IDENTIFIKASI LARVA LALAT DALAM KEPENTINGAN POST MORTEM INTERVAL PADA BANGKAI TIKUS (Rattus novergicus) YANG DIBERI CIU OPLOSAN DI SCIENCE TECHNO PARK UNIVERSITAS MUHAMMADIYAH PURWOKERTO

Ciu merupakan salah satu minuman tradisional Banyumas yang mengandung alkohol. Ciu dapat dibuat oplosan dengan bahan lain untuk meningkatkan cita rasa dan sensasi minuman, namun berdampak buruk bagi kesehatan bahkan dapat menyebabkan kematian. Serangga berperan penting dalam penentuan Post Mortem Interval. Adanya kandungan obat atau senyawa yang terdapat pada jenazah dapat mempengaruhi perkembangan larva lalat. Tujuan penelitian ini untuk mengetahui spesies larva lalat yang terdapat pada bangkai tikus yang diberi ciu oplosan. Penelitian dilakukan secara observasional deskriptif. Penelitian dilakukan pada bulan Juli-Agustus 2018.Sebanyak tiga ekor tikus Wistar diberi ciu yang dioplos dengan minuman berenergi, ditunggu hingga mati kemudian diletakkan di Science Techno Park Universitas Muhammadiyah Purwokerto. Larva yang diambil 10% dari total populasi yang ditemukan setiap harinya. Identifikasi larva melalui pembuatan preparat posterior spirakel. Data dianalisis dengan univariate. Larva Calliphora sp. (19,10%), Sarcophaga sp. (0,71%), Chrysomya megacephala (13,28%), dan Chrysomya bezziana (13,86%) teridentifikasi pada bangkai tikus yang diberi ciu oplosan di Science Techno Park Universitas Muhammadiyah Purwokerto. Kata Kunci: Ciu, Oplosan, Post Mortem Interval, Larva Lala

Kadar IgE Penderita Alergi pada Mahasiswa Teknologi Laboratorium Medik Universitas Muhammadiyah Purwokerto

The prevalence of allergies is increasing from year to year due to the modern lifestyle in society. The incidence of allergies was influenced by several factors, such as genetics, age, sex, diet, food type, and also environmental factors. Determination of IgE levels in adolescents and young adults is important because the incidence of allergy is highly reported in adolescents and young adults. This study was conducted to determine IgE levels in allergies among university students in Universitas Muhammadiyah Purwokerto. This observational study was conducted among diploma students of Medical Laboratory Technology, Universitas Muhammadiyah Purwokerto. The data of characteristic subjects were collected by a self-administered questionnaire whereas data of IgE levels were collected by serum samples and measured by ELISA. A total of 23 students (4.35 % male and 95.65% female) were included. Seafood allergy (25%) were the commonest allergy among the students. Only 9% of subjects have combination allergies. The highest of IgE levels were 365 IU/mL

Determination Of IgE Levels In Allergies Among Medical Laboratory Technology Students In Universitas Muhammadiyah Purwokerto

The prevalence of allergies is increasing from year to year due to the modern lifestyle in society. The incidence of allergies was influenced by several factors, such as genetics, age, sex, diet, food type, and also environmental factors. Determination Ig E levels in adolescents and young adults is important because the incidence of allergy is highly reported in adolescents and young adults. This study was conducted to determine Ig E levels in allergies among university students in Universitas Muhammadiyah Purwokerto This observational study was conducted among diploma students of Medical Laboratory Technology, Universitas Muhammadiyah Purwokerto. The data of characteristic subjects were collected by a self-administered questionnaire whereas data of Ig E levels were collected by serum samples and measured by ELISA. A total of 23 students (4,35 % male and 95,65% female) were included. Seafood allergy (25%) were the commonest allergy among the students. Only 9% of subjects have combination allergies. The highest of Ig E levels were 365 IU/mL

The Role of Body Mass Index, Hemogblobin Levels in Women Giving Birth to Neonatal Weight

