Pengujian Bahan Formulasi MsNPV ( Mythimna Separata Nuclear Polyhedrosis Virus) Terhadap Ulat Grayak Padi, Mythimna Separata Walker Di Lapangan

The objective of these studies were to observe efficiency material formulations of MsNPV and the influence to rice plantation on different locations (Indramayu (3 m above sea level), Sukabumi (400m above sea level) and Bogor (250 m above sea level) to larvae of rice army worm. This study used randomized block design with 4 treatments consisted of 3 formulation materials (talc, kaolin and gypsum) and control in five replications. Observations were done to life and death larvae on 5, 10, 15 days after inoculation and leaf damaged. Data were analysed with DMRT. Results from this study indicated that material talc formulation was more effective because all the material was dissolve as compare to material kaolin and gypsum formulations while the material were precipitated. Utilize these three material formulations on three different locations above sea level gave the same impact to the mortality army worm larvae and also to the plant damage

Formulasi Nuclear Polyhedrosis Virus (NPV) Untuk Mengendalikan Ulat Grayak Padi (Mythimna Separata Walker) Pada Tanaman Padi

Several methods can be used to control pest infestation, especially by the use of biological agents that do not contaminate environment. Virus as a biological agent has already been used to control army worm on some plants. Some benefits can be obtained when biological agents are used to control insect infestation. The objectives of these studies were to obtain: a method to produce MsNPV formulation from rice army worm; an effective and efficient method to pack and store MsNPV formulation as biological agent of rice army worm and an efficient application method of MsNPV formulation to control rice army worm. These studies were conducted at laboratory and green house in Bogor and rice field in Indramayu and Sukabumi, West Java during the planting season of 2005. The experiment consisted of several parts: production of suspension and MsNPV formulation from the larvae of ricearmy worm, packing and period storing of MsNPV formulation, observe the effect of MsNPV formulation on rice army worm in the rice fileds. Results from these studies show that the number of larvae used will effect production of suspension and formulation of MsNPV. The number of larvae used would increase the production of suspension and the value of formulation will also increase. While method of packing, period of time and location of storing showed that time period of 1; 2; and 3 months at different colour of plastic also location of storing refrigerator vs room temperature did not effect on mortality of larvae rice armyworm

Formulasi Nuclear Polyhedrosis Virus (NPV) untuk Mengendalikan Ulat Grayak Padi (Mythimna separata Walker) pada Tanaman Padi.

Several methods can be used to control pest infestation, especially by the use of biological agents that do not contaminate environment. Virus as a biological agent has already been used to control army worm on some plants. Some benefits can be obtained when biological agents are used to control insect infestation. The objectives of these studies were to obtain: 1) a method to produce MsNPV formulation from rice army worm; 2) an effective and efficient method to pack and store MsNPV formulation as biological agent of rice army worm and 3) an efficient application method of MsNPV formulation to control rice army worm. These studies were conducted at laboratory and green house in Bogor and rice field in Indramayu (Pantura) and Sukabumi, West Java during the planting season of 2005. The experiment consisted of several parts: 1) production of suspension and MsNPV formulation from the larvae of rice army worm, 2) packing and period storing of MsNPV formulation, 3) observe the effect of MsNPV formulation on rice army worm in the rice fileds. Results from these studies show that the number of larvae used will effect production of suspension and formulation of MsNPV. The number of larvae used would increase the production of suspension and the value of  formulation will also increase. While method of packing, period of time and location of storing showed that time period of 1; 2; and 3 months at different colour of plastic (blue and white) also location of storing refrigerator vs room temperature (cupboard and table) did not effect on mortality of larvae rice armyworm. KEY WORDS: Rice, army worm, NPV formulation

Pengujian Bahan Formulasi MsNPV (Mythimna separata Nuclear Polyhedrosis Virus) terhadap Ulat Grayak Padi, Mythimna separata Walker di Lapangan

The objective of these studies were to observe efficiency material formulations of MsNPV and the influence to rice plantation on different locations (Indramayu (3 m above sea level), Sukabumi (400m above sea level) and Bogor (250 m above sea level) to larvae of rice army worm. This study used randomized block design with 4 treatments consisted of 3 formulation materials (talc, kaolin and gypsum) and control in five replications. Observations were done to life and death larvae on 5, 10, 15 days after inoculation and leaf damaged. Data were analysed with DMRT. Results from this study indicated that material talc formulation was more effective because all the material was dissolve as compare to material kaolin and gypsum formulations while the material were precipitated. Utilize these three material formulations on three different locations above sea level gave the same impact to the mortality army worm larvae and also to the plant damage. KEY WORDS: Rice, army worm, material formulations of MsNP

