Atomic resolution structure of serine protease proteinase K at ambient temperature

Atomic resolution structures (beyond 1.20 ?) at ambient temperature, which is usually hampered by the radiation damage in synchrotron X-ray crystallography (SRX), will add to our understanding of the structure-function relationships of enzymes. Serial femtosecond crystallography (SFX) has attracted surging interest by providing a route to bypass such challenges. Yet the progress on atomic resolution analysis with SFX has been rather slow. In this report, we describe the 1.20 ? resolution structure of proteinase K using 13 keV photon energy. Hydrogen atoms, water molecules, and a number of alternative side-chain conformations have been resolved. The increase in the value of B-factor in SFX suggests that the residues and water molecules adjacent to active sites were flexible and exhibited dynamic motions at specific substrate-recognition sites. ? 2017 The Author(s).114Ysciescopu

Hydroxyethyl cellulose matrix applied to serial crystallography

Serial femtosecond crystallography (SFX) allows structures of proteins to be determined at room temperature with minimal radiation damage. A highly viscous matrix acts as a crystal carrier for serial sample loading at a low flow rate that enables the determination of the structure, while requiring consumption of less than 1 mg of the sample. However, a reliable and versatile carrier matrix for a wide variety of protein samples is still elusive. Here we introduce a hydroxyethyl cellulose-matrix carrier, to determine the structure of three proteins. The de novo structure determination of proteinase K from single-wavelength anomalous diffraction (SAD) by utilizing the anomalous signal of the praseodymium atom was demonstrated using 3,000 diffraction images. ? 2017 The Author(s).113Ysciescopu

Oil-free hyaluronic acid matrix for serial femtosecond crystallography

The grease matrix was originally introduced as a microcrystal-carrier for serial femtosecond crystallography and has been expanded to applications for various types of proteins, including membrane proteins. However, the grease-based matrix has limited application for oil-sensitive proteins. Here we introduce a grease-free, water-based hyaluronic acid matrix. Applications for proteinase K and lysozyme proteins were able to produce electron density maps at 2.3-angstrom resolution.open111011sciescopu

Spin-polarized Tunneling in Hybrid Metal-Semiconductor Magnetic Tunnel Junctions

We demonstrate efficient spin-polarized tunneling between a ferromagnetic metal and a ferromagnetic semiconductor with highly mismatched conductivities. This is indicated by a large tunneling magnetoresistance (up to 30%) at low temperatures in epitaxial magnetic tunnel junctions composed of a ferromagnetic metal (MnAs) and a ferromagnetic semiconductor (GaMnAs) separated by a nonmagnetic semiconductor (AlAs). Analysis of the current-voltage characteristics yields detailed information about the asymmetric tunnel barrier. The low temperature conductance-voltage characteristics show a zero bias anomaly and a V^1/2 dependence of the conductance, indicating a correlation gap in the density of states of GaMnAs. These experiments suggest that MnAs/AlAs heterostructures offer well characterized tunnel junctions for high efficiency spin injection into GaAs.Comment: 14 pages, submitted to Phys. Rev.

The Hyper Suprime-Cam SSP Survey: Overview and Survey Design

Hyper Suprime-Cam (HSC) is a wide-field imaging camera on the prime focus of the 8.2m Subaru telescope on the summit of Maunakea in Hawaii. A team of scientists from Japan, Taiwan and Princeton University is using HSC to carry out a 300-night multi-band imaging survey of the high-latitude sky. The survey includes three layers: the Wide layer will cover 1400 deg$^2$ in five broad bands ($grizy$), with a $5\,\sigma$ point-source depth of $r \approx 26$. The Deep layer covers a total of 26~deg$^2$ in four fields, going roughly a magnitude fainter, while the UltraDeep layer goes almost a magnitude fainter still in two pointings of HSC (a total of 3.5 deg$^2$). Here we describe the instrument, the science goals of the survey, and the survey strategy and data processing. This paper serves as an introduction to a special issue of the Publications of the Astronomical Society of Japan, which includes a large number of technical and scientific papers describing results from the early phases of this survey.Comment: 14 pages, 7 figures, 5 tables. Corrected for a typo in the coordinates of HSC-Wide spring equatorial field in Table