Maternal nutritional status is monitored with BMI and is also one of the factors that have an impact during pregnancy. Poor nutrition before and during pregnancy leads to decreasing hemoglobin levels and low birth weight infants. This study aims to determine the correlation between hemoglobin levels and BMI of mothers giving birth to the weight of newborns at the Puskesmas Kebumen II, Kebumen District. This research used a retrospective study with medical record data collected from January to April 2022. This research involved 50 patient mothers giving birth at Puskesmas Kebumen II, Kebumen District. The data were analyzed by the Pearson correlation coefficient test. The results are BMI and newborn weight (p = 0.061, r = 0.367), hemoglobin levels, and newborn weight (p=0.852; r= -0.025). There was no correlation between BMI and hemoglobin levels to newborn weight at Puskemas Kebumen II, Kebumen District.Maternal nutritional status is monitored with BMI and is also one of the factors that have an impact during pregnancy. Poor nutrition before and during pregnancy leads to decreasing hemoglobin levels and low birth weight infants. This study aims to determine the correlation between hemoglobin levels and BMI of mothers giving birth to the weight of newborns at the Puskesmas Kebumen II, Kebumen District. This research used a retrospective study with medical record data collected from January to April 2022. This research involved 50 patient mothers giving birth at Puskesmas Kebumen II, Kebumen District. The data were analyzed by the Pearson correlation coefficient test. The results are BMI and newborn weight (p = 0.061, r = 0.367), hemoglobin levels, and newborn weight (p=0.852; r= -0.025). There was no correlation between BMI and hemoglobin levels to newborn weight at Puskemas Kebumen II, Kebumen District

Efektivitas Kombinasi Ekstrak Lidah Buaya dan Lidah Mertua Terhadap Mortalitas Larva Aedes aegypti

The Dengue Haemoragic Fever (DHF) vector control with synthetic larvicide has an impact on health and environmental problems, so alternative plant-based insecticides are needed. The study aims to determine the larvicidal effect of a combination with Aloe vera extract and Sansivieria trifasciata on the mortality of Aedes aegypti larvae. This study was conducted with experimental design by a post-test only with control groups design consisting of five groups. Two groups are positive control (1% temephos) and negative control (0% concentration). The other three groups served as a combination of Aloe vera extract and S. trifasciata Prain in ratio of 75%:25% (P1), 50%:50% (P2), and 25%:75% (P3). A total of 375 larvae were introduced in this study with three replications and with observation time of 30 minutes, 1 hour, 3 hours, 6 hours, 12 hours, 24 hours until totally died 100%. The mortality of Aedes aegypti larvae were analyzed by Kruskal Wallis and probit analysis to calculate LC50 and LC90. The larvae totally died 100% after 12 hours of exposure. There are significant differences in the mortality of Ae. aegypti larvae after administration of a combination with Aloe vera extract and S. trifasciata Prain. The P1 group (The combination with Aloe vera extract and S. trifasciata Prain with a ratio of 75%:25%) was the most effective in mortality of Ae. aegypti larvae. The LC50 value is 0.517% while the LC90 value is 2.121% within 6 hours. The combination group of Aloe vera extract and mother-in-law's tongue with a ratio of 75%:25% was the most effective in causing mortality of Ae. aegypti within 6 hours

Deteksi Endoparasit Cacing pada Hepar Tikus Laboratorium (Rattus norvegicus) dari Sentra Peternak di Kabupaten Banyumas dan Kabupaten Purbalingga

The presence of laboratory rats that are maintained and bred for laboratory purposes or laboratory observations is very necessary. The presence of endoparasite in laboratory rats will have an impact on the result of the research or laboratory observations. This study aims to detect helminth endoparasites in the liver of laboratory rats (Rattus norvegicus) from animal breeders in Banyumas and Purbalingga Districts. This research was an observational study with a cross-sectional design. A total of 52 laboratory rats were used in the study. Rats are killed with chloroform, liver surgery then identify the presence of worm larvae. Out of the 52 rats obtained, 7 (29.17%) from 24 laboratory rats in Banyumas District and 5 (17.86%) from 28 laboratory rats in Purbalingga District were infected with Taenia taeniaeformis. It is necessary to control helminth infections in laboratory rats, such as laboratory animal quarantine, health monitoring, and antihelmintic treatment. It is important to handle carefully during travel to assure the results of research or laboratory observations using the animals

Kombinasi Minyak Kayu Putih dan Jeruk Nipis terhadap Mortalitas Pediculus humanus capitis

