A study of tropical tropopause using MST radar

International audienceUsing the MST radar data of vertical wind, the characteristics of the tropical tropopause, following four different definitions, depending on 1) temperature lapse rate, 2) cold point, 3) convective outflow and 4) potential temperature lapse rate minimum, are studied. From the vertical wind data of the radar, the altitude profiles of temperature and horizontal divergence are derived, from which the tropopause levels corresponding to i) the lapse rate ii) cold point iii) convective outflow level and iv) potential temperature lapse rate minimum are determined. The convective outflow level and hence the convective tropopause altitude is determined, for the first time using the MST radar data. The tropopause altitudes and temperatures obtained following the four definitions are compared on a day-to-day basis for the summer and winter seasons. Winter and summer differences in the tropopause altitude and temperature are also studied. Keywords. Meteorology and atmospheric dynamics (convective process; middle atmosphere dynamics; tropical meteorology

Fresnel scatter revisited-comparison of 50 MHz radar and radiosondes in the Arctic, the Tropics and Antarctica

High-resolution radiosondes and calibrated radars operating close to 50 MHz, are used to examine the relationship between the strength of radar scatter and refractive index gradient. Three radars are used, in Kiruna in Arctic Sweden, at Gadanki in southern India and at the Swedish/Finnish base Wasa/Aboa in Queen Maud Land, Antarctica. Calibration is accomplished using the daily variation of galactic noise measured at each site. Proportionality between radar scatter strength and the square of the mean gradient of potential refractive index, M2, is found in the upper troposphere and lower stratosphere at all three sites, confirming previously reported results from many VHF radars. If the radar scatter is interpreted as Fresnel scatter, the constant of proportionality between radar scatter and M2 is found to be the same, within the calibration uncertainties, for all three radars. The radiosondes show evidence of distinct layering with sharp gradients, extending over 10s of kilometers horizontally, but the scatter is found to be two orders of magnitude weaker than would be expected from true Fresnel scatter from such layers. Using radar reflectivities resolved to a few 100 ms, we show that this is due to strong temporal variability in the scattering conditions, possibly due to undulations of the scattering layers. The constancy of the radar scatter – M2 relationship between the different sites suggests an unexpected uniformity in these perturbations between very different regions of the globe

Estimation of leaf area in turmeric (Curcuma longa L.) under two agroecological conditions

The total leaf area (TLA) at different stages of growth of tillers or clumps of turmeric (Curcuma longa) varieties raised under two agroecological conditions viz., in the open as a pure crop and in association with coconut, can be estimated using the regression equation TLA = bl nB + b2 L. The total leaf area of the tiller or clump can be computed by taking the linear measurements ('L' and 'B') of only one leaf i.e., median leaf of the tiller or those of the median leaf of the tiller having maximum number ofleaves, respectively, and counting the total number of leaves ('n') in the respective tiller or clump. Under both the ecological conditions studied and between the same, the regression coefficients were not homogenous over various stages of growth and for different varieties. Hence common mathematical formulae were not attempted to, with regard to varieties, age of the plant or ecological conditions. &nbsp

Estimation of leaf area in turmeric (Curcuma longa L.) under two agroecological conditions

The total leaf area (TLA) at different stages of growth of tillers or clumps of turmeric (Curcuma longa) varieties raised under two agroecological conditions viz., in the open as a pure crop and in association with coconut, can be estimated using the regression equation TLA = bl nB + b2 L. The total leaf area of the tiller or clump can be computed by taking the linear measurements ('L' and 'B') of only one leaf i.e., median leaf of the tiller or those of the median leaf of the tiller having maximum number ofleaves, respectively, and counting the total number of leaves ('n') in the respective tiller or clump. Under both the ecological conditions studied and between the same, the regression coefficients were not homogenous over various stages of growth and for different varieties. Hence common mathematical formulae were not attempted to, with regard to varieties, age of the plant or ecological conditions. &nbsp

Lidar Observations of aerosol layers just below the tropopause level during IFP-INDOEX

A lidar system has been used at Gadanki (13.5º, 79.2ºE) to study the characteristics of aerosol layer (cloud) occurring just below the tropical tropopause. The preliminary results of the lidar observations indicate that the cloud occurs ~ 2 km below the tropopause. The top and bottom edges of the cloud have propensity for ice crystal presence with liquid droplets/ vapours in-between. The clouds show temporal fluctuations (in their backscattering ratio) with temporal scales of the order of 30–90 min

