64 research outputs found
Differential expression analysis of the broiler tracheal proteins responsible for the immune response and muscle contraction induced by high concentration of ammonia using iTRAQ-coupled 2D LC-MS/MS
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Fundamental studies of polymer filtration
This is the final report of a one-year, Laboratory Directed Research and Development (LDRD) project at Los Alamos National Laboratory (LANL). The objectives of this project were (1) to develop an enhanced fundamental understanding of the coordination chemistry of hazardous-metal-ion complexation with water-soluble metal-binding polymers, and (2) to exploit this knowledge to develop improved separations for analytical methods, metals processing, and waste treatment. We investigated features of water-soluble metal-binding polymers that affect their binding constants and selectivity for selected transition metal ions. We evaluated backbone polymers using light scattering and ultrafiltration techniques to determine the effect of pH and ionic strength on the molecular volume of the polymers. The backbone polymers were incrementally functionalized with a metal-binding ligand. A procedure and analytical method to determine the absolute level of functionalization was developed and the results correlated with the elemental analysis, viscosity, and molecular size
Measuring the realized niches of animals using stable isotopes: from rats to bears
© 2015 The Authors. Stable isotope analysis is a powerful method for estimating the impacts animals have on their environment (resource use), revealing their ecological niches. We demonstrated the use of a stable isotope mixing model for measuring the ecological niches of consumers. In particular, we used the model IsotopeR to estimate the resource use of two species with complex, omnivorous diets: invasive Norway rats from the Aleutian Islands, AK, and American black bears from Yosemite National Park, CA. Marginal posterior distributions for major food sources (for populations, groups and individuals) described the resource axes that partly define the realized niches of these omnivores. We used measures of these resource axes to inform resource management in the Aleutians and Yosemite. Results from our analyses confirm that coastal rats did not rely on marine birds on rat-infested islands in the Aleutians. Instead, rats foraged primarily on terrestrial plants and preferred amphipods when they were available. We also use stable isotopes to confirm that plants and acorns are the largest contributors to black bear nutrition in Yosemite and learned that female bears foraged for acorns and pine nuts more heavily than males. Although it is unclear if Norway rats can maintain viable populations in the Aleutians without access to marine-derived animal protein, results from our analyses suggest their dependence on such nutrients. In addition, sex-specific differences in foraging for high-fat acorns and pine nuts in Yosemite suggest black bear populations in the Sierra Nevada may be limited by the productivity and health of hard mast species. As demonstrated here, stable isotope analysis has wide applicability for investigating the resource use and ecological niches of animals. We anticipate and encourage its rapid development in this fundamental field of ecology
Dawn of E-Government – an Institutional Analysis of Seven Initiatives and Their Impact
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