Simultaneous Quantification Of Phenolic Compounds In Buriti Fruit (mauritia Flexuosa L.f.) By Ultra-high Performance Liquid Chromatography Coupled To Tandem Mass Spectrometry

A novel analytical approach based on UHPLC-ESI(-)-MS/MS has been performed and evaluated for the identification and quantification of phenolic compounds (phenolic acids and flavonoids/catechins) in buriti (Mauritia flexuosa L.f.) fruit pulp. Due to the selectivity provided by MMR monitoring, UHPLC separation required only 10min per analysis. Linearity (0.994<r2<0.999), limit of detection (8.39-109.80pgmL-1) and limit of quantification (25.42-332.90pgmL-1), inter- and intraday accuracies (>80%) and precisions (CV<20%), and recovery rate (<80%) show that the developed method is reliable to analyze phenolic compounds in fruit extracts. Among the thirteen phenolic compounds analyzed by proposed method, buriti fruit pulp extract showed 6 phenolic acids such as p-coumaric acid, ferulic acid, caffeic acid, protocatechuic acid, chlorogenic acid and quinic acid and 7 flavonoids such as (+)-catechin, (-)-epicatechin, apigenin, luteolin, myricetin, kaempferol and quercetin. As a conclusion, the method was successfully developed and applied representing a contribution to the chemical characterization and quantification of phenolic compounds from buriti fruits which have acknowledged antioxidant and antimicrobial activities.66396400Alessandri, S., Ieri, F., Romani, A., Minor polar compounds in extra virgin olive oil: Correlation between HPLC-DAD-MS and the Folin-Ciocalteu spectrophotometric method (2014) Journal of Agricultural and Food Chemistry, 62, pp. 826-835Delgado, C., Couturier, G., Mejia, K., Mauritia flexuosa (Arecaceae: Calamoideae), an Amazonian palm with cultivation purposes in Peru (2007) Fruits, 62, pp. 157-169Engels, C., Gräter, D., Esquivel, P., Jiménez, V.M., Gänzle, M.G., Schieber, A., Characterization of phenolic compounds in jocote (Spondias purpurea L.) peels by ultra high-performance liquid chromatography/electrospray ionization mass spectrometry (2012) Food Research International, 46, pp. 557-562Godoy-Caballero, M.P., Acedo-Valenzuela, M.I., Galeano-Diaz, T., New reversed phase dispersive liquid-liquid microextraction method for the determination of phenolic compounds in virgin olive oil by rapid resolution liquid chromatography with ultraviolet-visible and mass spectrometry detection (2013) Journal of Chromatography A, 1313, pp. 291-301Goméz-Alonso, S., García-Romero, E., Hermosín-Gutierrez, I., HPLC analysis o diverse grape and wine phenolics using direct injection and multidetection by DAD and fluorescence (2007) Journal of Food Composition and Analysis, 20, pp. 618-622Grundya, H.H., Read, W.A., Macarthur, R., Dickinson, M., Charlton, A.J., Dusek, M., Selected reaction monitoring method to determine the species origin of blood-based binding agents in meats: A collaborative study (2013) Food Chemistry, 141, pp. 3351-3536Koolen, H.H.F., da Silva, F.M.A., Gozzo, F.C., de Souza, A.Q.L., de Souza, A.D.L., Antioxidant, antimicrobial activities and characterization of phenolic compounds from buriti (Mauritia flexuosa L.f.) by UPLC-ESI-MS/MS (2013) Food Research International, 51, pp. 467-473Koolen, H.H.F., Soares, E.R., da Silva, F.M.A., de Oliveira, A.A., de Souza, A.Q.L., Medeiros, L.S., Mauritic acid: A new dammarane triterpene from the roots of Mauritia flexuosa L.f. (Arecaceae) (2013) Natural Product Research, 27, pp. 2118-2125Koolen, H.H.F., Soares, E.R., da Silva, F.M.A., de Souza, A.Q.L., de Medeiros, L.S., Rodrigues-Filho, E., de Almeida, R.A., de Souza, A.D.L., An antimicrobial diketopiperazine alkaloid and co-metabolites from an endophytic strain of Gliocladium