Optimum dose of nitrogen and potassium for ginger in Wynad, Kerala

Study on nutrient requirement of ginger in Wynad, Kerala showed the positive effect of higher, doses of Nand K on the yield. Among the 16 levels of Nand K, 3 combinations viz., 150 kg N, 50 kg K; 150 kg N, 100 kg K and 75 kg N, 150 kg K ha-1 were found to be significantly superior with respect to yield. Among the vegetative characters, plant height was found to be significantly influenced by nitrogen. The optimum dose of Nand K derived from the quadratic equation was 144 kg and 109 kg ha-1 respectively. &nbsp

Fine-tuning the spike: role of the nature and topology of the glycan shield in the structure and dynamics of the SARS-CoV-2 S

The dense glycan shield is an essential feature of the SARS-CoV-2 spike (S) architecture, key to immune evasion and to the activation of the prefusion conformation. Recent studies indicate that the occupancy and structures of the SARS-CoV-2 S glycans depend not only on the nature of the host cell, but also on the structural stability of the trimer; a point that raises important questions about the relative competence of different glycoforms. Moreover, the functional role of the glycan shield in the SARS-CoV-2 pathogenesis suggests that the evolution of the sites of glycosylation is potentially intertwined with the evolution of the protein sequence to affect optimal activity. Our results from multi-microsecond molecular dynamics simulations indicate that the type of glycosylation at N234, N165 and N343 greatly affects the stability of the receptor binding domain (RBD) open conformation, and thus its exposure and accessibility. Furthermore, our results suggest that the loss of glycosylation at N370, a newly acquired modification in the SARS-CoV-2 S glycan shield's topology, may have contributed to increase the SARS-CoV-2 infectivity as we find that N -glycosylation at N370 stabilizes the closed RBD conformation by binding a specific cleft on the RBD surface. We discuss how the absence of the N370 glycan in the SARS-CoV-2 S frees the RBD glycan binding cleft, which becomes available to bind cell-surface glycans, and potentially increases host cell surface localization. The N -glycans structures affect the mechanistic properties of the SARS-CoV-2 S, fine-tuning the glycoprotein. The evolution of the glycan shield led to the loss of N370 glycosylation in SARS-CoV-2 S, where the RBD cleft can bind host-cell glycans

Utilisation of an operative difficulty grading scale for laparoscopic cholecystectomy

Background A reliable system for grading operative difficulty of laparoscopic cholecystectomy would standardise description of findings and reporting of outcomes. The aim of this study was to validate a difficulty grading system (Nassar scale), testing its applicability and consistency in two large prospective datasets. Methods Patient and disease-related variables and 30-day outcomes were identified in two prospective cholecystectomy databases: the multi-centre prospective cohort of 8820 patients from the recent CholeS Study and the single-surgeon series containing 4089 patients. Operative data and patient outcomes were correlated with Nassar operative difficultly scale, using Kendall’s tau for dichotomous variables, or Jonckheere–Terpstra tests for continuous variables. A ROC curve analysis was performed, to quantify the predictive accuracy of the scale for each outcome, with continuous outcomes dichotomised, prior to analysis. Results A higher operative difficulty grade was consistently associated with worse outcomes for the patients in both the reference and CholeS cohorts. The median length of stay increased from 0 to 4 days, and the 30-day complication rate from 7.6 to 24.4% as the difficulty grade increased from 1 to 4/5 (both p < 0.001). In the CholeS cohort, a higher difficulty grade was found to be most strongly associated with conversion to open and 30-day mortality (AUROC = 0.903, 0.822, respectively). On multivariable analysis, the Nassar operative difficultly scale was found to be a significant independent predictor of operative duration, conversion to open surgery, 30-day complications and 30-day reintervention (all p < 0.001). Conclusion We have shown that an operative difficulty scale can standardise the description of operative findings by multiple grades of surgeons to facilitate audit, training assessment and research. It provides a tool for reporting operative findings, disease severity and technical difficulty and can be utilised in future research to reliably compare outcomes according to case mix and intra-operative difficulty