΎΡΡΠΈ Π½Π° ΠΏΡΠΎΡΡΡΠ°Π½ΡΡΠ²Π΅Π½Π½ΠΎΠ΅ ΡΠ°ΡΠΏΡΠ΅Π΄Π΅Π»Π΅Π½ΠΈΠ΅ Π°Π»ΡΡΠ°-ΡΠΈΡΠΌΠ° ΡΠ»Π΅ΠΊΡΡΠΎΡΠ½ΡΠ΅ΡΠ°Π»ΠΎΠ³ΡΠ°ΠΌΠΌΡ
ΠΠ½ΡΠ΅Π½ΡΠΈΠ²Π½ΠΎΡΡΡ ΠΈ Ρ
Π°ΡΠ°ΠΊΡΠ΅Ρ Π΄Π²ΠΈΠ³Π°ΡΠ΅Π»ΡΠ½ΠΎΠΉ Π°ΠΊΡΠΈΠ²Π½ΠΎΡΡΠΈ ΠΎΠΊΠ°Π·ΡΠ²Π°ΡΡ Π²Π»ΠΈΡΠ½ΠΈΠ΅ Π½Π° Π·Π°ΠΊΠΎΠ½ΠΎΠΌΠ΅ΡΠ½ΠΎΡΡΠΈ ΡΠΎΡΠΌΠΈΡΠΎΠ²Π°Π½ΠΈΡ ΠΏΠ°ΡΡΠ΅ΡΠ½ΠΎΠ² Π°Π»ΡΡΠ°-Π°ΠΊΡΠΈΠ²Π½ΠΎΡΡΠΈ ΠΊΠΎΡΡ ΠΏΠΎΠ»ΡΡΠ°ΡΠΈΠΉ Π³ΠΎΠ»ΠΎΠ²Π½ΠΎΠ³ΠΎ ΠΌΠΎΠ·Π³Π°. ΠΡΡΠ²Π»Π΅Π½Ρ ΠΎΡΠ»ΠΈΡΠΈΡ Π² ΡΡΠ½ΠΊΡΠΈΠΎΠ½Π°Π»ΡΠ½ΠΎΠΉ ΠΌΠ΅ΠΆΠΏΠΎΠ»ΡΡΠ°ΡΠ½ΠΎΠΉ Π°ΡΠΈΠΌΠΌΠ΅ΡΡΠΈΠΈ Π³ΠΎΠ»ΠΎΠ²Π½ΠΎΠ³ΠΎ ΠΌΠΎΠ·Π³Π° Ρ Π»ΠΈΡ Ρ Π½ΠΈΠ·ΠΊΠΈΠΌ ΡΡΠΎΠ²Π½Π΅ΠΌ Π΄Π²ΠΈΠ³Π°ΡΠ΅Π»ΡΠ½ΠΎΠΉ Π°ΠΊΡΠΈΠ²Π½ΠΎΡΡΠΈ. ΠΠΎΠΊΠ°Π·Π°Π½Ρ ΠΈΠ·ΠΌΠ΅Π½Π΅Π½ΠΈΡ ΡΡΠ½ΠΊΡΠΈΠΎΠ½Π°Π»ΡΠ½ΠΎΠΉ ΠΏΠΎΠ΄Π²ΠΈΠΆΠ½ΠΎΡΡΠΈ Π² Π³ΡΡΠΏΠΏΠ°Ρ
ΡΠΎ ΡΡΠ΅Π΄Π½ΠΈΠΌ ΠΈ Π²ΡΡΠΎΠΊΠΈΠΌ (Ρ ΠΏΡΠ΅ΠΎΠ±Π»Π°Π΄Π°Π½ΠΈΠ΅ΠΌ Π½Π°Π³ΡΡΠ·ΠΎΠΊ Π΄ΠΈΠ½Π°ΠΌΠΈΡΠ΅ΡΠΊΠΎΠ³ΠΎ Ρ
Π°ΡΠ°ΠΊΡΠ΅ΡΠ°) ΡΡΠΎΠ²Π½ΡΠΌΠΈ Π΄Π²ΠΈΠ³Π°ΡΠ΅Π»ΡΠ½ΠΎΠΉ Π°ΠΊΡΠΈΠ²Π½ΠΎΡΡΠΈ
Dynamic and static exercises differentially affect plasma cytokine content in elite endurance- and strength-trained athletes and untrained volunteers
Extensive exercise increases the plasma content of IL-6, IL-8, IL-15, leukemia inhibitory factor (LIF), and several other cytokines via their augmented transcription in skeletal muscle cells. However, the relative impact of aerobic and resistant training interventions on cytokine production remains poorly defined. In this study, we compared effects of dynamic and static load on cytokine plasma content in elite strength- and endurance-trained athletes vs. healthy untrained volunteers. The plasma cytokine content was measured before, immediately after, and 30 min post-exercise using enzyme-linked immunosorbent assay. Pedaling on a bicycle ergometer increased IL-6 and IL-8 content in the plasma of trained athletes by about 4- and 2-fold, respectively. In contrast to dynamic load, weightlifting had negligible impact on these parameters in strength exercise-trained athletes. Unlike IL-6 and IL-8, dynamic exercise had no impact on IL-15 and LIF, whereas static load increases the content of these cytokines by ~50%. Two-fold increment of IL-8 content seen in athletes subjected to dynamic exercise was absent in untrained individuals, whereas the ~50% increase in IL-15 triggered by static load in the plasma of weightlifting athletes was not registered in the control group. Thus, our results show the distinct impact of static and dynamic exercises on cytokine content in the plasma of trained athletes. They also demonstrate that both types of exercises differentially affect cytokine content in plasma of athletes and untrained persons
Level of interleukins IL-6 and IL-15 in blood plasma of mice after forced swimming test
We measured the concentrations of IL-6 and IL-15 in blood plasma of mice at different terms after forced swimming, taking into account exercise intensity and preliminary training. It was shown that training was an important factor affecting blood plasma level of IL both at rest and after single forced swimming: in trained animals, the concentration of both myokines increased immediately after swimming, while in untrained animals, this increase was observed only after 5 h. Changes in cytokine production against the background of training can be associated with various factors, including neuroendocrine mechanisms, stress, modification of intracellular signaling, as well as reorganization of transcriptional mechanisms in muscle fibers. The most important factor is shift in the ratio of monovalent cations (sodium and potassium) in the cytoplasm
Level of interleukins IL-6 and IL-15 in blood plasma of mice after forced swimming test
We measured the concentrations of IL-6 and IL-15 in blood plasma of mice at different terms after forced swimming, taking into account exercise intensity and preliminary training. It was shown that training was an important factor affecting blood plasma level of IL both at rest and after single forced swimming: in trained animals, the concentration of both myokines increased immediately after swimming, while in untrained animals, this increase was observed only after 5 h. Changes in cytokine production against the background of training can be associated with various factors, including neuroendocrine mechanisms, stress, modification of intracellular signaling, as well as reorganization of transcriptional mechanisms in muscle fibers. The most important factor is shift in the ratio of monovalent cations (sodium and potassium) in the cytoplasm