Power-to-Strength Ratio Influences Performance Enhancement with Contrast Training

The effectiveness of contrast training (CST) for improving explosive exercise performance is modulated by various individual characteristics; however, further work is required to define these factors.Subelite male Australian Football players (n = 22; age, 19 ± 2 yr; body mass, 80.4 ± 9.4 kg; one-repetition maximum [1-RM] half squat, 172 ± 18 kg; mean ± SD) completed two experimental trials involving two sets of squat jumps (six repetitions at 30% 1-RM) performed either alone (CTL condition) or after half squats (six repetitions at 85% 1-RM; CST condition).Squat jump peak power was similar between CTL and CST during set 1 (mean change: ±90% confidence interval, 2.8% ± 2.0%; effect size [ES]: ±90% confidence interval, 0.13 ± 0.09; P = 0.079) and set 2 (0.3% ± 1.7%; ES, 0.01 ± 0.08; P = 0.781). Peak power enhancement with CST was not related to maximal (1-RM half squat) strength (r = 0.001, P = 0.884), but was negatively correlated with both baseline peak power (r = 0.44, P \u3c 0.001) and power-to-strength ratio (PSR); that is, the ratio between baseline peak power and 1-RM half squat strength (r = 0.65, P \u3c 0.001). Using a median split, analyses were performed in participants with a low PSR (LPSR group; PSR = 15.4-19.1 W·kg; n = 11) or high PSR (HPSR group, PSR = 19.4-24.7 W·kg; n = 11). Peak power was enhanced with CST for the LPSR (8.1% ± 3.9%; ES, 0.44 ± 0.21; P = 0.004) but not HPSR (-2.1% ± 1.3%; ES, -0.14 ± 0.09; P = 0.010) groups.The PSR appears to influence the effectiveness of CST, with performance enhancement more likely in those with a lower PSR

The Mosaic Genome of Anaeromyxobacter dehalogenans Strain 2CP-C Suggests an Aerobic Common Ancestor to the Delta-Proteobacteria

©2008 Thomas et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.doi:10.1371/journal.pone.0002103Anaeromyxobacter dehalogenans strain 2CP-C is a versaphilic delta-Proteobacterium distributed throughout many diverse soil and sediment environments. 16S rRNA gene phylogenetic analysis groups A. dehalogenans together with the myxobacteria, which have distinguishing characteristics including strictly aerobic metabolism, sporulation, fruiting body formation, and surface motility. Analysis of the 5.01 Mb strain 2CP-C genome substantiated that this organism is a myxobacterium but shares genotypic traits with the anaerobic majority of the delta-Proteobacteria (i.e., the Desulfuromonadales). Reflective of its respiratory versatility, strain 2CP-C possesses 68 genes coding for putative c-type cytochromes, including one gene with 40 heme binding motifs. Consistent with its relatedness to the myxobacteria, surface motility was observed in strain 2CP-C and multiple types of motility genes are present, including 28 genes for gliding, adventurous (A-) motility and 17 genes for type IV pilus-based motility (i.e., social (S-) motility) that all have homologs in Myxococcus xanthus. Although A. dehalogenans shares many metabolic traits with the anaerobic majority of the delta- Proteobacteria, strain 2CP-C grows under microaerophilic conditions and possesses detoxification systems for reactive oxygen species. Accordingly, two gene clusters coding for NADH dehydrogenase subunits and two cytochrome oxidase gene clusters in strain 2CP-C are similar to those in M. xanthus. Remarkably, strain 2CP-C possesses a third NADH dehydrogenase gene cluster and a cytochrome cbb3 oxidase gene cluster, apparently acquired through ancient horizontal gene transfer from a strictly anaerobic green sulfur bacterium. The mosaic nature of the A. dehalogenans strain 2CP-C genome suggests that the metabolically versatile, anaerobic members of the delta-Proteobacteria may have descended from aerobic ancestors with complex lifestyles

