Drosophila-Chinese hamster hybrid cells from cell lines adapted to grow in the same environmental conditions

SUMMARYEvidence is given concerning the possible production of Drosophila melanogaster-Chinese hamster hybrid cells, after PEG mediated fusion experiments between cell lines adapted to grow in the same environmental conditions: 31o C and a modified mammalian medium. However, autoradiograpy failed to detect viable complementation in Drosophila-Chinese hamster TK− heterokaryons, and this is consistent with the failure to obtain proliferating hybrids in a semiselective HAT medium

Immunological Prophile of FA. A Multicentric retrospective Analysis of 61 Patients

Transplantation and immunomodulatio

Chronic myelomonocytic leukemia in childhood: A retrospective analysis of 110 cases

Chronic myelomonocytic leukemia (CMML) is a rare hematopoietic malignancy of childhood. To define the clinical and hematologic characteristics of the disease, we performed a retrospective analysis of 110 children given the diagnosis CMML irrespective of karyotype. Median age at diagnosis was 1.8 years. Neurofibromatosis type 1 was known in 14% and other clinical abnormalities in 7% of the children. At presentation, the medium white blood count was 35 x 109/L, with a median monocyte count of 7 x 109/L. Karyotypic abnormalities in bone marrow cells were noted in 36% of the patients, whereas 26% of the children had monosomy 7. Children with monosomy 7 did not differ from those with normal karyotype with respect to their clinical presentation. However, they did display some characteristic hematologic features. Of 110 children, 38 received an allogeneic bone marrow transplant (BMT). The probability of survival at 10 years was 0.39 (standard error [SE] = 0.10) for the BMT group and 0.06 (SE = 0.4) for the 72 patients of the non-BMT group. Platelet count, age, and hemoglobin F at diagnosis were the main predicting factors for the length of survival in the non-BMT group. There is a strong need for a broad agreement on nomenclature in children with myelodysplastic syndromes (MDS). We propose here to use the French-American- British classification for MDS in childhood

Chronic myelomonocytic leukemia in childhood. A retrospective analysis of 110 cases.

none16Chronic myelomonocytic leukemia (CMML) is a rare hematopoietic malignancy of childhood. To define the clinical and hematologic characteristics of the disease, we performed a retrospective analysis of 110 children given the diagnosis CMML irrespective of karyotype. Median age at diagnosis was 1.8 years. Neurofibromatosis type 1 was known in 14% and other clinical abnormalities in 7% of the children. At presentation, the medium white blood count was 35 x 10(9)/L, with a median monocyte count of 7 x 10(9)/L. Karyotypic abnormalities in bone marrow cells were noted in 36% of the patients, whereas 26% of the children had monosomy 7. Children with monosomy 7 did not differ from those with normal karyotype with respect to their clinical presentation. However, they did display some characteristic hematologic features. Of 110 children, 38 received an allogeneic bone marrow transplant (BMT). The probability of survival at 10 years was 0.39 (standard error [SE] = 0.10) for the BMT group and 0.06 (SE = 0.4) for the 72 patients of the non-BMT group. Platelet count, age, and hemoglobin F at diagnosis were the main predicting factors for the length of survival in the non-BMT group. There is a strong need for a broad agreement on nomenclature in children with myelodysplastic syndromes (MDS). We propose here to use the French-American-British classification for MDS in childhoodnoneCM. NIEMEYER; M. ARICÒ; G. BASSO; A. BIONDI; A. CANTÙ-RAJNOLDI; U. CREUTZIG; O. HAAS; J. HARBOTT; H. HASLE; G. KERNDRUP; F. LOCATELLI; G. MANN; B. STOLLMAN-GIBBELS; ETH. VAN'T VEER-KORTHOF; E. VAN WERING; M. ZIMMERMANNNiemeyer, C. M.; M., Aricò; Basso, Giuseppe; A., Biondi; A., CANTÙ RAJNOLDI; U., Creutzig; O., Haas; J., Harbott; H., Hasle; G., Kerndrup; F., Locatelli; G., Mann; B., STOLLMAN GIBBELS; VAN'T VEER KORTHOF, E. T. H.; E., VAN WERING; M., Zimmerman

Haematopoietic stem cell transplantation for Shwachman-Diamond disease: a retrospective survey from EBMT registry

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