Atypical aeromonas infection in cultured sea bass (Dicentrarchus labrax

Abstract The Black Sea, an inland sea, has contributed about 70% to the total fish production of Turkey for many years. Because of its low salinity and appropriate water temperature, both freshwater and marine fish such as salmon, trout, and sea bass can be cultured in the Black Sea. The aim of this study was to identify the etiological agent of mortality that occurred in sea bass in the Black Sea during July 2002. Bacteria isolated from kidneys and livers of infected fish were identified as Aeromonas salmonicida achromogenes according to morphological and biochemical characteristics, API 20NE results, and an agglutination test for A. salmonicida. The diseased fish had petechial hemorrhages on the bottom of the fins and lesions on the lateral and ventral sides of the body. Cumulative mortality was approximately 20%. This is the first report of A. salmonicida achromogenes associated with sea bass in Turkey. Introduction The genus Aeromonas comprises gram-negative, cytochrome oxidase-positive, facultatively-anaerobic rods. All but one of the Aeromonas species are motile. Aeromonas salmonicida is nonmotile and divided into four subspecies according to the Bergey's Manual of Systematic Bacteriology: salmonicida, achromonogenes, masoucida, and smithia. A

Kemo-mekanik çürük temizleme yöntemi ve geleneksel döner aletler ile temizlenen süt dişi dentin yüzeylerinin fiziko-kimyasal etkilerinin karılaştırılması

Amaç: Bu çalışma, süt dişi dentini yüzeyindeki çürüğün kemo-mekanik olarak Carisolv™ multimix jeli ve geleneksel döner aletler ile temizlenmesi sonrası fiziko-kimyasal etkilerini karşılaştırmak amacıyla yapıldı. Gereç ve Yöntem: Carisolv™ jeli ve döner aletlerle temizlenen dentin yüzey özellikleri taramalı elektron mikroskobuyla, pürüzlülükler ise yüzey profilometresi ile değerlendirildi. Bu amaçla, okluzal çürüklü çekilmiş 30 adet süt azı dişi dikey eksenine paralel olarak ikiye bölündü ve tek tarafındaki çürük lezyonu Carisolv™ jeli, diğeri ise geleneksel döner aletler ile temizlendi. Bulgular: Carisolv™ jeli ile temizlenen dentin yüzeylerinde, döner aletlere göre pürüzlülük değerlerinde azalma olduğu fakat bu azalmanın anlamlı olmadığı saptandı. Döner aletlerle temizlendikten sonra alınan SEM görüntüsünde, dentin yüzeylerindeki kollajen liflerin yer yer devamlılıklarını yitirdiği, smear tabakası ile dentin tübüllerinin tıkandığı ve uniform, düz bir yüzeyin oluştuğu gözlendi. Carisolv™ jeli ile temizledikten sonra SEM görüntüsünde dentin yüzeylerinde kollajen yapının devamlılığını sürdürdüğü, dentin tübüllerinde bir tıkanma olmadığı ve döner aletlerle temizlenen dentine göre yüzeyin daha pürüzlü ve poröz bir yapıda olduğu gözlendi. Sonuç: Bu sonuçlar ışığında Carisolv™ jeli ile çürük temizlemenin ardından yapılacak olan rezin bazlı restorasyonun tutuculuğunun, geleneksel döner aletlere göre daha iyi olacağı ileri sürülmektedir

Enteric Red Mouth Disease In Cultured Rainbow Trout (Oncorhynchus Mykiss) On The Black Sea Coast Of Turkey

Although Yersinia ruckeri, the etiological agent of the enteric red mouth disease (ERM), has been isolated from freshwater fish in several countries, there are few reports of its presence in cultured sea and brackish water fish. The aim of this study was to isolate and identify the etio- logical agent of disease outbreaks that occurred in rainbow trout cage farms on the Black Sea coast of Turkey in 2002. Clinical observation, biochemical, API 20 NE, and agglutination tests allowed the diagnosis of ERM caused by Y. ruckeri, which was successfully treated with med- icated feed (oxytetracycline at 75 mg per kg body weight per day for 10 days)

Atypical aeromonas infection in cultured sea bass in the Black Sea

The Black Sea, an inland sea, has contributed about 70% to the total fish production of Turkey for many years. Because of its low salinity and appropriate water temperature, both freshwater and marine fish such as salmon, trout, and sea bass can be cultured in the Black Sea. The aim of this study was to identify the etiological agent of mortality that occurred in sea bass in the Black Sea during July 2002. Bacteria isolated from kidneys and livers of infected fish were iden- tified as Aeromonas salmonicida achromogenes according to morphological and biochemical characteristics, API 20NE results, and an agglutination test for A. salmonicida. The diseased fish had petechial hemorrhages on the bottom of the fins and lesions on the lateral and ventral sides of the body. Cumulative mortality was approximately 20%. This is the first report of A. salmoni- cida achromogenes associated with sea bass in Turkey

Synthesis and characterization of a novel polyphosphazene and its application to biosensor in combination with a conducting polymer

