The Effect of High Glucocorticoid Administration and Food Restriction on Rodent Skeletal Muscle Mitochondrial Function and Protein Metabolism

Glucocorticoids levels are high in catabolic conditions but it is unclear how much of the catabolic effects are due to negative energy balance versus glucocorticoids and whether there are distinct effects on metabolism and functions of specific muscle proteins.We determined whether 14 days of high dose methylprednisolone (MPred, 4 mg/kg/d) Vs food restriction (FR, food intake matched to MPred) in rats had different effects on muscle mitochondrial function and protein fractional synthesis rates (FSR). Lower weight loss (15%) occurred in FR than in MPred (30%) rats, while a 15% increase occurred saline-treated Controls. The per cent muscle loss was significantly greater for MPred than FR. Mitochondrial protein FSR in MPred rats was lower in soleus (51 and 43%, respectively) and plantaris (25 and 55%) than in FR, while similar decline in protein FSR of the mixed, sarcoplasmic, and myosin heavy chain occurred. Mitochondrial enzymatic activity and ATP production were unchanged in soleus while in plantaris cytochrome c oxidase activity was lower in FR than Control, and ATP production rate with pyruvate + malate in MPred plantaris was 28% lower in MPred. Branched-chain amino acid catabolic enzyme activities were higher in both FR and MPred rats indicating enhanced amino acid oxidation capacity.MPred and FR had little impact on mitochondrial function but reduction in muscle protein synthesis occurred in MPred that could be explained on the basis of reduced food intake. A greater decline in proteolysis may explain lesser muscle loss in FR than in MPred rats

Is propensity to obesity associated with the diurnal pattern of core body temperature?

BACKGROUND: Obesity affects more than half a billion people worldwide, but the underlying causes remain unresolved. It has been proposed that propensity to obesity may be associated with differences between individuals in metabolic efficiency and in the energy used for homeothermy. It has also been suggested that obese-prone individuals differ in their responsiveness to circadian rhythms. We investigated both these hypotheses by measuring the core body temperature at regular and frequent intervals over a diurnal cycle, using indigestible temperature loggers in two breeds of canines known to differ in propensity to obesity, but prior to divergence in fatness. METHODS: Greyhounds (obesity-resistant) and Labradors (obesity-prone) were fed indigestible temperature loggers. Gastrointestinal temperature was recorded at 10-min intervals for the period of transit of the logger. Diet, body condition score, activity level and environment were similar for both groups. Energy digestibility was also measured. RESULTS: The mean core body temperature in obesity-resistant dogs (38.27 °C) was slightly higher (P<0.001) than in obesity-prone dogs (38.18 °C) and the former had a greater variation (P<0.001) in 24h circadian core temperature. There were no differences in diet digestibility. CONCLUSION: Canines differing in propensity to obesity, but prior to its onset, differed little in mean core temperature, supporting similar findings in already-obese and lean humans. Obese-prone dogs were less variable in daily core temperature fluctuations, suggestive of a degree of circadian decoupling.PI Hynd, VH Czerwinski and TJ McWhorte