Effect of Non-Coding RNA on Post-Transcriptional Gene Silencing of Alzheimer Disease

A large amount of hidden biological information is contained in the human genome, which is not expressed or revealed in the form of proteins; the usual end product form of gene expression. Instead, most of such information is in the form of non-coding RNAs (ncRNAs). ncRNAs correspond to genes that are transcribed, but do not get translated into proteins. This part of the genome was, till recently, considered as ‘junk’. The term ‘junk’ implied lack of any discernible function of these RNA. More than 98% of the human genomic size encompasses these non-coding RNAs. But, recent research has evidently brought out the indispensible contribution of non-coding RNA in controlling and regulating gene expression. ncRNA such as siRNAs and microRNAs have been reported to greatly help in causing post-transcriptional gene silencing (PTGS) in cells through RNA interference (RNAi) pathway. In this work, we have investigated the possibility of using siRNAs and microRNAs to aid in gene silencing of early onset Alzheimer’s disease genes. 
Alzheimer’s disease specific mutations and their corresponding positions in mRNA have been identified for six genes; Presenilin-1, Presenilin-2, APP (amyloid beta precursor protein), APBB3, BACE-1 and PSENEN. 

Small interfering RNAs (siRNAs) that can cause PTGS through RNA interference pathway have been designed. RNA analysis has been done to verify complementarity of antisense siRNA sequence with target mRNA sequence. Interaction studies have been done computationally between these antisense siRNA strands and seven Argonaute proteins. From the interaction studies, only one of the seven Argonaute proteins; 1Q8K, was found to have interaction with the siRNAs indicating the importance and uniqueness of this particular protein in RISC (RNA induced silencing complex). 

The interaction studies have been carried out for the microRNAs also. Out of the 700 mature human microRNAs collected, 394 microRNAs have been identified to show partial complementarity with their target sequence on PSEN-1 mRNA. Of these 394, five microRNAs have shown partial complementarity to early onset Alzheimer’s disease specific mutations in PSEN-1 mRNA. Interaction studies have been done between these microRNAs and Argonaute proteins. Thus, design, characterization and analysis of ncRNAs that contribute to post transcriptional gene silencing of Alzheimer’s disease have been achieved.
&#xa

A century of trends in adult human height

Being taller is associated with enhanced longevity, and higher education and earnings. We reanalysed 1472 population-based studies, with measurement of height on more than 18.6 million participants to estimate mean height for people born between 1896 and 1996 in 200 countries. The largest gain in adult height over the past century has occurred in South Korean women and Iranian men, who became 20.2 cm (95% credible interval 17.5-22.7) and 16.5 cm (13.3-19.7) taller, respectively. In contrast, there was little change in adult height in some sub-Saharan African countries and in South Asia over the century of analysis. The tallest people over these 100 years are men born in the Netherlands in the last quarter of 20th century, whose average heights surpassed 182.5 cm, and the shortest were women born in Guatemala in 1896 (140.3 cm; 135.8-144.8). The height differential between the tallest and shortest populations was 19-20 cm a century ago, and has remained the same for women and increased for men a century later despite substantial changes in the ranking of countries

Wavelet-based fundamental heart sound recognition method using morphological and interval features

Accurate and reliable recognition of fundamental heart sounds (FHSs) plays a significant role in automated analysis of heart sound (HS) patterns. This Letter presents an automated wavelet-based FHS recognition (WFHSR) method using morphological and interval features. The proposed method first performs the decomposition of phonocardiogram (PCG) signal using a synchrosqueezing wavelet transform to extract the HSs and suppresses the murmurs, low-frequency and high-frequency noises. The HS delineation (HSD) is presented using Shannnon energy envelope and amplitude-dependent thresholding rule. The FHS recognition (FHSR) is presented using interval, HS duration and envelope area features with a decision-rule algorithm. The performance of the method is evaluated on PASCAL HSs Challenge, PhysioNet/CinC HS Challenge, eGeneralMedical databases and real-time recorded PCG signals. Results show that the HSD approach achieves an average sensitivity (Se) of 98.87%, positive predictivity (Pp) of 97.50% with detection error rate of 3.67% for PCG signals with signal-to-noise ratio of 10 dB, and outperforms the existing HSD methods. The proposed FHSR method achieves a Se of 99.00%, Sp of 99.08% and overall accuracy of 99.04% on both normal and abnormal PCG signals. Evaluation results show that the proposed WFHSR method is able to accurately recognise the S1/S2 HSs in noisy real-world PCG recordings with murmurs and other abnormal sounds

The Oryza sativa no pollen (Osnop) gene plays a role in male gametophyte development and most likely encodes a C2-GRAM domain-containing protein

10.1007/s11103-005-2859-xPlant Molecular Biology576835-853PMBI