isolated from Strychnos cf. toxifera (2012) Natural Product Research, 26, pp. 2013-2019Koolen, H.H.F., Soares, E.R., da Silva, F.M.A., de Souza, A.Q.L., Rodrigues-Filho, E., de Souza, A.D.L., Triterpenes and flavonoids from Mauritia flexuosa (2012) Revista Brasileira de Farmacognosia, 22, pp. 189-192Liu, P., Kallio, H., Yang, B., Flavonol glycosides and other phenolic compounds in buds and leaves of different varieties of black currant (Ribes nigrum L.) and changes during growing season (2014) Food Chemistry, 160, pp. 180-189Malta, L.G., Tessaro, E.P., Eberlin, M., Pastore, G.M., Liu, R.H., Assessment of antioxidant and antiproliferative activities and the identification of phenolic compounds of exotic Brazilian fruits (2013) Food Research International, 53, pp. 417-425Memon, A.A., Memon, N., Bhanger, M.I., Luthria, D.L., Assay of phenolic compounds from four species of Ber (Ziziphus mauritiana L.) fruits: Comparison of three base hydrolysis procedure for quantification of total phenolic acids (2013) Food Chemistry, 139, pp. 496-502Morales-Soto, A., García-Salas, P., Rodríguez-Pérez, C., Jiménez-Sánchez, C., Cádiz-Gurrea, M.L., Segura-Carretero, A., Antioxidant capacity of 44 cultivars of fruits and vegetables grown in Andalusia (Spain) (2014) Food Research International, 58, pp. 35-46Ramirez-Ambrosi, M., Abad-Garcia, B., Viloria-Bernal, M., Garmon-Lobato, S., Berrueta, L.A., Gallo, B., A new ultrahigh performance liquid chromatography with diode array detection coupled to electrospray ionization and quadrupole time-of-flight mass spectrometry analytical strategy for fast analysis and improved characterization of phenolic compounds in apple products (2013) Journal of Chromatography A, 1316, pp. 78-91Xi, W., Zhang, Y., Sun, Y., Shen, Y., Ye, X., Zhou, Z., Phenolic composition of Chinese wild mandarin (Citrus reticulata Blanco.) pulps and their antioxidant properties (2014) Industrial Crops and Products, 52, pp. 466-47

Comprehensive Characterization Of Lipids From Amazonian Vegetable Oils By Mass Spectrometry Techniques

An integrative approach in mass spectrometry (MS) comprising gas chromatography coupled to MS (GC-MS), ultra-efficiency liquid chromatography coupled to MS (UPLC-MS) and easy ambient sonic-spray ionization MS (EASI-MS) is proposed for the comprehensive characterization of Amazonian oils. Coconut, andiroba and castor seed oils, which are vastly sold in markets of the Amazonian region of Brazil, were selected as a representative test set. These oils were found to contain several lipids such as triacylglycerides (TAGs), fatty acids (FAs), phytosterols and limonoids. In the analyzed samples 30 different TAGs, 11 FAs, 6 phytosterols and 7 limonoids were identified. The antioxidant capacity (AOC) of the oils, as measured by their oxygen radical absorbance capacity (ORAC), was also used to evaluate their potential biological properties as well as their possible consumption as food. Edible virgin coconut oil was the most active (0.720. ±. 0.001 Trolox eq./mmol), whereas considerable lower activity was observed for andiroba and castor seed oils. The antimicrobial activities of the oils were also recorded against a panel of pathogenic bacteria and fungi in which andiroba oil was the only one that was active, solely against Enterococcus aeruginosa. © 2014 Elsevier Ltd.64472481Akande, T.O., Odunsi, A.A., Adedeji, O.S., Toxicity and nutritive assessment of castor oil (Ricinus communis) oil and processed cake in rate diet (2011) Asian Journal of Animal Sciences, 5, pp. 330-339Ambrozin, A.R.P., Leite, A.C., Bueno, F.C., Vieira, P.C., Fernandes, J.B., Bueno, O.C., Limonoids from andiroba oil and Cedrela fissilis and their insecticidal activity (2006) Journal