Plasma Diagnostics and Magnetic Complexity of a Post-Flare Active Region with Hinode/XRT: Spatial and Temporal Evolution

Flares are localized phenomena in active regions, but the magnetic and plasma responses may propagate to a larger area. In this work we investigate the temporal evolution of a flare in an active region with particular attention to the morphological details, and to the temperature and emission measure diagnostics allowed by Hinode/XRT

Locating Hot Plasma in Small Flares using Spectroscopic Overlappogram Data from the Hinode EUV Imaging Spectrometer

One of the key processes associated with the “standard” flare model is chromospheric evaporation, a process during which plasma heated to high temperatures by energy deposition at the flare footpoints is driven upwards into the corona. Despite several decades of study, a number of open questions remain, including the relationship between plasma produced during this process and observations of earlier “superhot” plasma. The Extreme ultraviolet Imaging Spectrometer (EIS) onboard Hinode has a wide slot, which is often used as a flare trigger in the He II emission-line band. Once the intensity passes a threshold level, the study will switch to one focussed on the flaring region. However, when the intensity is not high enough to reach the flare trigger threshold, these datasets are then available during the entire flare period and provide high-cadence spectroscopic observations over a large field of view. We make use of data from two such studies of a C4.7 flare and a C1.6 flare to probe the relationship between hot Fe XXIV plasma and plasmas observed by the Reuven Ramaty High Energy Solar Spectroscopic Imager (RHESSI) and the X-ray Telescope (XRT) to track where the emission comes from and when it begins. The flare trigger slot data used in our analysis has one-minute cadence. Although the spatial and spectral information are merged in the wide-slot data, it is still possible to extract when the hot plasma appears, through the appearance of the Fe Xxiv spectral image. It is also possible to derive spectrally pure Fe XXIV light curves from the EIS data, and compare them with those derived from hard X-rays, enabling a full exploration of the evolution of hot emission. The Fe XXIV emission peaks just after the peak in the hard X-ray lightcurve; consistent with an origin in the evaporation of heated plasma following the transfer of energy to the lower atmosphere. A peak was also found for the C4.7 flare in the RHESSI peak temperature, which occurred before the hard X-rays peaked. This suggests that the first peak in hot-plasma emission is likely to be directly related to the energy-release process

Density diagnostics derIVed from the O IV and S IV intercombination lines observed by IRIS

The intensity of the \oiv~2s$^{2}$ 2p $^{2}$P-2s2p$^{2}$ $^{4}$P and \siv~3 s$^{2}$ 3p $^{2}$P- 3s 3p$^{2}$ $^{4}$ P intercombination lines around 1400~\AA~observed with the \textit{Interface Region Imaging Spectrograph} (IRIS) provide a useful tool to diagnose the electron number density ($N_\textrm{e}$) in the solar transition region plasma. We measure the electron number density in a variety of solar features observed by IRIS, including an active region (AR) loop, plage and brightening, and the ribbon of the 22-June-2015 M 6.5 class flare. By using the emissivity ratios of \oiv\ and \siv\ lines, we find that our observations are consistent with the emitting plasma being near isothermal (log$T$[K] $\approx$ 5) and iso-density ($N_\textrm{e}$ $\approx$~10$^{10.6}$ cm$^{-3}$) in the AR loop. Moreover, high electron number densities ($N_\textrm{e}$ $\approx$~10$^{13}$ cm$^{-3}$) are obtained during the impulsive phase of the flare by using the \siv\ line ratio. We note that the \siv\ lines provide a higher range of density sensitivity than the \oiv\ lines. Finally, we investigate the effects of high densities ($N_\textrm{e}$ $\gtrsim$ 10$^{11}$ cm$^{-3}$) on the ionization balance. In particular, the fractional ion abundances are found to be shifted towards lower temperatures for high densities compared to the low density case. We also explored the effects of a non-Maxwellian electron distribution on our diagnostic method.VP acknowledges support from the Isaac Newton Studentship, the Cambridge Trust, the IRIS team at Harvard-Smithsonian Centre for Astrophysics and the RS Newton Alumni Programme. GDZ and HEM acknowledge support from the STFC and the RS Newton Alumni Programme. JD acknowledges support from the RS Newton Alumni Programme. JD also acknowledges support from the Grant No. P209/12/1652 of the Grant Agency of the Czech Republic. AG acknowledges the in house research support provided by the Science and Technology Facilities Council. KR is supported by contract 8100002705 from Lockheed-Martin to SAO. IRIS is a NASA small explorer mission developed and operated by LMSAL with mission operations executed at NASA Ames Research Center and major contributions to downlink communications funded by the Norwegian Space Center (NSC, Norway) through an ESA PRODEX contract. AIA data are courtesy of NASA/SDO and the respective science teams. CHIANTI is a collaborative project involving researchers at the universities of Cambridge (UK), George Mason and Michigan (USA). ADAS is a project managed at the University of Strathclyde (UK) and funded through memberships universities and astrophysics and fusion laboratories in Europe and worldwide.This is the author accepted manuscript. It is currently under an indefinite embargo pending publication by EDP Sciences

