Morphological and histochemical observations of the red jungle fowl tongue Gallus gallus

Morphological and histochemical study of the tongue of ten adult red jungle fowl (RJF), Gallus gallus were carried out at macroscopic and microscopic levels. The tongue was triangular with a wide dorsal and ventrolateral surface with median groove at the rostral part. Between the body and the roots appears a transverse row of the lingual conical papillae which was directed backwards. Behind the laryngeal cleft, there was a single row of pharyngeal papillae. The lingual mucosa showed parakeratinization, while there was a clearly recognizable keratinized band on the ventrolateral surface and the conical papillae. The cell cytoplasm of the medial group (MG) of the anterior lingual glands and the posterior glands contained large amounts of mucin compared with the lateral group (LG). The mucin of the lingual glands contained vicinal diol groups. Moreover, the sulphate containing glycoconjugates indicated in the MG and the posterior glands with a strong acid mucin reaction. Meanwhile, the LG of the anterior lingual glands exhibited carboxylated mucin with weak acid mucin reaction. In conclusion, the differences in the arrangement of the lingual and pharyngeal papillae in the RJF than that in other birds particularly domestic chicken may reflects the changes which occurred for the latter during domestication. The contents of mucins in the medial and lateral groups of the anterior lingual gland were varied, however, no differences histochemistry between the medial group and the posterior lingual gland were observed.Key words: Lingual salivary glands, mucin reaction, red jungle, tongue

Limitations of Flexural Crack Width in Simply Supported One-Way Ribbed Slab

The important consideration in design of multistory concrete buildings is reducing self-weight of the slabs. The system of one-way ribbed slab is one of the many systems chosen in order to reduce self-weight. This reduction occurs in concrete only for what causes high tensile stress in bottom fiber of concrete lead to cracking although the section is reinforced according to design specifications. Limitations of ACI 224R-01 2008 of maximum allowable crack width is 0.3 mm for members’ exposure to humidity, moist air, or soil. The experimental work in this research includes five simply supported reinforced concrete one-way ribbed slab with same dimensions and different in ratio of bottom steel reinforcement. The five specimens have reinforcement ratios of 0.0009, 0.0013, 0.0023, 0.0037, and 0.0059 respectively. The aim of this research is checking experimental crack width at design load with permissible limitations according to ACI 224R-01 2008. The experimental work and the theoretical calculations, for all the specimens, are cracked sections where the first crack appears at load less than design load. At design load the specimens with low and moderate reinforcement ratio 0.0009, 0.0013 and 0.0023 show crack width less than permissible value of 0.3 mm, while the specimens with high reinforcement ratio 0.0037 and 0.0059 show crack width more than permissible value of 0.3 mm, where the increases in width of crack causes reducing in the durability of members. At linear stage, the mid span deflection is decreasing as reinforcement ratio increase at same step of loading for all specimens. Finally, in the design of one-way ribbed slab, the emphasis is on checking the width of cracks according to codes permissible limitation and it is recommended to use low or moderate reinforcement ratio in this system of slabs

Time-series clustering of gene expression in irradiated and bystander fibroblasts: an application of FBPA clustering

<p>Abstract</p> <p>Background</p> <p>The radiation bystander effect is an important component of the overall biological response of tissues and organisms to ionizing radiation, but the signaling mechanisms between irradiated and non-irradiated bystander cells are not fully understood. In this study, we measured a time-series of gene expression after α-particle irradiation and applied the Feature Based Partitioning around medoids Algorithm (FBPA), a new clustering method suitable for sparse time series, to identify signaling modules that act in concert in the response to direct irradiation and bystander signaling. We compared our results with those of an alternate clustering method, Short Time series Expression Miner (STEM).</p> <p>Results</p> <p>While computational evaluations of both clustering results were similar, FBPA provided more biological insight. After irradiation, gene clusters were enriched for signal transduction, cell cycle/cell death and inflammation/immunity processes; but only FBPA separated clusters by function. In bystanders, gene clusters were enriched for cell communication/motility, signal transduction and inflammation processes; but biological functions did not separate as clearly with either clustering method as they did in irradiated samples. Network analysis confirmed p53 and NF-κB transcription factor-regulated gene clusters in irradiated and bystander cells and suggested novel regulators, such as KDM5B/JARID1B (lysine (K)-specific demethylase 5B) and HDACs (histone deacetylases), which could epigenetically coordinate gene expression after irradiation.</p> <p>Conclusions</p> <p>In this study, we have shown that a new time series clustering method, FBPA, can provide new leads to the mechanisms regulating the dynamic cellular response to radiation. The findings implicate epigenetic control of gene expression in addition to transcription factor networks.</p