23 research outputs found
Characterization and Comparison of the Leukocyte Transcriptomes of Three Cattle Breeds
In this study, mRNA-Seq was used to characterize and compare the leukocyte transcriptomes from two taurine breeds (Holstein and Jersey), and one indicine breed (Cholistani). At the genomic level, we identified breed-specific base changes in protein coding regions. Among 7,793,425 coding bases, only 165 differed between Holstein and Jersey, and 3,383 (0.04%) differed between Holstein and Cholistani, 817 (25%) of which resulted in amino acid changes in 627 genes. At the transcriptional level, we assembled transcripts and estimated their abundances including those from more than 3,000 unannotated intergeneic regions. Differential gene expression analysis showed a high similarity between Holstein and Jersey, and a much greater difference between the taurine breeds and the indicine breed. We identified gene ontology pathways that were systematically altered, including the electron transport chain and immune response pathways that may contribute to different levels of heat tolerance and disease resistance in taurine and indicine breeds. At the post-transcriptional level, sequencing mRNA allowed us to identify a number of genes undergoing differential alternative splicing among different breeds. This study provided a high-resolution survey of the variation between bovine transcriptomes at different levels and may provide important biological insights into the phenotypic differentiation among cattle breeds
Dynamic adsorption of chromium ions onto natural and crosslinked chitosan membranes for wastewater treatment
Health sector reform and social determinants of health: building up theoretical and methodological interconnections to approach complex global challenges
Avaliação do risco de quedas em idosos atendidos em Unidade BĂĄsica de SaĂșde
A população mundial vem envelhecendo de forma abrupta, o que representa um grande desafio para os ĂłrgĂŁos competentes que, por sua vez, necessitam de novas polĂticas pĂșblicas de saĂșde, inclusive na prevenção de quedas. Este estudo objetivou avaliar o risco de quedas em idosos. Trata-se de um estudo epidemiolĂłgico de corte transversal com abordagem quantitativa, realizado em uma unidade de saĂșde da famĂlia. A amostra foi composta por 150 idosos avaliados de janeiro a abril de 2009. Para a coleta de dados, utilizou-se o Fall Risk Score, que foi analisado atravĂ©s do SPSS 17.0. Dos idosos avaliados 58,8% nĂŁo sofreram quedas, sendo que, dos idosos que caĂram (63 idosos), 71,4% sofreram de 1 a 2 quedas, citando como principal causa intrĂnseca a tontura/vertigem, enquanto que a extrĂnseca foi pisos escorregadios ou molhados. Conclui-se, portanto, que Ă© de grande relevĂąncia avaliar o risco de quedas em idosos, para que se medidas preventivas sejam tomadas, com o objetivo de maximizar a qualidade de vida