ECHO OF LODHRADI KASHAYA IN LIPID PROFILE OF TYPE 2 DIABETES MELLITUS PATIENTS

Objective: Lipid profile is matter to concern in fast changing lifestyle, and especially in diabetic mellitus (DM) patients. In arena of DM, obesity and high lipid contour is a causative problem and lead to generate the related problems too. Hence, tracking of lipid profile in DM patients is necessary as part of treatment approach. Hence, the present study was conducted to track the effect of Lodhradi Kashaya spray dried (LKSD) powder on lipid profile in Type 2 DM patients.Methods: Total 31 patients were taken following the guideline mention in Central Council for Research in Ayurveda and Siddha protocol for DM research. They were divided into two groups, Groups A and B and given LKSD (4 and 2 g TDS (three times a day), respectively, dissolved in water) for 3 months. They are investigated for their blood serum creatinine and lipid profile (cholesterol, low-density lipoprotein [LDL], high-density lipoprotein [HDL], very LDL [VLDL], and triglyceride). The patients response has also been noted regarding palatability acceptance and ease of administration.  Result: LKSD was found significantly effective in total cholesterol (p=0.001 and 0.073), triglyceride (p=0.002 and 0.003), HDL (pË‚0.001 and 0.026), LDL (p=0.563 and pË‚0.001), and VLDL (p=0.024 and 0.003). Patients had reported that formulation is easy to take in spite of its astringency in taste.Conclusion: Lodhradi Kashaya has capability to control and take care of disturbance in lipid profile in DM patients and thus enhance their living confidence.Â

Inhibition of Protein Aggregation: Supramolecular Assemblies of Arginine Hold the Key

BACKGROUND: Aggregation of unfolded proteins occurs mainly through the exposed hydrophobic surfaces. Any mechanism of inhibition of this aggregation should explain the prevention of these hydrophobic interactions. Though arginine is prevalently used as an aggregation suppressor, its mechanism of action is not clearly understood. We propose a mechanism based on the hydrophobic interactions of arginine. METHODOLOGY: We have analyzed arginine solution for its hydrotropic effect by pyrene solubility and the presence of hydrophobic environment by 1-anilino-8-naphthalene sulfonic acid fluorescence. Mass spectroscopic analyses show that arginine forms molecular clusters in the gas phase and the cluster composition is dependent on the solution conditions. Light scattering studies indicate that arginine exists as clusters in solution. In the presence of arginine, the reverse phase chromatographic elution profile of Alzheimer's amyloid beta 1-42 (Abeta(1-42)) peptide is modified. Changes in the hydrodynamic volume of Abeta(1-42) in the presence of arginine measured by size exclusion chromatography show that arginine binds to Abeta(1-42). Arginine increases the solubility of Abeta(1-42) peptide in aqueous medium. It decreases the aggregation of Abeta(1-42) as observed by atomic force microscopy. CONCLUSIONS: Based on our experimental results we propose that molecular clusters of arginine in aqueous solutions display a hydrophobic surface by the alignment of its three methylene groups. The hydrophobic surfaces present on the proteins interact with the hydrophobic surface presented by the arginine clusters. The masking of hydrophobic surface inhibits protein-protein aggregation. This mechanism is also responsible for the hydrotropic effect of arginine on various compounds. It is also explained why other amino acids fail to inhibit the protein aggregation

Management of Diabetes mellitus and its complications by Lodhra: A review

In Ayurveda, Lodhra is a used for many ailments either as single ingredient or part of formulation. It has been used for thousands of years to manage Kapha-Pitta based disease and specially to improve women's health in Ayurvedic science. Observations: Among these ailment, it was also indicated for Prameha (Diabetes) in many authoritative books of Ayurveda (First schedule of The Drugs and Cosmetics Act 1940). Contemporary science also proofs its antidiabetic property by recent researches. The clinical course and prognosis of health and life of diabetic patient are now largely determined by so called complications of diabetes. Other properties of Lodhra viz. healing property, antioxidant property, anti-microbial property, anti-ulcer property etc. may be utilized against diabetic complications. Aim and Objective: Author present here comprehensive review of Lodhra for its antidiabetic property and investigate its pharmacodynamics that may help to keep away diabetic complications and other problems of daily life.Â

Review on <i>Lodhradi Kashaya</i>: All-rounder remedy for Diabetes mellitus patients