of the Brazilian Chemical Society, 17, pp. 542-547Barbosa, B.S., Koolen, H.H.F., Barreto, A.C., da Silva, J.D., Figlioulo, R., Nunomura, S.M., The use of tucumã of Amazonas kernel oil in the biodiesel production (2009) Acta Amazonica, 39, pp. 371-376Breksa, A.P., Manners, G.D., Evaluation of the antioxidant capacity of limonin, nomilin, and limonin glucoside (2006) Journal of Agricultural and Food Chemistry, 54, pp. 3827-3831Cabral, E.C., da Cruz, G.F., Simas, R.C., Sanvido, G.B., Gonçalves, L.V., Leal, R.V.P., Typification and quality control of the andiroba (Carapa guianensis) oil via mass spectrometry fingerprinting (2013) Analytical Methods, 5, pp. 1385-1391César, A.S., Batalha, M.O., Biodiesel production from castor oil in Brazil: A difficult reality (2010) Energy Policy, 38, pp. 4031-4039Chakraborty, M., Mitra, A., The antioxidant and antimicrobial activity properties of the methanolic extract from Cocos nucifera mesocarp (2008) Food Chemistry, 107, pp. 994-999Chen, J., Wu, X., Zhang, Y., Relationship between antioxidants and acrylamide formation: A review (2013) Food Research International, 51, pp. 611-620Cherif, A.O., Leveque, N., Messaoudac, M.B., Kallel, H., Moussa, F., An electrospray ionisation-mass spectrometry screening of triacylglycerols in developing cultivated and wild peanut kernels (Arachis hypogaea L.) (2013) Food Chemistry, 138, pp. 1095-1100da Silva, V.P., Oliveira, R.R., Figueiredo, M.R., Isolation of limonoids from seeds of Carapa guianensis Aublet (Meliaceae) by high-speed countercurrent chromatography (2009) Phytochemical Analysis, 20, pp. 77-81Dávalos, A., Gómez-Cordovés, C., Bartolomé, B., Extending applicability of the oxygen radical absorbance capacity (ORAC-fluorescein) assay (2004) Journal of Agricultural and Food Chemistry, 52, pp. 48-54Dudonné, S., Vitrac, X., Coutière, P., Woillez, M., Mérillon, J.M., Comparative study of antioxidant properties and total phenolic content of 30 plant extracts of industrial interest using DPPH, ABTS, FRAP, SOD, and ORAC assays (2009) Journal of Agricultural and Food Chemistry, 57, pp. 1768-1774Funasaki, M., Oliveira, R.S., Zanotto, S.P., Carioca, C.R.F., Simas, R.C., Eberlin, M.N., Brazil nut oil: Quality control via triacylglycerol profiles provided by easy ambient sonic-spray ionization mass spectrometry (2012) Journal of Agricultural and Food Chemistry, 60, pp. 11263-11267Haddad, R., Milagres, H.M.S., Catharino, R.R., Eberlin, M.N., Easy ambient sonic-spray ionization mass spectrometry combined with thin-layer chromatography (2008) Analytical Chemistry, 80, pp. 2744-2750Hamburger, M., Riese, U., Graf, H., Melzig, M.F., Ciesielski, S., Baumann, D., Constituents in evening primrose oil with radical scavenging, cyclooxygenase, and neutrophil elastase inhibitory activities (2002) Journal of Agricultural and Food Chemistry, 50, pp. 5533-5538Kesena, S., Kelebek, H., Senc, K., Ulas, M., Selli, S., GC-MS-olfactometric characterization of the key aroma compounds in Turkish olive oils by application of the aroma extract dilution analysis (2013) Food Research International, 54, pp. 1987-1994Koolen, H.H.F., da Silva, F.M.A., Gozzo, F.C., de Souza, A.Q.L., de Souza, A.D.L., Antioxidant, antimicrobial activities and characterization of phenolic compounds from buriti (Mauritia flexuosa L.f.) by UPLC-ESI-MS/MS (2013) Food Research International, 51, pp. 467-473Koolen, H.H.F., Soares, E.R., da Silva, F.M.A., de Almeida, R.A., de Souza, A.D.L., de Medeiros, L.S., An antimicrobial alkaloid and other metabolites produced by Penicillium sp. An endophytic fungus isolated from Mauritia flexuosa L.f. (2012) Quimica Nova, 35, pp. 771-774Koolen, H.H.F., Soares, E.R., da Silva, F.M.A., de Souza, A.Q.L., de