638 research outputs found
Structure and behaviour of the sperm terminal filament
Light- and electron-microscopic observations of Ciona and Lytechinus spermatozoa show a thin terminal filament at the distal end. The terminal filament is 5-6 microns long and contains the two central microtubules and a variable number of A-tubule extensions of the peripheral doublet microtubules. The transition from the 9 + 2 region to the terminal filament is tapered more gradually in Lytechinus than in Ciona. Photographs of the movement of beating spermatozoa do not show any obvious discontinuity in curvature at the transition region. Bends are propagated smoothly off the end of the flagellum with no decrease in curvature. However, spermatozoa in which the terminal filament has been removed show a clear 'end effect'. This end effect involves a rapid unbending of bends that have reached the distal end of the flagellum. Computer simulations of flagellar models lacking a terminal filament show a similar end effect. Addition of a terminal filament to the end of the computer model can eliminate the end effect. Realistic bending behaviour of the model is obtained by using a terminal filament with a tapered elastic bending resistance in the basal portion of the terminal filament and a value of 0.03 x 10^(9) pN nm^2 in the remainder of the terminal filament. This leads to estimates of 0.01 x 10^(9) pN nm^2 for the elastic bending resistance of an individual microtubule, and 0.2 x 10^(9) pN nm^2 for the elastic bending resistance of the 9 + 2 region of the flagellum. An improvement in propulsive effectiveness by addition of a terminal filament remains to be demonstrated
Electrical and magnetic properties of ion-exchangeable layered ruthenates
ArticleJournal of Solid State Chemistry. 177(12):4542-4545 (2004)journal articl
In Vivo oocyte maturation and ovulation in females and spermiation in males of hybrid sturgeon, bester
Bester, a hybrid sturgeon (Huso huso L. females ´ Acipenser ruthenus L. males), neither spermiate nor ovulate in the captivity. Thirteen-year-old adult male and female bester were injected with LH-RHa (0.1-0.3 mg/kg B.W.) intramuscularly and spawning status of the treated fish was checked 24-48 hours later. Additionally, changes in serum levels of DHP in both the responded and the non-responded individuals were monitored 0, 1, 3, 6, 9, 12, 24, 48 and 72 hours after treatment. Between 70-100 percent of the LR-RHa injected males individuals, and 11-40 percent of the females spawned 24-48 hours after the treatment; the rest did not respond to the injection. In the responded males, 3-9 hours after the treatment, serum levels of DHP increased; while in the females it occurred first after 12-24 hours. In contrast, during the same period, serum levels of DHP remained low in all non-responded individuals. The present study indicated that using 0.1-1.3 mg/kg B. W. of LH-RHa can induce oocyte maturation and ovulation in the females and spermiation in the males cultured bester. The results suggested additionally DHP as an appropriated steroid that could be used in the final maturation stage of the gonads
Retrovirology: 3 at age 2
Retrovirology announces new editorial board members and reprises progress over the first two years of publishing
Applicability of transesophageal imaging of the flow convergence region for evaluating patient ductus arteriosus
Lineage-associated tracts defining the anatomy of the Drosophila first instar larval brain
AbstractFixed lineages derived from unique, genetically specified neuroblasts form the anatomical building blocks of the Drosophila brain. Neurons belonging to the same lineage project their axons in a common tract, which is labeled by neuronal markers. In this paper, we present a detailed atlas of the lineage-associated tracts forming the brain of the early Drosophila larva, based on the use of global markers (anti-Neuroglian, anti-Neurotactin, inscuteable-Gal4>UAS-chRFP-Tub) and lineage-specific reporters. We describe 68 discrete fiber bundles that contain axons of one lineage or pairs/small sets of adjacent lineages. Bundles enter the neuropil at invariant locations, the lineage tract entry portals. Within the neuropil, these fiber bundles form larger fascicles that can be classified, by their main orientation, into longitudinal, transverse, and vertical (ascending/descending) fascicles. We present 3D digital models of lineage tract entry portals and neuropil fascicles, set into relationship