WOS: 000338705700075An amperometric glucose biosensor was prepared successfully based on a conducting polymer, poly(4-(2,5-di(thiophen-2-y1)-1H-pyrrol-1-yl)benzenamine) (poly( SNS-NH2)) and a flexible hydrophilic polyphosphazene polymer, poly[(methoxyethoxy)ethoxy-co-3-formylphenoxy]phosphazene (PPA). Poly( SNS-NH2) was electrochemically polymerized on a graphite electrode to achieve a conducting immobilization matrix to improve the enzyme immobilization on the transducer surface. Moreover, to strengthen the immobilization, a polyphosphazene derivate bearing functional aldehyde group was designed, synthesized and used in the immobilization of glucose oxidase. Not only the amino groups in the structure of poly(SNS-NH2), but also the aldehyde groups in PPA were contributed to the covalent immobilization as well as the entrapping the biomolecules in PPA network during the immobilization process. This afforded an effective and long-life analysis of glucose. Amperometric measurements were conducted at 0.7V vs. Ag/AgCl in 50 mM sodium acetate buffer at pH 4.5. Km(app) (0.677mM), I-max (20.91 mu A), LOD (1.3 mu M) values were determined. Moreover, biosensor showed an extremely high sensitivity as 237.1 mu A mM(-1) cm(-2) owing to the newly synthesized and combined highly flexible hydrophilic polymeric immobilization matrix. Finally, the proposed biosensor was successfully applied for determination of glucose content in several beverages, successfully. (C) 2014 Elsevier B.V. All rights reserved

A histopathological study of Hexamitiasis in farmed rainbow trout (Oncorhynchus mykiss) fry in Turkey

The flagellated parasitic protozoan Hexamita salmonis were found in the upper intestine and pyloric caeca in large numbers in the farmed rainbow trout fry. Generally, infected fish fry showed significant level of mortality, exophthalmia and abdominal distension. Histopathologicaly, invasion of the pyloric caeca or upper intestine epithelium by the parasites and severe necrotizing of enteric mucosal epithelium and perforation of the serosal surface was observed. Inflammatory response was generally lacking in the gut wall, but large granulomatous lesions in some perforated area were found. In the histological sections, characteristic paired anterior nuclei of the parasites were easily recognized

Craniodentofacial Manifestations in a Rare Syndrome: Orofaciodigital Type IV (Mohr-Majewski Syndrome)

Background. The orofaciodigital syndromes (OFDS) are a heterogeneous group of syndromes that affect the face, oral cavity, and the digits. OFDS type IV (OMIM %258860) is rare and characterized by broad nasal root and tip, orbital hypertelorism or telecanthus, micrognathia, hypoplastic mandible, and low-set ears. Oral symptoms may include cleft lip, cleft or highly arched palate, bifid uvula, cleft or hypoplastic maxillary and mandibular alveolar ridge, oral frenula, lingual hamartoma, and absent or hypoplastic epiglottis. Dental anomalies are common and generally include disturbances in the number of teeth. Case Report. This report presents a six-year-old girl, referred with the chief complaint of missing teeth. She was diagnosed as having OFDS type IV based on clinical findings. Her parents reported three deceased children and two fetuses that had the same phenotype. She was the seventh child of consanguineous parents who were first cousins. Conclusion. This is a very rare syndrome. Many reported OFDS type IV cases have consanguineous parents, consistent with an autosomal recessive trait. Manifestation of cleft palate in the healthy sibling may be mild expression of the disorder or an unrelated isolated cleft

First Isolation of a Flavobacterium johnsoniae like Bacteria from Cultured Russian Sturgeon in Turkey

The aim of this study was to identify the causative agent responsible for low losses in cultured Russian sturgeon (Acipencer gueldenstaedtii) in Turkey. Two outbreaks occurred in the autumn of 2007 and 2008 after heavy rainfall accompanied by a sudden change in water temperature and increased suspended solids. The sturgeons displayed ulcerations, haemorrhage and superficial skin erosions especially on the ventral side including the pectoral and pelvic fins as reported in other studies. Affected fish were lethargic displayed excessive mucus secretion with skin lesions appearing as pale grey spots. Phenotypical characterizations were done according to standard protocols and supplemented with commercial APIZYM, API 20E and API 20NE kits. Sequencing of 16S rDNA PCR products were performed to genetically confirm the identity of the isolated organism

Changes in transferrin gene expression in sea bass (Dicentrarchus labrax) challenged with Vibrio anguillarum

Vibrio anguillarum expresses several virulence factors and causes hemorrhagic septicemia accompanied by serious losses in marine fish. Transferrin is a glycoprotein, also known as a multitasking protein, which is mainly synthesized by the liver. It has a fundamental role in the immune system. In the present study, the transferrin gene expression of sea bass (Dicentrarchus labrax) was investigated during an experimental infection with V. anguillarum. Fish samples were examined by hematological and serological methods as well as real-time polymerase chain reaction. The infection was performed via water. The sampled fish displayed vibriosis infection symptoms, both internally and externally. The transferrin saturation in the diseased fish serum decreased dramatically and the transferrin gene expression increased during the first 2 days; however, it decreased in the subsequent days