The protease associated (PA) domain in ScpA from Streptococcus pyogenes plays a role in substrate recruitment

Annually, over 18 million disease cases and half a million deaths worldwide are estimated to be caused by Group A Streptococcus. ScpA (or C5a peptidase) is a well characterised member of the cell enveleope protease family, which possess a S8 subtilisin-like catalytic domain and a shared multi-domain architecture. ScpA cleaves complement factors C5a and C3a, impairing the function of these critical anaphylatoxins and disrupts complement-mediated innate immunity. Although the high resolution structure of ScpA is known, the details of how it recognises its substrate are only just emerging. Previous studies have identified a distant exosite on the 2nd fibronectin domain that plays an important role in recruitment via an interaction with the substrate core. Here, using a combination of solution NMR spectroscopy, mutagenesis with functional assays and computational approaches we identify a second exosite within the protease-associated (PA) domain. We propose a model in which the PA domain assists optimal delivery of the substrate's C terminus to the active site for cleavage

Dietary soy and meat proteins induce distinct physiological and gene expression changes in rats

This study reports on a comprehensive comparison of the effects of soy and meat proteins given at the recommended level on physiological markers of metabolic syndrome and the hepatic transcriptome. Male rats were fed semi-synthetic diets for 1 wk that differed only regarding protein source, with casein serving as reference. Body weight gain and adipose tissue mass were significantly reduced by soy but not meat proteins. The insulin resistance index was improved by soy, and to a lesser extent by meat proteins. Liver triacylglycerol contents were reduced by both protein sources, which coincided with increased plasma triacylglycerol concentrations. Both soy and meat proteins changed plasma amino acid patterns. The expression of 1571 and 1369 genes were altered by soy and meat proteins respectively. Functional classification revealed that lipid, energy and amino acid metabolic pathways, as well as insulin signaling pathways were regulated differently by soy and meat proteins. Several transcriptional regulators, including NFE2L2, ATF4, Srebf1 and Rictor were identified as potential key upstream regulators. These results suggest that soy and meat proteins induce distinct physiological and gene expression responses in rats and provide novel evidence and suggestions for the health effects of different protein sources in human diets

Does type 2 diabetes influence the risk of oesophageal adenocarcinoma?

Since hyperinsulinaemia may promote obesity-linked cancers, we compared type 2 diabetes prevalence among oesophageal adenocarcinoma (OAC) patients and population controls. Diabetes increased the risk of OAC (adjusted odds ratio 1.59, 95% confidence interval (CI) 1.04–2.43), although the risk was attenuated after further adjusting for body mass index (1.32, 95% CI 0.85–2.05)

X-ray Absorption and Reflection in Active Galactic Nuclei

X-ray spectroscopy offers an opportunity to study the complex mixture of emitting and absorbing components in the circumnuclear regions of active galactic nuclei, and to learn about the accretion process that fuels AGN and the feedback of material to their host galaxies. We describe the spectral signatures that may be studied and review the X-ray spectra and spectral variability of active galaxies, concentrating on progress from recent Chandra, XMM-Newton and Suzaku data for local type 1 AGN. We describe the evidence for absorption covering a wide range of column densities, ionization and dynamics, and discuss the growing evidence for partial-covering absorption from data at energies > 10 keV. Such absorption can also explain the observed X-ray spectral curvature and variability in AGN at lower energies and is likely an important factor in shaping the observed properties of this class of source. Consideration of self-consistent models for local AGN indicates that X-ray spectra likely comprise a combination of absorption and reflection effects from material originating within a few light days of the black hole as well as on larger scales. It is likely that AGN X-ray spectra may be strongly affected by the presence of disk-wind outflows that are expected in systems with high accretion rates, and we describe models that attempt to predict the effects of radiative transfer through such winds, and discuss the prospects for new data to test and address these ideas.Comment: Accepted for publication in the Astronomy and Astrophysics Review. 58 pages, 9 figures. V2 has fixed an error in footnote

Thyroid disease is a favorable prognostic factor in achieving sustained virologic response in chronic hepatitis C undergoing combination therapy: A nested case control study