Medeiros, L.S., Rodrigues-Filho, E., An antimicrobial diketopiperazine alkaloid and co-metabolites from an endophytic strain of Gliocladium isolated from Strychnos cf. toxifera (2012) Natural Product Research, 26, pp. 2013-2019Lay, J.O., Linayage, R., Durham, B., Brooks, J., Rapid characterization of edible oils by direct matrix-assisted laser desorption/ionization time-of-flight mass spectrometry analysis using triacylglycerols (2006) Rapid Communications in Mass Spectrometry, 20, pp. 952-958Lemire, M., Fillion, M., Barbosa-Júnior, F.B., Guimarães, J.R.D., Mergler, D., Elevated levels of selenium in the typical diet of Amazonian riverside populations (2010) Science of the Total Environment, 408, pp. 4076-4084Liu, C., Li, J., Lei, W., Zhou, Y., Development of biobased unsaturated polyester resin containing highly functionalized castor oil (2014) Industrial Crops and Products, 52, pp. 329-337Marina, A.M., Man, Y.B.C., Ismail, A., Virgin coconut oil: Emerging functional food oil (2009) Trends in Food Science and Technology, 20, pp. 481-487Marina, A.M., Man, Y.B.C., Nazimah, S.A.H., Amin, I., Antioxidant capacity and phenolic acids of virgin coconut oil (2009) International Journal of Food Sciences and Nutrition, 60, pp. 114-123Mendonça, F.A.C., Silva, K.F.S., Santos, K.K., Ribeiro-Júnior, K.A.L., Activities of some Brazilian plants against larvae of the mosquito Aedes aegypti (2005) Fitoterapia, 76, pp. 629-636. , Sant'AnaMiranda-Júnior, R.N.C., Dolabela, M.F., da Silva, M.N., Póvoa, M.M., Maia, J.G.S., Antiplasmodial activity of the andiroba (Carapa guianensis Aubl., Meliaceae) oil and its limonoid-rich fraction (2012) Journal of Ethnopharmacology, 142, pp. 679-683Nazif, N.M., Phytoconstituents of Zizyphus spina-christi L. fruits and their antimicrobial activity (2002) Food Chemistry, 76, pp. 77-81Oliveira, J.F.G., Lucena, I.L., Saboya, R.M.A., Rodrigues, M.L., Torres, A.E.B., Fernandes, F.A.N., Biodiesel production from waste coconut oil by esterification with ethanol: The effect of water removal by adsorption (2010) Renewable Energy, 35, pp. 2581-2584Ollis, W.D., Ward, A.D., de Oliveira, H.M., Zelnik, R., Andirobin (1970) Tetrahedron, 26, pp. 1637-1645Oloyede, G.K., Antioxidant activities of methyl ricinoleate and ricinoleic acid dominated Ricinus communis seeds extract using lipid peroxidation and free radical scavenging methods (2012) Journal of Medicinal Plant, 6, pp. 511-520Pérez-Jímenez, J., Arranz, S., Tabernero, M., Díaz-Rúbio, M.E., Serrano, J., Goñi, I., Updated methodology to determine antioxidant capacity in plant foods, oils and beverages: Extraction, measurement and expression of results (2008) Food Research International, 41, pp. 274-285Pesce, C., (1985) Oil palms and other oilseeds of the Amazon, pp. 61-151. , Johnson, D. V., Algonac, MIPoulose, S.M., Harris, E.D., Patil, B.S., Citrus limonoids induce apoptosis in human neuroblastoma cells and have radical scavenging activity (2005) Journal of Nutrition, 135, pp. 870-877Prior, R.L., Wu, X., Schaich, K., Standardized methods for the determination of antioxidant capacity and phenolics in foods and dietary supplements (2005) Journal of Agricultural and Food Chemistry, 53, pp. 4290-4302Qi, S.H., Wu, D.G., Zhang, S., Luo, X.D., Constituents of Carapa guianensis Aubl. (Meliaceae) (2004) Pharmazie, 59, pp. 488-490Roginsky, V., Lissi, E.A., Review of methods to determine chain-breaking antioxidant activity in food (2005) Food Chemistry, 92, pp. 235-254Roma, G.C., Vendramini, M.C.R., Mathias-Camargo, M.I., Nunes, P.H., de Faria, A.U., Bechara, G.H., Action of andiroba oil and permethrin on the central nervous and reproductive systems of Rhipicephalus sanguineu (Latreille, 1806) (Acari: Ixodidae) ticks