to commonly used, easily recognizable reference structures such as the mushroom body, the antennal lobe, the optic lobe, and the Fasciclin II-positive fiber bundles that connect the brain and ventral nerve cord. Correspondences and differences between early larval tract anatomy and the previously described late larval and adult lineage patterns are highlighted. Our L1 neuro-anatomical atlas of lineages constitutes an essential step towards following morphologically defined lineages to the neuroblasts of the early embryo, which will ultimately make it possible to link the structure and connectivity of a lineage to the expression of genes in the particular neuroblast that gives rise to that lineage. Furthermore, the L1 atlas will be important for a host of ongoing work that attempts to reconstruct neuronal connectivity at the level of resolution of single neurons and their synapses
HIV-1 nef suppression by virally encoded microRNA
BACKGROUND: MicroRNAs (miRNAs) are 21~25-nucleotides (nt) long and interact with mRNAs to trigger either translational repression or RNA cleavage through RNA interference (RNAi), depending on the degree of complementarity with the target mRNAs. Our recent study has shown that HIV-1 nef dsRNA from AIDS patients who are long-term non-progressors (LTNPs) inhibited the transcription of HIV-1. RESULTS: Here, we show the possibility that nef-derived miRNAs are produced in HIV-1 persistently infected cells. Furthermore, nef short hairpin RNA (shRNA) that corresponded to a predicted nef miRNA (~25 nt, miR-N367) can block HIV-1 Nef expression in vitro and the suppression by shRNA/miR-N367 would be related with low viremia in an LTNP (15-2-2). In the 15-2-2 model mice, the weight loss, which may be rendered by nef was also inhibited by shRNA/miR-N367 corresponding to suppression of nef expression in vivo. CONCLUSIONS: These data suggest that nef/U3 miRNAs produced in HIV-1-infected cells may suppress both Nef function and HIV-1 virulence through the RNAi pathway
Discerning the origins of the Negritos, first Sundaland people: Deep divergence and archaic admixture
Human presence in Southeast Asia dates back to at least 40,000 years ago, when the current islands formed a continental shelf called Sundaland. In the Philippine Islands, Peninsular Malaysia, and Andaman Islands, there exist indigenous groups collectively called Negritos whose ancestry can be traced to the “First Sundaland People.” To understand the relationship between these Negrito groups and their demographic histories, we generated genome-wide single nucleotide polymorphism data in the Philippine Negritos and compared them with existing data from other populations. Phylogenetic tree analyses show that Negritos are basal to other East and Southeast Asians, and that they diverged from West Eurasians at least 38,000 years ago. We also found relatively high traces of Denisovan admixture in the Philippine Negritos, but not in the Malaysian and Andamanese groups, suggesting independent introgression and/or parallel losses involving Denisovan introgressed regions. Shared genetic loci between all three Negrito groups could be related to skin pigmentation, height, facial morphology and malarial resistance. These results show the unique status of Negrito groups as descended from the First Sundaland People
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A visual pathway for skylight polarization processing in Drosophila
Many insects use patterns of polarized light in the sky to orient and navigate. Here, we functionally characterize neural circuitry in the fruit fly, Drosophila melanogaster, that conveys polarized light signals from the eye to the central complex, a brain region essential for the fly’s sense of direction. Neurons tuned to the angle of polarization of ultraviolet light are found throughout the anterior visual pathway, connecting the optic lobes with the central complex via the anterior optic tubercle and bulb, in a homologous organization to the ‘sky compass’ pathways described in other insects. We detail how a consistent, map-like organization of neural tunings in the peripheral visual system is transformed into a reduced representation suited to flexible processing in the central brain. This study identifies computational motifs of the transformation, enabling mechanistic comparisons of multisensory integration and central processing for navigation in the brains of insects
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