3,176 research outputs found
Polymorphic microsatellite loci from the western corn rootworm (Insecta: Coleoptera: Chrysomelidae) and cross-amplification with other Diabrotica spp
Corn rootworms (Diabrotica spp.) make up the major insect pest complex of corn in the US and Europe, and there is a need for molecular markers for genetics studies. We used an enrichment strategy to develop microsatellite markers from the western corn rootworm (Diabrotica virgifera virgifera). Of 54 loci isolated, 25 were polymorphic, and of these, 17 were surveyed for variability in 59 wild individuals. In addition, the potential for cross-amplification of these microsatellites was surveyed for Mexican, northern, and southern corn rootworms. Nine microsatellite loci showed Mendelian inheritance and are likely to be useful in population genetics studies
Cell-type specific potent Wnt signaling blockade by bispecific antibody.
Cell signaling pathways are often shared between normal and diseased cells. How to achieve cell type-specific, potent inhibition of signaling pathways is a major challenge with implications for therapeutic development. Using the Wnt/β-catenin signaling pathway as a model system, we report here a novel and generally applicable method to achieve cell type-selective signaling blockade. We constructed a bispecific antibody targeting the Wnt co-receptor LRP6 (the effector antigen) and a cell type-associated antigen (the guide antigen) that provides the targeting specificity. We found that the bispecific antibody inhibits Wnt-induced reporter activities with over one hundred-fold enhancement in potency, and in a cell type-selective manner. Potency enhancement is dependent on the expression level of the guide antigen on the target cell surface and the apparent affinity of the anti-guide antibody. Both internalizing and non-internalizing guide antigens can be used, with internalizing bispecific antibody being able to block signaling by all ligands binding to the target receptor due to its removal from the cell surface. It is thus feasible to develop bispecific-based therapeutic strategies that potently and selectively inhibit signaling pathways in a cell type-selective manner, creating opportunity for therapeutic targeting
N 1,N 4-Bis(2-thienylmethylÂene)cycloÂhexane-1,4-diamine
The title compound, C16H18N2S2, lies about an inversion center with only half of the molÂecule in the asymmetric unit. The cycloÂhexane ring adopts a chair conformation, and the terminal thioÂphene rings are in a transoid orientation, with an S⋯S separation between the two terminal 2-thioÂphene rings of 11.6733 (9) Å
Induced local spin-singlet amplitude and pseudogap in high cuprates
In this paper we show that local spin-singlet amplitude with d-wave symmetry,
, can be induced by short-range spin correlations even
in the absence of pairing interactions. Fluctuation theory is formulated to
make connection between pseudogap temperature $T^{*}$, pseudogap size
$\Delta_{pg}$ and . In the present scenario for the
pseudogap, the normal state pseudogap is caused by the induced local
spin-singlet amplitude due to short-range spin correlations, which compete in
the low energy sector with superconducting correlations to make go to
zero near half-filling. Calculated falls from a high value onto the
line and closely follows mean-field N\'{e}el temperature .
The calculated is in good agreement with experimental results. We
propose an experiment in which the present scenario can be critically tested.Comment: 5 pages, 3 figure
Charge Transfer Induced Molecular Hole Doping into Thin Film of Metal-Organic-Frameworks
Despite the highly porous nature with significantly large surface area, metal
organic frameworks (MOFs) can be hardly used in electronic, and optoelectronic
devices due to their extremely poor electrical conductivity. Therefore, the
study of MOF thin films that require electron transport or conductivity in
combination with the everlasting porosity is highly desirable. In the present
work, thin films of Co3(NDC)3DMF4 MOFs with improved electronic conductivity
are synthesized using layer-by-layer and doctor blade coating techniques
followed by iodine doping. The as-prepared and doped films are characterized
using FE-SEM, EDX, UV/Visible spectroscopy, XPS, current-voltage measurement,
photoluminescence spectroscopy, cyclic voltammetry, and incident photon to
current efficiency measurements. In addition, the electronic and semiconductor
property of the MOF films are characterized using Hall Effect measurement,
which reveals that in contrast to the insulator behavior of the as-prepared
MOFs, the iodine doped MOFs behave as a p-type semiconductor. This is caused by
charge transfer induced hole doping into the frameworks. The observed charge
transfer induced hole doping phenomenon is also confirmed by calculating the
densities of states of the as-prepared and iodine doped MOFs based on density
functional theory. Photoluminescence spectroscopy demonstrate an efficient
interfacial charge transfer between TiO2 and iodine doped MOFs, which can be
applied to harvest solar radiations.Comment: Main paper (19 pages, 6 figures) and supplementary information (15
pages, 10 figures), accepted in ACS Appl. Materials & Interface
Quantum Monte Carlo Study of Strongly Correlated Electrons: Cellular Dynamical Mean-Field Theory
We study the Hubbard model using the Cellular Dynamical Mean-Field Theory
(CDMFT) with quantum Monte Carlo (QMC) simulations. We present the algorithmic
details of CDMFT with the Hirsch-Fye QMC method for the solution of the
self-consistently embedded quantum cluster problem. We use the one- and
two-dimensional half-filled Hubbard model to gauge the performance of CDMFT+QMC
particularly for small clusters by comparing with the exact results and also
with other quantum cluster methods. We calculate single-particle Green's
functions and self-energies on small clusters to study their size dependence in
one- and two-dimensions.Comment: 14 pages, 18 figure
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