100-106Diabetes has been elaborately discussed with their management approach in every medical system including Ayurveda. Peoples have mindset that only elevated blood sugar is diabetes while it is symptom of disturbed physiology within body and leading to continuous malfunctioning related with glucose utilization. Only blood glucose level is not the problem, but the complications arise due to the elevated blood glucose, malfunctioning of organs and its physiology is also area for concern. Synthetic chemical ingredients of allopathic medicine are serving well through its unique target treatment but its safety against efficacy is always questioned. Authors have opinion to manage diabetic syndrome using natural multi-ingredients approach that can trigger many target (blood sugar, receptor locking, blood transport cycle, utilization chain, insulin management, beta cell, nervous system and blood vessels) with its own properties (Hypoglycemic, antioxidant, hypolipidemic, cytoprotective, healing, etc.) along with synergistic effect of other ingredients. So, author picked a formulation, Lodhradi Kashaya from ancient wisdom that was decorated with word Madhumehajeet (Win over diabetes) in Basavarajiyam and also mentioned in Vaidya Chintamani for Prameha (Diabetes). In this review paper, authors analyse critically as well as reasonably the treatment approach as well as management tricks associated with Lodhradi Kashaya

Anti-hyperglycaemic study of <em>Eladi Churna</em> in streptozotocin (STZ) induced diabetic rats

34-40Diabetes mellitus is the seventh leading cause of death worldwide, with a frequent occurrence of Type II diabetes as compared to Type I. The aim of this present study was to evaluate the anti-hyperglycaemic effect of a classical herbomineral preparation Eladi Churna in STZ induced Type II diabetic rats. Thirty adult charles foster albino rats were allocated in to five groups with six animals in each group: Group I (Control Group), Group II (Diabetic control group without any medication), group III (Standard drug Glibenclamide at 1mg/kg of body weight), group IV (Eladi Churna at 300 mg/kg of body weight), group V (Eladi Churna at 600 mg/kg of body weight). Diabetes was induced by intraperitoneal injection of STZ at dose level of 35mg/kg. The whole study was conducted for 28 days. The animals were examined for blood glucose levels on 0, 7, 14, 21, 28 days respectively and other biochemical parameters such as serum cholestrol, HDL, LDL, Triglycerides, SGOT, SGPT were evaluated on day 0 and 28, respectively. The results showed that a gradual reduction in blood glucose level was assessed on administration of Eladi Churna at two different dose levels. But it was more significant at dose level of 600 mg/kg as p<0.05 with a mean±SD value of 107.34±2.67 on day 28 as compared to mean±SD value of 494.40±43.99 on day 3 (after induction of diabetes mellitus). And the results were almost analogous to potent antidiabetic drug glibenclamide with a mean±SD value of 95.44±7.44 on day 28. And the drug was also responsible for maintenance of alterations in other biochemical parameters, associated with diabetes mellitus, thus depicting its potent anti hyperglycaemic and hypolipidaemic actions

Clinical evaluation of <em>Talapotaka Churna</em> – A polyherbal <em>Ayurvedic</em> formulation in type 2 Diabetes mellitus

168-175To evaluate and compare the antidiabetic effect of Talapotaka Churna in Type II Diabetes mellitus patients were registered from S. S. Hospital, Banaras Hindu University, Varanasi. Patients were randomly divided in four groups for treatment of four different medicine groups: [Group A- Talapotaka Churna (4 g TID) with Takra (Buttermilk) before meal; Group B- Talapotaka Churna (4 g TID) with warm water before meal; Group C- Talapotaka Churna (4 g TID) with ongoing allopathic treatment with water before meal . Group D- Glimepride treated (1 mg BD) before meal]. The treatment was given for a period of two months. Fasting blood sugar, postprandial blood sugar, HbA1c, SGOT, SGPT, Sr. Creatinine and lipid profile level were estimated biochemically. The results were promising and reveal that Talapotaka Churna can be a safe, acceptable and effective alternative or adjuvant to the conventional oral hypoglycemic agents.Holistic principles of Ayurveda when used as supplementary for modern drugs in diabetes show their effects on all bodily symptoms and helps to minimize them in which modern drug fails. So, it is the need of time to support the modern medications with Ayurvedic therapy

Chemical Characterization of Lauha Bhasma by X-Ray Diffraction and Vibrating Sample Magnetometry

Herbomineral formulations are unique to Ayurveda. Lauha bhasma is an Ayurvedic metallic preparation used in iron deficiency anemia. Our emphasis in the present paper is on chemical characterization of Lauha bhasma at different steps by following X-ray Diffraction (XRD) study and Vibrating Sample Magnetometry (VSM) study. XRD study was conducted for the samples after Sthalipaka, 1st Puta, 5th Puta, 10th Puta, 15th Puta and 20th Puta and VSM study was conducted for samples after 5th 10th and 20th Puta. Fe3O4 and &gamma;-Fe2O3 may be accepted as chemical characterization of Lauha bhasma in broader way as it has been explored in this project by applying more sophisticated analytical technique like XRD and VSM