16 research outputs found
Separation of small molecular peptides with the same amino acid composition but different sequences by high performance liquid chromatography-electrospray ionization-mass spectrometry
Morphology, palaeoecology and phylogenetic interpretation of the Cambrian echinoderm Vyscystis
Cyclodextrin modified gold nanoparticles-based open-tubular capillary electrochromatographic separations of polyaromatic hydrocarbons
On-line immunoaffinity solid-phase extraction capillary electrophoresis-mass spectrometry for the analysis of serum transthyretin
The analysis of low abundant proteins in biological fluids by capillary electrophoresis (CE) is particularly problematic due to the typically poor concentration limits of detection of microscale separation techniques. Another important issue is sample matrix complexity that requires an appropriate cleanup. Here, we describe an on-line immunoaffinity solid-phase extraction capillary electrophoresis-mass spectrometry (IA-SPE-CE-MS) method for the immunoextraction, preconcentration, separation, detection, and characterization of serum transthyretin (TTR). TTR is a protein biomarker related to diverse types of amyloidosis, such as familial amyloidotic polyneuropathy type I (FAP-I), which is the most common hereditary systemic amyloidosis