137 research outputs found
Protein fractions and crude protein yield of cereal forages harvested at booting and dough stage of maturity
Hantavirus Brno loanvirus is highly specific to the common noctule bat (Nyctalus noctula) and widespread in Central Europe.
Bat-associated hantaviruses have been detected in Asia, Africa and Europe. Recently, a novel hantavirus (Brno loanvirus, BRNV) was identified in common noctule bats (Nyctalus noctula) in the Czech Republic, but nothing is known about its geographical range and prevalence. The objective of this study was to evaluate the distribution and host specificity of BRNV by testing bats from neighbouring countries Germany, Austria and Poland. One thousand forty-seven bats representing 21 species from Germany, 464 bats representing 18 species from Austria and 77 bats representing 12 species from Poland were screened by L segment broad-spectrum nested reverse transcription-polymerase chain reaction (RT-PCR) or by BRNV-specific real-time RT-PCR. Three common noctules from Germany, one common noctule from Austria and three common noctules from Poland were positive in the hantavirus RNA screening. Conventional RT-PCR and primer walking resulted in the amplification of partial L segment and (almost) complete S and M segment coding sequences for samples from Germany and partial L segment sequences for samples from Poland. Phylogenetic analysis of these nucleotide sequences showed highest similarity to BRNV from Czech Republic. The exclusive detection of BRNV in common noctules from different countries suggests high host specificity. The RNA detection rate in common noctules ranged between 1 of 207 (0.5%; Austria), 3 of 245 (1.2%; Germany) and 3 of 20 (15%; Poland). In conclusion, this study demonstrates a broader distribution of BRNV in common noctules in Central Europe, but at low to moderate prevalence. Additional studies are needed to prove the zoonotic potential of this hantavirus and evaluate its transmission within bat populations
The protective effects of osajin on ischemia/reperfusion injury to rat ovaries: biochemical and histopathological evaluation
An Integrated Pipeline for the Genome-Wide Analysis of Transcription Factor Binding Sites from ChIP-Seq
ChIP-Seq has become the standard method for genome-wide profiling DNA association
of transcription factors. To simplify analyzing and interpreting ChIP-Seq data,
which typically involves using multiple applications, we describe an integrated,
open source, R-based analysis pipeline. The pipeline addresses data input, peak
detection, sequence and motif analysis, visualization, and data export, and can
readily be extended via other R and Bioconductor packages. Using a standard
multicore computer, it can be used with datasets consisting of tens of thousands
of enriched regions. We demonstrate its effectiveness on published human
ChIP-Seq datasets for FOXA1, ER, CTCF and STAT1, where it detected co-occurring
motifs that were consistent with the literature but not detected by other
methods. Our pipeline provides the first complete set of Bioconductor tools for
sequence and motif analysis of ChIP-Seq and ChIP-chip data
Magnetic crystals and helical liquids in alkaline-earth fermionic gases
The joint action of a synthetic gauge potential and of atomic contact repulsion in a one-dimensional alkaline-earth(-like) fermionic gas with nuclear spin I leads to the existence of a hierarchy of fractional insulating and conducting states with intriguing properties. We unveil the existence and the features of those phases by means of both analytical bosonization techniques and numerical methods based on the density-matrix renormalization group algorithm. In particular, we show that the gapless phases can support helical modes, whereas the gapped states, which appear under certain conditions, are characterised both by density and magnetic order. Several distinct features emerge solely for spin I larger than 1/2, thus making their study with cold-atoms unique. We will finally argue that these states are related to the properties of an unconventional fractional quantum Hall effect in the thin-torus limit. The properties of this hierarchy of states can be experimentally studied in state-of-the-art cold-atom laboratories
Discovering Transcription Factor Binding Sites in Highly Repetitive Regions of Genomes with Multi-Read Analysis of ChIP-Seq Data
Chromatin immunoprecipitation followed by high-throughput sequencing (ChIP-seq) is rapidly replacing chromatin immunoprecipitation combined with genome-wide tiling array analysis (ChIP-chip) as the preferred approach for mapping transcription-factor binding sites and chromatin modifications. The state of the art for analyzing ChIP-seq data relies on using only reads that map uniquely to a relevant reference genome (uni-reads). This can lead to the omission of up to 30% of alignable reads. We describe a general approach for utilizing reads that map to multiple locations on the reference genome (multi-reads). Our approach is based on allocating multi-reads as fractional counts using a weighted alignment scheme. Using human STAT1 and mouse GATA1 ChIP-seq datasets, we illustrate that incorporation of multi-reads significantly increases sequencing depths, leads to detection of novel peaks that are not otherwise identifiable with uni-reads, and improves detection of peaks in mappable regions. We investigate various genome-wide characteristics of peaks detected only by utilization of multi-reads via computational experiments. Overall, peaks from multi-read analysis have similar characteristics to peaks that are identified by uni-reads except that the majority of them reside in segmental duplications. We further validate a number of GATA1 multi-read only peaks by independent quantitative real-time ChIP analysis and identify novel target genes of GATA1. These computational and experimental results establish that multi-reads can be of critical importance for studying transcription factor binding in highly repetitive regions of genomes with ChIP-seq experiments
Comparison of two methods of irrigant agitation in the removal of residual filling material in retreatment
Mechanical Preparation Showed Superior Shaping Ability than Manual Technique in Primary Molars - A Micro-Computed Tomography Study
Filling of simulated lateral canals with gutta-percha or thermoplastic polymer by warm vertical compaction
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