MORPHOLOGICAL SPECIES VARIABILITY IN THE STEM-BORER GENUS Sdrpophaga, (LEPIDOPTERA: PYRALlDAE) ON GRAMINEOUS CROPS

The objective of this study is to observe variations between and within species using morphometry. Collections of Scirpophaga were carried out at rice fields in the Northern Coastal area of Java (Pantura): Kerawang, Cikampek, lndramayu, Cirebon, at sugar-cane plantations in Yogyakarta and Pasuruan and various gramineous crops in areas of South Bandung and Lampung from March 1998 to March 1999. Identification was done using morphology of adult characters. Variation within the population groups are recognized by taking measurement of parts of the head, for example measurement of the length and width of the head. Other characters used ill keying adult Lepidoptera to family are those of willg uenation. Also the morphology of male genitalia were used as main characters to differentiate species Results from this studies shownthat: a) Scirpophaga innotata. Measllrement of parts of the head from sample collected showed very little variation, ihier were mentioned as a group. Variation based on wing size, length and width of forewing can bedivided into two groups sizes; the large group size (950 - 1025 lI11n/100) and the smolt group size (850 - 860min/100). Variation based on the size of male gellitalia were more varied in ranging; between 20 - 26min/100 in length and 23 - 35 min/100 but they can be put into one group. b) Scirpophaga incertulas. Measurelllent of parts of the head show little variation in the scattered diagram distribution, and is considered as the same population group. Variation based on wing size, length and width of forewing collected from various places in Java indicated that populations of this insect does not indicate any differences in term of types of wing uenations and considered as similar populatious. Variation based on the size of male genitalia seemed to the same group in the scattered diagmm. c) Scirpophaga excerptalis Measurement of parts of the head(the length and width of the head) considerd as one population. Variation based on the size of nutle genitalia seemed to the smile group in the scattered diagrmn

Pengujian Bahan Formulasi MsNPV ( Mythimna separata Nuclear Polyhedrosis Virus) terhadap Ulat Grayak Padi, Mythimna separata Walker di Lapangan

The objective of these studies were to observe efficiency material formulations of MsNPV and the influence to rice plantation on different locations (Indramayu (3 m above sea level), Sukabumi (400m above sea level) and Bogor (250 m above sea level) to larvae of rice army worm. This study used randomized block design with 4 treatments consisted of 3 formulation materials (talc, kaolin and gypsum) and control in five replications. Observations were done to life and death larvae on 5, 10, 15 days after inoculation and leaf damaged. Data were analysed with DMRT. Results from this study indicated that material talc formulation was more effective because all the material was dissolve as compare to material kaolin and gypsum formulations while the material were precipitated. Utilize these three material formulations on three different locations above sea level gave the same impact to the mortality army worm larvae and also to the plant damage

Formulasi Nuclear Polyhedrosis Virus (NPV) untuk Mengendalikan Ulat Grayak Padi (Mythimna separata Walker) pada Tanaman Padi

Several methods can be used to control pest infestation, especially by the use of biological agents that do not contaminate environment. Virus as a biological agent has already been used to control army worm on some plants. Some benefits can be obtained when biological agents are used to control insect infestation. The objectives of these studies were to obtain: a method to produce MsNPV formulation from rice army worm; an effective and efficient method to pack and store MsNPV formulation as biological agent of rice army worm and an efficient application method of MsNPV formulation to control rice army worm. These studies were conducted at laboratory and green house in Bogor and rice field in Indramayu and Sukabumi, West Java during the planting season of 2005. The experiment consisted of several parts: production of suspension and MsNPV formulation from the larvae of ricearmy worm, packing and period storing of MsNPV formulation, observe the effect of MsNPV formulation on rice army worm in the rice fileds. Results from these studies show that the number of larvae used will effect production of suspension and formulation of MsNPV. The number of larvae used would increase the production of suspension and the value of formulation will also increase. While method of packing, period of time and location of storing showed that time period of 1; 2; and 3 months at different colour of plastic also location of storing refrigerator vs room temperature did not effect on mortality of larvae rice armyworm