Successfull Blossom Thinning and Crop Load Regulation for Organic Apple Growing with Potassium-bi-carbonate (Armicarb(R)): Results of Field Experiments over 3 Years and with 11 Cultivars

With field trials over 3 years in a commercial organic orchard in Switzerland we have tested the efficacy of Armicarb® (potassium-bi-carbonate) for flower thinning in organic apple production. Over time, Armicarb was tested on 11 cultivars, at different application periods, in different concentrations, and always in comparison to other agents that are already allowed for thinning in organic fruit production in the European Union as e.g. lime sulphur, molasses, mechanical rope-thinner or combinations of methods. Armicarb proved to be an efficient and reliable thinning agent with an efficacy similar to the now recommended methods with rope device, molasses or lime sulphur but has the advantage to be an environmentally very friendly product. On the other hand, the risk for fruit russeting is comparably elevated especially with cultivars ‘Elstar’, ‘Golden Del.’ ’and ‘Gala’. Finally, we have elaborated cultivar-specific recommendations for the use of Armicarb for thinning purposes, which were the basis for the Swiss Federal approval to use Armicarb for thinning in conventional and organic apple production in 2011/2012

Thermalization of magnons in yttrium-iron garnet: nonequilibrium functional renormalization group approach

Using a nonequilibrium functional renormalization group (FRG) approach we calculate the time evolution of the momentum distribution of a magnon gas in contact with a thermal phonon bath. As a cutoff for the FRG procedure we use a hybridization parameter {\Lambda} giving rise to an artificial damping of the phonons. Within our truncation of the FRG flow equations the time evolution of the magnon distribution is obtained from a rate equation involving cutoff-dependent nonequilibrium self-energies, which in turn satisfy FRG flow equations depending on cutoff-dependent transition rates. Our approach goes beyond the Born collision approximation and takes the feedback of the magnons on the phonons into account. We use our method to calculate the thermalization of a quasi two-dimensional magnon gas in the magnetic insulator yttrium-iron garnet after a highly excited initial state has been generated by an external microwave field. We obtain good agreement with recent experiments.Comment: 16 pages, 6 figures, final versio

Constraining the Hadronic Contributions to the Muon Anomalous Magnetic Moment

The mini-proceedings of the Workshop on "Constraining the hadronic contributions to the muon anomalous magnetic moment" which included the "13th meeting of the Radio MonteCarLow WG" and the "Satellite meeting R-Measurements at BES-III" held in Trento from April 10th to 12th, 2013, are presented. This collaboration meeting aims to bring together the experimental e+e- collider communities from BaBar, Belle, BESIII, CMD2, KLOE, and SND, with theorists working in the fields of meson transitions form factors, hadronic contributions to (g-2)_\mu and effective fine structure constant, and development of Monte Carlo generator and Radiative Corrections for precision e+e- and tau physics.Comment: 45 pages, 17 contributions. Editors: P. Masjuan and G. Venanzon

Mediat. Inflamm.

There is increasing evidence that proteasomes have a biological role in the extracellular alveolar space, but inflammation could change their composition. We tested whether immunoproteasome protein-containing subpopulations are present in the alveolar space of patients with lung inflammation evoking the acute respiratory distress syndrome (ARDS). Bronchoalveolar lavage (BAL) supernatants and cell pellet lysate from ARDS patients (n = 28) and healthy subjects (n = 10) were analyzed for the presence of immunoproteasome proteins (LMP2 and LMP7) and proteasome subtypes by western blot, chromatographic purification, and 2D-dimensional gelelectrophoresis. In all ARDS patients but not in healthy subjects LMP7 and LMP2 were observed in BAL supernatants. Proteasomes purified from pooled ARDS BAL supernatant showed an altered enzyme activity ratio. Chromatography revealed a distinct pattern with 7 proteasome subtype peaks in BAL supernatant of ARDS patients that differed from healthy subjects. Total proteasome concentration in BAL supernatant was increased in ARDS (971 ng/mL perpendicular to 1116 versus 59 perpendicular to 25; P < 0.001), and all fluorogenic substrates were hydrolyzed, albeit to a lesser extent, with inhibition by epoxomicin (P = 0.0001). Thus, we identified for the first time immunoproteasome proteins and a distinct proteasomal subtype pattern in the alveolar space of ARDS patients, presumably in response to inflammation

The nature of iron-oxygen vacancy defect centers in PbTiO3

The iron(III) center in ferroelectric PbTiO3 together with an oxygen vacancy forms a charged defect associate, oriented along the crystallographic c-axis. Its microscopic structure has been analyzed in detail comparing results from a semi-empirical Newman superposition model analysis based on finestructure data and from calculations using density functional theory. Both methods give evidence for a substitution of Fe3+ for Ti4+ as an acceptor center. The position of the iron ion in the ferroelectric phase is found to be similar to the B-site in the paraelectric phase. Partial charge compensation is locally provided by a directly coordinated oxygen vacancy. Using high-resolution synchrotron powder diffraction, it was verified that lead titanate remains tetragonal down to 12 K, exhibiting a c/a-ratio of 1.0721.Comment: 11 pages, 5 figures, accepted in Phys. Rev.

