87 research outputs found
Aphid Wing Induction and Ecological Costs of Alarm Pheromone Emission under Field Conditions
The pea aphid, Acyrthosiphon pisum Harris, (Homoptera: Aphididae) releases the volatile sesquiterpene (E)-β-farnesene (EBF) when attacked by a predator, triggering escape responses in the aphid colony. Recently, it was shown that this alarm pheromone also mediates the production of the winged dispersal morph under laboratory conditions. The present work tested the wing-inducing effect of EBF under field conditions. Aphid colonies were exposed to two treatments (control and EBF) and tested in two different environmental conditions (field and laboratory). As in previous experiments aphids produced higher proportion of winged morphs among their offspring when exposed to EBF in the laboratory but even under field conditions the proportion of winged offspring was higher after EBF application (6.84±0.98%) compared to the hexane control (1.54±0.25%). In the field, the proportion of adult aphids found on the plant at the end of the experiment was lower in the EBF treatment (58.1±5.5%) than in the control (66.9±4.6%), in contrast to the climate chamber test where the numbers of adult aphids found on the plant at the end of the experiment were, in both treatments, similar to the numbers put on the plant initially. Our results show that the role of EBF in aphid wing induction is also apparent under field conditions and they may indicate a potential cost of EBF emission. They also emphasize the importance of investigating the ecological role of induced defences under field conditions
Research on the Stability of a Rabbit Dry Eye Model Induced by Topical Application of the Preservative Benzalkonium Chloride
Dry eye is a common disease worldwide, and animal models are critical for the study of it. At present, there is no research about the stability of the extant animal models, which may have negative implications for previous dry eye studies. In this study, we observed the stability of a rabbit dry eye model induced by the topical benzalkonium chloride (BAC) and determined the valid time of this model.). Decreased levels of mucin-5 subtype AC (MUC5AC), along with histopathological and ultrastructural disorders of the cornea and conjunctiva could be observed in Group BAC-W4 and particularly in Group BAC-W5 until day 21.A stable rabbit dry eye model was induced by topical 0.1% BAC for 5 weeks, and after BAC removal, the signs of dry eye were sustained for 2 weeks (for the mixed type of dry eye) or for at least 3 weeks (for mucin-deficient dry eye)
Evolution of active galactic nuclei
[Abriged] Supermassive black holes (SMBH) lurk in the nuclei of most massive
galaxies, perhaps in all of them. The tight observed scaling relations between
SMBH masses and structural properties of their host spheroids likely indicate
that the processes fostering the growth of both components are physically
linked, despite the many orders of magnitude difference in their physical size.
This chapter discusses how we constrain the evolution of SMBH, probed by their
actively growing phases, when they shine as active galactic nuclei (AGN) with
luminosities often in excess of that of the entire stellar population of their
host galaxies. Following loosely the chronological developments of the field,
we begin by discussing early evolutionary studies, when AGN represented beacons
of light probing the most distant reaches of the universe and were used as
tracers of the large scale structure. This early study turned into AGN
"Demography", once it was realized that the strong evolution (in luminosity,
number density) of the AGN population hindered any attempt to derive
cosmological parameters from AGN observations directly. Following a discussion
of the state of the art in the study of AGN luminosity functions, we move on to
discuss the "modern" view of AGN evolution, one in which a bigger emphasis is
given to the physical relationships between the population of growing black
holes and their environment. This includes observational and theoretical
efforts aimed at constraining and understanding the evolution of scaling
relations, as well as the resulting limits on the evolution of the SMBH mass
function. Physical models of AGN feedback and the ongoing efforts to isolate
them observationally are discussed next. Finally, we touch upon the problem of
when and how the first black holes formed and the role of black holes in the
high-redshift universe.Comment: 75 pages, 35 figures. Modified version of the chapter accepted to
appear in "Planets, Stars and Stellar Systems", vol 6, ed W. Keel
(www.springer.com/astronomy/book/978-90-481-8818-5). The number of references
is limited upon request of the editors. Original submission to Springer: June
201
Hydrogel-based scaffolds to support intrathecal stem cell transplantation as a gateway to the spinal cord: clinical needs, biomaterials, and imaging technologies
The prospects for cell replacement in spinal cord diseases are impeded by inefficient stem
cell delivery. The deep location of the spinal cord and complex surgical access, as well as
densely packed vital structures, question the feasibility of the widespread use of multiple
spinal cord punctures to inject stem cells. Disorders characterized by disseminated
pathology are particularly appealing for the distribution of cells globally throughout the
spinal cord in a minimally invasive fashion. The intrathecal space, with access to a
relatively large surface area along the spinal cord, is an attractive route for global stem cell
delivery, and, indeed, is highly promising, but the success of this approach relies on the
ability of cells 1) to survive in the cerebrospinal fluid (CSF), 2) to adhere to the spinal cord
surface, and 3) to migrate, ultimately, into the parenchyma. Intrathecal infusion of cell
suspension, however, has been insufficient and we postulate that embedding
transplanted cells within hydrogel scaffolds will facilitate reaching these goals. In this
review, we focus on practical considerations that render the intrathecal approach clinically
viable, and then discuss the characteristics of various biomaterials that are suitable to
serve as scaffolds. We also propose strategies to modulate the local microenvironment
with nanoparticle carriers to improve the functionality of cellular grafts. Finally, we
provide an overview of imaging modalities for in vivo monitoring and characterization of
biomaterials and stem cells. This comprehensive review should serve as a guide for those
planning pre-clinical and clinical studies on intrathecal stem cell transplantation.Funds provided under the project NanoTech4ALS (ref. ENMed/0008/2015, 13/EuroNanoMed/2016), funded under the EU FP7 M-ERA.NET program, Strategmed 1/233209/12/NCBIR/2015, and NIH R01 NS091100. The FCT distinction attributed to J.M.O. under the Investigator FCT program (IF/01285/2015) is also gratefully acknowledgedinfo:eu-repo/semantics/publishedVersio
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