Genome Stability of Lyme Disease Spirochetes: Comparative Genomics of Borrelia burgdorferi Plasmids

Lyme disease is the most common tick-borne human illness in North America. In order to understand the molecular pathogenesis, natural diversity, population structure and epizootic spread of the North American Lyme agent, Borrelia burgdorferi sensu stricto, a much better understanding of the natural diversity of its genome will be required. Towards this end we present a comparative analysis of the nucleotide sequences of the numerous plasmids of B. burgdorferi isolates B31, N40, JD1 and 297. These strains were chosen because they include the three most commonly studied laboratory strains, and because they represent different major genetic lineages and so are informative regarding the genetic diversity and evolution of this organism. A unique feature of Borrelia genomes is that they carry a large number of linear and circular plasmids, and this work shows that strains N40, JD1, 297 and B31 carry related but non-identical sets of 16, 20, 19 and 21 plasmids, respectively, that comprise 33–40% of their genomes. We deduce that there are at least 28 plasmid compatibility types among the four strains. The B. burgdorferi ∼900 Kbp linear chromosomes are evolutionarily exceptionally stable, except for a short ≤20 Kbp plasmid-like section at the right end. A few of the plasmids, including the linear lp54 and circular cp26, are also very stable. We show here that the other plasmids, especially the linear ones, are considerably more variable. Nearly all of the linear plasmids have undergone one or more substantial inter-plasmid rearrangements since their last common ancestor. In spite of these rearrangements and differences in plasmid contents, the overall gene complement of the different isolates has remained relatively constant

Arthroscopic meniscal repair with an absorbable screw: results and surgical technique

The results of a new method for arthroscopic all-inside meniscus repair using a biodegradable cannulated screw (Clearfix meniscal screw) were assessed in a medium-term follow-up prospective study. The Clearfix meniscal screw system consists of delivery cannulae, screw driver, and screw implants. After tear debridement, a screw is located on the driver and passed through the cannula to the insertion site, holding the two sides of the tear together under linear compression. Forty-eight patients (48 repairs) with a mean age of 32.7 years were included in the study. Ligament stabilizing procedures were done in 39 patients (81%) who had anterior cruciate ligament deficient knees. Only longitudinal lesions in the red/red or red/white zone were repaired. Follow-up averaged 19 months, with a range from 12 to 48 months. Patients were evaluated using clinical examination, the "Orthopaedische Arbeitsgemeinschaft Knie (OAK)" knee evaluation scheme and magnetic resonance imaging (MRI). Criteria for clinical success included absence of joint-line tenderness, absence of swelling and a negative McMurray test. Postoperatively, there were no complications directly associated with the device. Twelve of 48 repaired menisci (25%) were considered failures according to the above-mentioned criteria. According to the OAK knee evaluation scheme, 38 patients (79%) had an excellent or good result. MRI, however, showed persisting grade III or IV lesions in 35 patients (73%). Analysis showed that age, length of tear, and simultaneous anterior cruciate ligament reconstruction did not affect the clinical outcome. In contrast, risk factors for failure of meniscus repair are chronicity of injury, location of tear more than 3 mm from the meniscosynovial junction and meniscus side (medial)