Measurement of Time-Dependent CP-Violating Asymmetries in B0 --> phi Ks0, K+ K- Ks0, and eta' Ks0 Decays

We present an improved measurement of CP-violation parameters in B0 --> phi Ks0, K+ K- Ks0, and eta' Ks0 decays based on a 140 fb-1 data sample collected at the Upsilon(4S) resonance with the Belle detector at the KEKB energy-asymmetric e+e- collider. One neutral B meson is fully reconstructed in one of the specified decay channels, and the flavor of the accompanying B meson is identified from its decay products. CP-violation parameters for each of the three modes are obtained from the asymmetries in the distributions of the proper-time intervals between the two B decays. We find that the observed CP asymmetry in the B0 --> phi Ks0 decay differs from the standard model (SM) expectation by 3.5 standard deviations, while the other cases are consistent with the SM.Comment: 10 pages, 4 postscript figures, submitted to Physical Review Letters, also contributed to the XXI International Symposium on Lepton and Photon Interactions at High Energies, Aug 11-16, 2003, Fermilab, Illinois US

Observation of Large CP Violation and Evidence for Direct CP Violation in B0-->pi+pi- Decays

We report the first observation of CP-violating asymmetries in B0 --> pi+pi- decays based on a 140 fb-1 data sample collected at the Upsilon(4S) resonance with the Belle detector at the KEKB asymmetric-energy e+e- collider. We reconstruct one neutral B meson as a B0 --> pi+pi- CP eigenstate and identify the flavor of the accompanying B meson from its decay products. We apply an unbinned maximum likelihood fit to the distribution of the time intervals between the two B meson decay points. The fit yields the CP-violating asymmetry amplitudes Apipi = +0.58+/-0.15(stat)+/-0.07(syst) and Spipi = -1.00+/-0.21(stat)+/-0.07(syst). We rule out the CP-conserving case, Apipi=Spipi=0, at a level of 5.2 standard deviations. We also find evidence for direct CP violation with a significance at or greater than 3.2 standard deviations for any Spipi value.Comment: 9 pages, 3 figure

Measurements of the DsJD_{sJ} resonance properties

We report measurements of the properties of the $D_{sJ}^+(2317)$ and $D_{sJ}^+(2457)$ resonances produced in continuum $e^+ e^-$ annihilation near $\sqrt{s}=10.6 \mathrm{GeV}$. The analysis is based on an $86.9 \mathrm{fb^{-1}}$ data sample collected %at and 60 MeV below the $\Upsilon(4S)$ resonance with with the Belle detector at KEKB. We determine the masses to be $M(D_{sJ}^+(2317)) = 2317.2 \pm 0.5(\mathrm{stat}) \pm 0.9(\mathrm{syst}) \mathrm{MeV}/c^2$ and $M(D_{sJ}^+(2457))=2456.5 \pm 1.3(\mathrm{stat}) \pm 1.3(\mathrm{syst}) \mathrm{MeV}/c^2$. We observe the radiative decay mode $D_{sJ}^+(2457) \to D_s^+ \gamma$ and the dipion decay mode $D_{sJ}^+(2457) \to D_s^+ \pi^+ \pi^-$, and determine their branching fractions. No corresponding decays are observed for the $D_{sJ}(2317)$ state. These results are consistent with the spin-parity assignments of $0^+$ for the $D_{sJ}(2317)$ and $1^+$ for the $D_{sJ}(2457)$.Comment: 5 pages, 6 figures; Added content, updated and paper submitted to PR

Evidence for B0->pi0pi0

We report evidence for the decay B0->pi0pi0. The analysis is based on a data sample of 152million BBbar pairs collected at the Upsilon(4s) resonance with the Belle detector at the KEKB e+e- storage ring. We find 25.6+9.3/-8.4(stat)+1.6/-1.4(syst) B0->pi0pi0 signal events with a significance of 3.4 standard deviations. We measure the branching fraction to be (1.7+-0.6(stat)+-0.2(syst))*10^{-6}.Comment: Submitted to PR