Genetic diversity analysis of the medicinal herb Plantago ovata (Forsk.)

Plantago ovata (Forsk.) (2n = 8) used as laxative, emollient and demulcent, has great commercial and medicinal importance. With India being the largest producer in the world there is still a lack of defined varieties of the species and no coordinated breeding efforts are being made. In the present study, we report the phylogenetic analysis of the crop for its utilization in future breeding programs for defining varieties of the crop. A total of 302 clear and reproducible bands were obtained with random amplified polymorphic DNA (RAPD) techniques involving 35 random primers in 18 selected lines, out of which 198 (65.5%) were polymorphic with an average 8.6 bands per primer. Amplified DNA fragments ranged from 300 to 3400 bp. Dissimilarity indices based on Nei and Li equation ranged from 0.07 to 0.29 indicating moderate level of genetic polymorphism. Hierarchical cluster analysis using SPSS method showed genetic variation amongst genotypes dividing them into three major clusters comprising 10, seven and one genotypes, respectively. The result of present study indicates that RAPD analysis has determined the genetic relationships and estimated the genetic diversity among the genotypes of P. ovata. Key words: Plantago ovata (Forsk.), random amplified polymorphic DNA (RAPD) markers, Nei and Li equation, genetic diversity

Genetic diversity analysis of the medicinal herb Plantago ovata (Forsk.)

Plantago ovata (Forsk.) (2n = 8) used as laxative, emollient and demulcent, has great commercial and medicinal importance. With India being the largest producer in the world there is still a lack of defined varieties of the species and no coordinated breeding efforts are being made. In the present study, we report the phylogenetic analysis of the crop for its utilization in future breeding programs for defining varieties of the crop. A total of 302 clear and reproducible bands were obtained with random amplified polymorphic DNA (RAPD) techniques involving 35 random primers in 18 selected lines, out of which 198 (65.5%) were polymorphic with an average 8.6 bands per primer. Amplified DNA fragments ranged from 300 to 3400 bp. Dissimilarity indices based on Nei and Li equation ranged from 0.07 to 0.29 indicating moderate level of genetic polymorphism. Hierarchical cluster analysis using SPSS method showed genetic variation amongst genotypes dividing them into three major clusters comprising 10, seven and one genotypes, respectively. The result of present study indicates that RAPD analysis has determined the genetic relationships and estimated the genetic diversity among the genotypes of P. ovata.Key words: Plantago ovata (Forsk.), random amplified polymorphic DNA (RAPD) markers, Nei and Li equation, genetic diversity

RAPD based DNA markers linked to anthracnose disease resistance in <i><span style="font-size:14.0pt;line-height:115%;font-family:"Times New Roman"; mso-fareast-font-family:"Times New Roman";color:black;mso-ansi-language:EN-IN; mso-fareast-language:EN-IN;mso-bidi-language:HI" lang="EN-IN">Sorghum bicolor</span></i><span style="font-size:14.0pt;line-height:115%;font-family:"Times New Roman"; mso-fareast-font-family:"Times New Roman";color:black;mso-ansi-language:EN-IN; mso-fareast-language:EN-IN;mso-bidi-language:HI" lang="EN-IN"> ( L.) Moench.</span>

206-211<span style="font-size:14.0pt;line-height: 115%;font-family:" times="" new="" roman";mso-fareast-font-family:"times="" roman";="" color:black;mso-ansi-language:en-in;mso-fareast-language:en-in;mso-bidi-language:="" hi"="" lang="EN-IN">Anthracnose caused by Colletotrichum graminicola is one of the major diseases of sorghum. The locus for disease resistance in sorghum [Sorghum biocolor (L.) Moench] accession G73 was found to segregate as a simple recessive trait in across to susceptible cultivar HC 136. In order to identify molecular markers linked to the locus for disease resistance, random amplificd polymorphic DNA (RAPD) analysis was coupled with bulk segregant analysis. DNA from the parental cultivars and the bulks were screened by PCR amplification with 114 RAPD primers. Three RAPD primers amplified a sequence that consegregated with the recessive resistance allele, while another three amplified a band linked to the susceptible allete. The six disease linked markers were screened with individual resistant and susceptible genotypes to observe degree of linkage of identified RAPD markers with the gene for resistance. Two primer sequences (OPI 16 and OPD 12) were found to be closely linked to the locus for disease resistance<span style="font-size:14.0pt; line-height:115%;font-family:Fd35851-Identity-H;mso-fareast-font-family:" times="" new="" roman";="" mso-bidi-font-family:fd35851-identity-h;color:black;mso-ansi-language:en-in;="" mso-fareast-language:en-in;mso-bidi-language:hi"="" lang="EN-IN">.</span

Correction to: A comparative genomic analysis of putative pathogenicity genes in the host-specific sibling species Colletotrichum graminicola and Colletotrichum sublineola

Following the publication of this article [1], the authors informed us of the following error