The effects of caffeine ingestion on time trial cycling performance

Purpose:The purpose of this work was to determine the effects of caffeine on high intensity time trial (TT) cycling performance in well-trained subjects.Subjects:Six male cyclists with the following physical characteristics (mean ± SD) age 30.7 ± 12, height 179.3 ± 7.5 cm, mass 70.0 ± 7.5 kg, VO2max 65.0 ± 6.3 mL·kg−1·min−1 undertook three 1-h TT performances, control (C), placebo (P) and caffeine (CAF), on a Velotron cycle ergometer conducted in a double-blind, random fashion. Subjects rested for 60 min and were then given CAF or P in a dose of 6 mg·kg−1 body mass and then commenced exercise after another 60 min of rest. Before ingestion, 60 min postingestion, and at the end of the TT, finger-prick blood samples were analyzed for lactate.Results:The cyclists rode significantly further in the CAF trial (28.0 ± 1.3 km) than they did in the C (26.3 ± 1.5 km, P < .01) or P (26.4 ± 1.5 km, P < .02) trials. No differences were seen in heart rate data throughout the TT (P > .05). Blood lactate levels were significantly higher at the end of the trials than either at rest or postingestion (P < .0001), but there were no differences between the three trial groups.Conclusion:On the basis of the data, we concluded that performance was improved with the use of a caffeine supplement.Lars R. McNaughton, Ric J. Lovell, Jason Siegler, Adrian W. Midgley, Laura J.S. Moore and David J. Bentle

Effects of two pre-workout supplements on concentric and eccentric force production during lower body resistance exercise in males and females: a counterbalanced, double-blind, placebo-controlled trial

Abstract Background Pre-workout supplements purportedly enhance feelings of energy, reduce fatigue and improve exercise performance. The purpose of this study was to examine the performance effects of caffeinated and non-caffeinated multi-ingredient pre-workout supplements. Methods In a counterbalanced, double-blind, placebo-controlled design, eccentric and concentric force production during lower body resistance exercise on a mechanized squat device were assessed after supplement ingestion. Repetitions-in-reserve/RPE and subjective feelings of energy, focus and fatigue were also examined. Twenty-one resistance-trained adults (12 F, 9 M) completed three conditions in random order: caffeinated supplement, non-caffeinated supplement and placebo. Subjects were not informed of the presence of a placebo condition. Thirty minutes after supplement ingestion, a 3-repetition maximum test and 5 sets of 6 repetitions were completed using the squat device. Each repetition involved 4-s eccentric and concentric phases, and the force signal throughout each repetition was sampled from a load cell contained within the squat device. The scaled and filtered force signals were analyzed using customized software. Repeated measures analysis of variance and appropriate follow-up analyses were utilized to compare dependent variables, and relevant effect sizes (d) were calculated. Results Supplement or placebo ingestion led to similar subjective responses (p > 0.05). Energy (+8 to 44%; d = 0.3 to 0.8) and focus (+8 to 25%; d = 0.3 to 0.5) were acutely increased by supplement or placebo ingestion and decreased as the exercise session progressed. Fatigue was acutely decreased by supplement or placebo ingestion (−7 to 38%; d = −0.1 to −0.6) and increased as the exercise session progressed. Eccentric and concentric forces were unimproved by supplementation during the exercise sets for both sexes. In the non-caffeinated supplement condition only, maximal eccentric force production was lower during sets 3 to 5, as compared to set 1 (p < 0.05). Effect size data indicated that both the caffeinated and non-caffeinated supplements may contribute to small increases in concentric force production in males (+5 to 20%, d = 0.2 to 0.4 relative to placebo), but not females. Conclusions As compared to placebo, caffeinated and non-caffeinated multi-ingredient pre-workout supplements failed to improve concentric and eccentric force production. In males, effect size data indicate a possible small benefit of supplementation on concentric force production, although this was not statistically significant. When resistance-trained subjects were unaware of the presence of a placebo, resistance exercise performance was similar regardless of whether a placebo or multi-ingredient supplement was ingested