Safety, tumor trafficking and immunogenicity of chimeric antigen receptor (CAR)-T cells specific for TAG-72 in colorectal cancer.

BackgroundT cells engineered to express chimeric antigen receptors (CARs) have established efficacy in the treatment of B-cell malignancies, but their relevance in solid tumors remains undefined. Here we report results of the first human trials of CAR-T cells in the treatment of solid tumors performed in the 1990s.MethodsPatients with metastatic colorectal cancer (CRC) were treated in two phase 1 trials with first-generation retroviral transduced CAR-T cells targeting tumor-associated glycoprotein (TAG)-72 and including a CD3-zeta intracellular signaling domain (CART72 cells). In trial C-9701 and C-9702, CART72 cells were administered in escalating doses up to 1010 total cells; in trial C-9701 CART72 cells were administered by intravenous infusion. In trial C-9702, CART72 cells were administered via direct hepatic artery infusion in patients with colorectal liver metastases. In both trials, a brief course of interferon-alpha (IFN-α) was given with each CART72 infusion to upregulate expression of TAG-72.ResultsFourteen patients were enrolled in C-9701 and nine in C-9702. CART72 manufacturing success rate was 100% with an average transduction efficiency of 38%. Ten patients were treated in CC-9701 and 6 in CC-9702. Symptoms consistent with low-grade, cytokine release syndrome were observed in both trials without clear evidence of on target/off tumor toxicity. Detectable, but mostly short-term (≤14&nbsp;weeks), persistence of CART72 cells was observed in blood; one patient had CART72 cells detectable at 48&nbsp;weeks. Trafficking to tumor tissues was confirmed in a tumor biopsy from one of three patients. A subset of patients had 111Indium-labeled CART72 cells injected, and trafficking could be detected to liver, but T cells appeared largely excluded from large metastatic deposits. Tumor biomarkers carcinoembryonic antigen (CEA) and TAG-72 were measured in serum; there was a precipitous decline of TAG-72, but not CEA, in some patients due to induction of an interfering antibody to the TAG-72 binding domain of humanized CC49, reflecting an anti-CAR immune response. No radiologic tumor responses were observed.ConclusionThese findings demonstrate the relative safety of CART72 cells. The limited persistence supports the incorporation of co-stimulatory domains in the CAR design and the use of fully human CAR constructs to mitigate immunogenicity

Multiproxy study of anthropogenic and climatic changes in the last two millennia from a small mire in central Poland

The Żabieniec kettle-hole is the first peatland in central Poland analysed quantitatively with four biotic proxies (plant macrofossils, pollen, testate amoebae and chironomids) in order to reconstruct past environmental change. Palaeoecological data were supported by historical and archaeological records. We focused on autogenic vegetation change and human impact in relation to climatic effects. The aims of our study were: (a) to describe the development history of the mire during the last 2000 years, (b) to date and reconstruct the anthropogenic land-use changes, and (c) to discuss a possible climatic signal in the peat archive. The combination of proxies revealed dramatic shifts that took place in the peatland since the Roman Period. Żabieniec was a very wet telmatic habitat until ca AD 600. Then the water table declined and the site transformed into a Sphagnum-dominated mire. This dry shift took place mainly during the Early Medieval Period. Human impact was gradually increasing and it was particularly emphasized by deforestation since AD 1250 (beginning of the Late Medieval Period). Consequently, surface run-off and aeolian transport from the exposed soils caused the eutrophication of the mire. Furthermore, chironomids and testate amoebae reveal the beginning of a wet shift ca AD 1350. Openness considerably increased in the Late Medieval and the Modern Periods. The highest water table during the last 1000 years was recorded between AD 1500 and 1800. This wet event is connected with deforestation but it could be also associated with the Little Ice Age. Our study shows plant succession in the Żabieniec peatland, which can be explained with the recent landscape transformation. However, such changes are also possibly linked with the major climatic episodes during the last two millennia, such as the Medieval Warm Period and the Little Ice Age

A time study of physicians' work in a German university eye hospital to estimate unit costs.

BACKGROUND: Technical efficiency of hospital services is debated since performance has been heterogeneous. Staff time represents the main resource in patient care and its inappropriate allocation has been identified as a key factor of inefficiency. The aim of this study was to analyse the utilisation of physicians' work time stratified by staff groups, tasks and places of work. A further aim was to use these data to estimate resource use per unit of output. METHODS: A self-reporting work-sampling study was carried during 14-days at a University Eye Hospital. Staff costs of physicians per unit of output were calculated at the wards, the operating rooms and the outpatient unit. RESULTS: Forty per cent of total work time was spent in contact with the patient. Thirty per cent was spent with documentation tasks. Time spent with documentation tasks declined monotonically with increasing seniority of staff. Unit costs were 56 € per patient day at the wards, 77 € and 20 € per intervention at the operating rooms for inpatients and outpatients, respectively, and 33 € per contact at the outpatient unit. Substantial differences in resources directly dedicated to the patient were found between these locations. CONCLUSION: The presented data provide unprecedented units costs in inpatient Ophthalmology. Future research should focus on analysing factors that influence differences in time allocation, such as types of patients, organisation of care processes and composition of staff