females. A confocal study (2013) Research in Veterinary Science, 95, pp. 529-536Rostad, C.E., Pereira, W.E., Kovats and Lee retention indices determined by gas chromatography/mass spectrometry for organic compounds of environmental interest (1986) Journal of High Resolution Chromatography & Chromatography Communications, 9, pp. 328-334Salvador, M.J., Ferreira, E.O., Pral, E.M.F., Alfieri, S.C., Albuquerque, S., Ito, I.Y., Bioactivity of crude extracts and some constituents of Blutaparon portulacoides (Amaranthaceae) (2002) Phytomedicine, 9, pp. 566-571Santos, R.C.V., Alves, C.F.S., Schneider, T., Lopes, L.Q.S., Aurich, C., Giongo, J.L., Brandelli, A., Vaucher, R.A., Antimicrobial activity of Amazonian oils against Paenibacillus species (2012) Journal of Invertebrate Pathology, 109, pp. 265-268Saraiva, S.A., Cabral, E.C., Eberlin, M.N., Catharino, R.R., Amazonian vegetable oils and fats: Fast typification and quality control via triacylglycerol (TAG) profiles from dry matrix-assisted laser desorption/ionization (MALDI-TOF) mass spectrometry fingerprinting (2009) Journal of Agricultural and Food Chemistry, 57, pp. 4030-4034Shoba, F.G., Thomas, M., Study of antidiarrhoeal activity of four medicinal plants in castor oil-induced diarrhea (2001) Journal of Ethnophacology, 76, pp. 73-76Silva, S.G., Nunomura, R.S., Nunomura, S.M., Limonóides isolados dos frutos de Carapa guianensis Aublet (Meliaceae) (2012) Quimica Nova, 35, pp. 1936-1939Silva, V.P., Oliveira, R.R., Figueiredo, M.R., Isolation of limonoids from seeds of Carapa guianenses Aublet. (Meliaceae) by high-speed counter current chromatography (2009) Phytochemical Analysis, 20, pp. 77-81Solis, M.I.V., Patel, A., Orsat, V., Singhb, J., Lefsruda, M., Fatty acid profiling of the seed oils of some varieties of field peas (Pisum sativum) by RP-LC/ESI-MS/MS: Towards the development of an oilseed pea (2013) Food Chemistry, 139, pp. 986-993Souza, J.N.S., Silva, E.M., Loiir, A., Rees, J.F., Rogez, H., Larondelle, Y., Antioxidant capacity of four polyphenol-rich Amazonian plant extracts: A correlation study using chemical and biological in vitro assays (2008) Food Chemistry, 106, pp. 331-339Sun, C., Chen, K., Chen, Y., Chen, Q., Contents and antioxidant capacity of limonin and nomilin in different tissues of citrus fruit of four cultivars during fruit growth and maturation (2005) Food Chemistry, 93, pp. 599-605Tan, C.P., Man, Y.B.C., Differential scanning calorimetric analysis of edible oils: Comparison of thermal properties and chemical composition (2000) Journal of the American Oil Chemist's Society, 77, pp. 143-155Tappin, M.R.R., Nakamura, M.J., Siani, A.C., Lucchetti, L., Development of an HPLC method for the determination of tetranortriterpenoids in Carapa guianensis seed oil by experimental design (2008) Journal of Pharmaceutical and Biomedical Analysis, 48, pp. 1090-1095Tian, Q., Schwartz, S.J., Mass spectrometry and tandem mass spectrometry of citrus limonoids (2003) Analytical Chemistry, 75, pp. 5451-5460Willerding, A.L., Carvalho-Neto, F.G.M.R., da Gama, A.M., Carioca, C.R.F., de Oliveira, L.A., Hydrolytic activity of bacterial lipases in Amazonian vegetable oils (2012) Quimica Nova, 35, pp. 1782-1785Yamamoto, T., Ogura, M., Amano, A., Adachi, K., Hagiwara, T., Kanisawa, T., Synthesis and odor of optically active 2-n-hexyl- and 2-n-heptylcyclopentanone and the corresponding δ-lactones (2002) Tetrahedron Letters, 43, pp. 9081-9084Zhao, C.X., Liang, Y.Z., Fang, H.Z., Li, X.N., Temperature-programmed retention indices for gas chromatography-mass spectroscopy analysis of plant essential oils (2006) Journal of Chromatography A, 1096, pp. 76-8