The use of synthetic chemical pediculicides is known to have side effects and the occurrence of resistance so plant-based pediculicides are needed to kill Ph. capitis. Eucalyptus and lime oil are known to have an effect in killing Ph. capitis. The purpose of this study was to determine the effectiveness of the combination of eucalyptus and lime oil on the mortality of Ph. capitis. This study was conducted in a true experimental with a post-test-only control group design in May 2021. This study consisted of 5 treatment groups, one group as a positive control (permethrin 1%), one group as a negative control (aquadest), and 3 groups received treatment with a combination of eucalyptus and lime oil in the proportions of 1:3 (25 ml of eucalyptus oil: 75 ml of lime juice), 1:1 (50 ml of eucalyptus oil: 50 ml of lime juice) and 3: 1 (75 ml eucalyptus oil: 25 ml lime juice). It was repeated 3 times so that the total sample was 150 Ph. capitis which was observed every 5 minutes for 1 hour. The research data were analyzed using the Kruskal-Wallis test and continued with the Posthoc Mann-Whitney U test. Mortality of Ph. capitis in the combination group the most effective was the combination of 75% eucalyptus oil and 25% lime oil in the 5th-minute observation of 100% mortality compared to the control group and other groups

Efektivitas Ekstrak Daun Kemangi (Ocimum sanctum Linn) Terhadap Mortalitas Larva Aedes aegypti

Basil leaf (Ocimum sanctum Linn) are known to have the ability as natural larvicides because there are various compounds including tannins, eugenol, flavonoids, essential oils, hexauronic acid, saponins, pentose, xylose, metal homocytic acid, mulludistin, ursolic acid and methyl clavical. This study aims to determine the effectiveness of basil leaf extract (O. sanctum Linn) on mortality of Aedes agypti larvae. This research is a true experimental type with posttest only with control group design. Aedes aegypti larvae obtained from Balitbangkes Banjarnegara were used in this study as many as 450 larvae. This study consisted of 6 groups with 2 control groups (positive control/temefos 1% and negative control/distilled water) and 4 other groups receiving treatment with basil leaf extract concentrations of 1.5%, 1.8%, 2.1%, and 2.4%. Each group contained 25 larvae of Ae. aegypti with three repetitions and observation time with 10th, 40th, 60th, 120th, 240, 720, and 1440 minute intervals. Larval mortality of Ae. aegypti occurred from the 120th minute with a concentration of 2.4% with a mortality percentage of 5.6%. The results of the Kruskal Wallis test showed that there were differences in the mortality of Ae. aegypti at 60, 120, 240, 720, and 1440 minutes. The LC50 value was 2.329% at 720 minutes while the LC90 test was 7.043% at 720 minutes. Basil leaf extract has not been effective as a larvicide because mortality occurred from the 120th minute

EFEK MONOSODIUM GLUTAMAT TERHADAP GAMBARAN HISTOLOGI HEPAR TIKUS PUTIH (Rattus norvegicus) GALUR WISTAR

Monosodium glutamate (MSG) is a sodium salt of glutamic acid that naturally comes from vegetable and animal protein functioned as a taste enhancer. So far, the use of MSG in various countries has increased by 1 g / day and can have an impact on cell damage to several organs. The purpose of this study was to find out the effects of MSG on the liver histology of Wistar white rats (Rattus norvegicus). This study used Posttest Only Control Group Design consisting of 4 groups, as control and MSG treatment doses of 5 g / kg BW, 10 g / kg BW, and 15 g / kg BW for 14 days orally. Each group consisted of 6 male white rats (Rattus norvegicus) aged 2-3 months with a weight of 200-250 grams in good health. A day after 14 days of treatment, the rats' liver organs were taken and preparations were made using the paraffin method. Data were analyzed using the Kruskal-Wallis test. The results of research showed that the control group looked normal, while the P1 group had 40.5 (40-43)% cell abnormalities and 52.5 (27-56)% cell abnormalities. The P3 group showed the most significant liver cell damage, namely 60 (59-62)%. The results of the Kruskal-Wallis test analysis showed a significant difference of p = 0.034 between cell abnormalities and the dose level of MSG (p-value 0.05). It can be concluded that MSG administration in stratified doses can cause liver cell damage in the form of degeneration and necrosis. Giving a dose of MSG 15 g / kg BW for 14 days showed the most significant liver cell damage at 60 (59-62)%.Keywords: Cell Abnormality, Liver, Monosodium Glutamat