Mutation of Ser172 in Yeast β Tubulin Induces Defects in Microtubule Dynamics and Cell Division

Ser172 of β tubulin is an important residue that is mutated in a human brain disease and phosphorylated by the cyclin-dependent kinase Cdk1 in mammalian cells. To examine the role of this residue, we used the yeast S. cerevisiae as a model and produced two different mutations (S172A and S172E) of the conserved Ser172 in the yeast β tubulin Tub2p. The two mutants showed impaired cell growth on benomyl-containing medium and at cold temperatures, altered microtubule (MT) dynamics, and altered nucleus positioning and segregation. When cytoplasmic MT effectors Dyn1p or Kar9p were deleted in S172A and S172E mutants, cells were viable but presented increased ploidy. Furthermore, the two β tubulin mutations exhibited synthetic lethal interactions with Bik1p, Bim1p or Kar3p, which are effectors of cytoplasmic and spindle MTs. In the absence of Mad2p-dependent spindle checkpoint, both mutations are deleterious. These findings show the importance of Ser172 for the correct function of both cytoplasmic and spindle MTs and for normal cell division

Systematic errors in weather and climate models:Nature, origins, and ways forward

The fifth Workshop on Systematic Errors (WSE) in weather and climate models was hosted by Environment and Climate Change Canada (ECCC) on under the auspices of the Working Group on Numerical Experimentation (WGNE), jointly sponsored by the Commission of Atmospheric Sciences of the World Meteorological Organization (WMO) and the World Climate Research Programme (WCRP). This major event welcomed over 200 scientists from the weather and climate communities. The workshop primary goal was to increase the understanding of the nature and cause of systematic errors in numerical models across timescales. Out of 240 abstracts submitted to the workshop, 48 talks and 132 posters were presented

Functional states of kinetochores revealed by laser microsurgery and fluorescent speckle microscopy

The impact of mechanical forces on kinetochore motility was investigated using laser microsurgery and fluorescent speckle microscopy on kinetochores and associated microtubules during anaphase in crane fly spermatocytes. Kinetochores detached from their chromosomes moved at twice their normal speed, entering a motile state identified as “park.