Understory Bird Communities in Amazonian Rainforest Fragments: Species Turnover through 25 Years Post-Isolation in Recovering Landscapes

Inferences about species loss following habitat conversion are typically drawn from short-term surveys, which cannot reconstruct long-term temporal dynamics of extinction and colonization. A long-term view can be critical, however, to determine the stability of communities within fragments. Likewise, landscape dynamics must be considered, as second growth structure and overall forest cover contribute to processes in fragments. Here we examine bird communities in 11 Amazonian rainforest fragments of 1–100 ha, beginning before the fragments were isolated in the 1980s, and continuing through 2007. Using a method that accounts for imperfect detection, we estimated extinction and colonization based on standardized mist-net surveys within discreet time intervals (1–2 preisolation samples and 4–5 post-isolation samples). Between preisolation and 2007, all fragments lost species in an area-dependent fashion, with loss of as few as <10% of preisolation species from 100-ha fragments, but up to 70% in 1-ha fragments. Analysis of individual time intervals revealed that the 2007 result was not due to gradual species loss beginning at isolation; both extinction and colonization occurred in every time interval. In the last two samples, 2000 and 2007, extinction and colonization were approximately balanced. Further, 97 of 101 species netted before isolation were detected in at least one fragment in 2007. Although a small subset of species is extremely vulnerable to fragmentation, and predictably goes extinct in fragments, developing second growth in the matrix around fragments encourages recolonization in our landscapes. Species richness in these fragments now reflects local turnover, not long-term attrition of species. We expect that similar processes could be operating in other fragmented systems that show unexpectedly low extinction

Randomized controlled trials

This chapter covers the current gold standard for evaluating the effectiveness of therapeutic interventions, the randomized controlled trial (RCT). Key features of the RCT, regardless of sub-type, are randomization, allocation concealment, and blinding. These key features help reduce bias and the influence of confounding variables, making the randomized controlled trial eminently suitable to determine cause and effect relationships. Protocol design and registration prior to trial onset are important factors in determining the quality of the trial, and various trial design sub-types, including parallel, factorial, crossover, and cluster, are outlined and the strengths and weakness of each examined. Various checklists such as SPIRIT and CONSORT can be used to ensure proper reporting of both trial protocols and trial findings, to ensure clear, concise reporting. Finally, the shortcomings of RCTs and newer trial designs, such as comparative effectiveness research and pragmatic studies, designed to overcome some of these issues are examined, and ways to make clinical trial results more clinically applicable are discussed

Monkey and dung beetle activities influence soil seed bank structure

We investigated the influence of Neotropical dung beetles on soil seed bank structure after primary dispersal by the red howler monkey (Alouatta seniculus). We collected seeds from soil samples (up to 15 cm depth) in defecation versus control areas and showed that seed number and diversity increased with monkey frequentation. Seed numbers decreased with depth. Seed viability, ascertained from seed coat integrity, decreased with depth and was higher in sites rarely visited by monkeys compared to control areas or sites frequently visited by monkeys. In field experiments, we incorporated plastic beads (1.3 – 5.8 mm) to fresh dung and monitored bead fate: the proportion of beads found in the soil top 10 cm increased with bead size and this effect was more pronounced in sites more frequently visited by monkeys. The same conclusions were drawn by comparing the beads found in the topsoil and the beads found deeper. We explored bead processing behaviour in several tunneller and roller species by performing experiments involving one species at a time. We showed that selectivity was highly variable: bead exclusion from dung reserves was higher in small than in large beetle species, higher for large than for small beads. Differences in selectivity between medium an