Talaroxanthone, A Novel Xanthone Dimer From The Endophytic Fungus Talaromyces Sp. Associated With Duguetia Stelechantha (diels) R. E. Fries

DgCr22.1b, an endophytic isolate of Talaromyces sp., was collected in the Amazonian Rainforest from the medicinal plant Duguetia stelechantha. From the fractionation of the methanolic mycelial extract, a new xanthone dimer talaroxanthone was isolated. The structure of this new compound was elucidated based on spectroscopic analyses including 2D nuclear magnetic resonance (NMR) experiments. © 2013 Sociedade Brasileira de Química.245880883Liu, F., Cai, X.L., Yang, H., Xia, X.K., Guo, Z.Y., Yuan, J., Li, M.F., Lin, Y.C., (2010) Planta Med., 76, p. 185Dong, Y., Yang, J., Zhang, H., Lin, J., Ren, X., Liu, M., Lu, X., He, J., (2006) J. Nat. Prod., 69, p. 128Dethoup, T., Manoch, L., Kijjoa, A., Pinto, M., Gales, L., Damas, A.M., Silva, A.M.S., Herz, W., (2007) J. Nat. Prod., 70, p. 1200Li, L.Q., Yang, Y.G., Zeng, Y., Zou, C., Zhao, P.J., (2010) Molecules, 15, p. 3993Masters, K.S., Brase, S., (2012) Chem. Rev., 112, p. 3712Cao, S., McMillin, D.W., Tamayo, G., Delmore, J., Mitsiades, C.S., Clardy, J., (2012) J. Nat. Prod., 75, p. 793Krohn, K., Kouam, S.F., Kuigoua, G., Hussain, H., Brandt, S.C., Florke, U., Kurtán, T., Schulz, B., (2009) Chem. Eur. J., 15, p. 12121Isaka, M., Jaturapat, A., Rukseree, K., Danwisetkanjana, K., Tanticharoen, M., Thebtaranonth, Y., (2001) J. Nat. Prod., 64, p. 1015Pornpakakul, S., Liangsakul, J., Ngamrojanavanich, N., Roengsumran, S., Sihanonth, P., Piapukiew, J., Sangvichien, E., Petsom, A., (2006) Arch. Pharmacal Res., 29, p. 140Zhang, W., Krohn, K., Ullah, Z., Flçrke, U., Pescitelli, G., Di Bari, L., Antus, S., Schulz, B., (2008) Chem. Eur. J., 14, p. 4913Koolen, H.H.F., Soares, E.R., Silva, F.M.A., Almeida, R.A., Souza, A.D.L., Medeiros, L.S., Rodrigues Filho, E., Souza, A.Q.L., (2012) Quim. Nova, 35, p. 771Yang, D.M., Takeda, N., Iitaka, Y., Sankawa, U., Shibata, S., (1973) Tetrahedron, 29, p. 519Isaka, M., Palasarn, S., Kocharin, K., Saenboonrueng, S., (2005) J. Nat. Prod., 68, p. 945Franck, B., (1980) The Biosynthesis of the Ergochromes - A Study in Secondary Metabolism, 1. , 1st ed. Academic Press: London, UKAndersen, R., Buchi, G., Kobbe, B., Demain, A.L., (1977) J. Org. Chem., 42, p. 352Kurobane, I., Vining, L.C., McInnes, A.G., (1979) J. Antibiot., 32, p. 1256Steyn, P.S., (1970) Tetrahedron, 26, p. 51Souza, A.D.L., Rodrigues Filho, E., Souza, A.Q.L., Pereira, J.O., Calgarotto, K., Maso, V., Marangoni, S., Silva, S.L., (2008) Toxicon, 51, p. 240Souza, A.Q.L., Souza, A.D.L., Astolfi Filho, S., Pinheiro, M.L.B., Sarquis, M.I.M., Pereira, J.O., (2004) Acta Amazon., 34, p. 18

Chemical Composition And Antimicrobial Activity Of The Essential Oils Of Onychopetalum Amazonicum R.e.fr.

The essential oils from leaves, twigs and trunk bark of Onychopetalum amazonicum R.E. Fr. (Annonaceae), obtained by hydrodistillation, were analysed by GC and GC–MS, and also were evaluated for in vitro antimicrobial activity. Forty-one compounds, which correspond to 75.0–92.2% of the oil components, were identified. Major compounds were sesquiterpenes, including (E)-caryophyllene, caryophyllene oxide, spathulenol, α-gurjunene, allo-aromadendrene and α-epi-cadinol. The oils were evaluated for antimicrobial activities against four bacteria strains and five pathogenic fungi. The oil of the trunk bark exhibited good activity against Staphylococcus epidermidis ATCC 12228, Escherichia coli ATCC 10538 and Kocuria rhizophila ATCC 9341, with a minimal inhibitory concentration of 62.5 μg/mL. The essential oil composition and the antimicrobial evaluation are reported for the first time for the genus Onychopetalum. © 2016 Informa UK Limited, trading as Taylor & Francis Group.30202356235