<p>Abstract</p> <p>Background</p> <p>Interferon-α in combination with ribavirin is the current gold standard for treatment of chronic hepatitis C. It is unknown if the development of autoimmune thyroid disease (TD) during treatment confers an improved chance of achieving sustained virologic response. The aim of this study is to assess the chance of achieving sustained virologic response (SVR) in patients who developed TD during treatment when compared with those who did not.</p> <p>Methods</p> <p>We performed a tertiary hospital-based retrospective nested case-control analysis of 19 patients treated for hepatitis C who developed thyroid disease, and 76 controls (matched for age, weight, gender, cirrhosis and aminotransferase levels) who did not develop TD during treatment. Multivariate logistic-regression models were used to compare cases and controls.</p> <p>Results</p> <p>The development of TD was associated with a high likelihood of achieving SVR (odds ratio, 6.0; 95% confidence interval, 1.5 to 24.6) for the pooled group containing all genotypes. The likelihood of achieving SVR was increased in individuals with genotype 1 HCV infection who developed TD (odds ratio, 5.2; 95% confidence interval, 1.2 to 22.3), and all genotype 3 patients who developed TD achieved SVR.</p> <p>Conclusions</p> <p>Development of TD during treatment for hepatitis C infection is associated with a significantly increased chance of achieving SVR. The pathophysiogical mechanisms for this observation remain to be determined.</p> <p>Trial Registration</p> <p><it>The Australian New Zealand Clinical Trials Registry (ANZCTR)</it>: <a href="http://www.anzctr.org.au/ACTRB12610000830099.aspx">ACTRB12610000830099</a></p

Assessment of the effect of betaine on p16 and c-myc DNA methylation and mRNA expression in a chemical induced rat liver cancer model

<p>Abstract</p> <p>Background</p> <p>The development and progression of liver cancer may involve abnormal changes in DNA methylation, which lead to the activation of certain proto-oncogenes, such as <it>c-myc</it>, as well as the inactivation of certain tumor suppressors, such as <it>p16</it>. Betaine, as an active methyl-donor, maintains normal DNA methylation patterns. However, there are few investigations on the protective effect of betaine in hepatocarcinogenesis.</p> <p>Methods</p> <p>Four groups of rats were given diethylinitrosamine (DEN) and fed with AIN-93G diets supplemented with 0, 10, 20 or 40 g betaine/kg (model, 1%, 2%, and 4% betaine, respectively), while the control group, received no DEN, fed with AIN-93G diet. Eight or 15 weeks later, the expression of <it>p16 </it>and <it>c-myc </it>mRNA was examined by Real-time PCR (Q-PCR). The DNA methylation status within the <it>p16 </it>and <it>c-myc </it>promoter was analyzed using methylation-specific PCR.</p> <p>Results</p> <p>Compared with the model group, numbers and areas of glutathione S-transferase placental form (GST-p)-positive foci were decreased in the livers of the rats treated with betaine (<it>P < 0.05</it>). Although the frequency of <it>p16 </it>promoter methylation in livers of the four DEN-fed groups appeared to increase, there is no difference among these groups after 8 or 15 weeks (<it>P > 0.05</it>). Betaine supplementation attenuated the down-regulation of <it>p16 </it>and inhibited the up-regulation of <it>c-myc </it>induced by DEN in a dose-dependent manner (<it>P </it>< 0.01). Meanwhile, increases in levels of malondialdehyde (MDA) and glutathione S-transferase (GST) in model, 2% and 4% betaine groups were observed (<it>P < 0.05</it>). Finally, enhanced antioxidative capacity (T-AOC) was observed in both the 2% and 4% betaine groups.</p> <p>Conclusion</p> <p>Our data suggest that betaine attenuates DEN-induced damage in rat liver and reverses DEN-induced changes in mRNA levels.</p

GLUT 5 Is Not Over-Expressed in Breast Cancer Cells and Patient Breast Cancer Tissues

F18 2-Fluoro 2-deoxyglucose (FDG) has been the gold standard in positron emission tomography (PET) oncologic imaging since its introduction into the clinics several years ago. Seeking to complement FDG in the diagnosis of breast cancer using radio labeled fructose based analogs, we investigated the expression of the chief fructose transporter-GLUT 5 in breast cancer cells and human tissues. Our results indicate that GLUT 5 is not over-expressed in breast cancer tissues as assessed by an extensive immunohistochemistry study. RT-PCR studies showed that the GLUT 5 mRNA was present at minimal amounts in breast cancer cell lines. Further knocking down the expression of GLUT 5 in breast cancer cells using RNA interference did not affect the fructose uptake in these cell lines. Taken together these results are consistent with GLUT 5 not being essential for fructose uptake in breast cancer cells and tissues