Role of Genomics and RNA-seq in Studies of Fungal Virulence

Since its introduction in the last decade, massive parallel sequencing, or "next-generation sequencing", has revolutionized our access to genomic information, providing accurate data with increasingly higher yields and lower costs with respect to first-generation technology. Massive parallel sequencing of cDNA, or RNA-seq, is progressively replacing array-based technology as the method of choice for transcriptomics. This review describes some of the most recent applications of next-generation sequencing technology to the study of pathogenic fungi, including Candida, Aspergillus and Cryptococcusspecies. Several integrated approaches illustrate the power and accuracy of RNA-seq for studying the biology of human fungal pathogens. In addition, the lack of consistency in data analysis is discussed.Copyright: "It is fully accessible to all users at libraries and institutions that have purchased a SpringerLink license. If your article is published under one of our Open Access programs, it will be freely accessible to any user." Also, the journal is "Current Fungal Infection Reports", not the "Current Rheumatology" I used. This new journal was not on the list. Please add it.Have asked Research for journal to be added ([email protected]) 2012-11-23 J