1,144 research outputs found
Characterization and modeling of strain assisted diffusion in an epoxy adhesive layer
AbstractIn this paper a coupled model for strain-assisted diffusion is derived from the basic principles of continuum mechanics and thermodynamics, and material properties characterized using diffusion experiments. The proposed methodology constitutes a significant step toward modeling the synergistic bond degradation mechanism at the bonded interface between a Fiber Reinforced Polymer (FRP) and a substrate, and for predicting debond initiation and propagation along the interface in the presence of a diffusing penetrant at the crack tip and at elevated temperatures. It is now well-known that Fick’s law is frequently inadequate for describing moisture diffusion in polymers and polymer composites. Non-Fickian or anomalous diffusion is likely to occur when a polymer is subjected to external stresses and strains, as well as elevated temperatures and humidity. In this paper, a modeling methodology based on the basic principles of continuum mechanics and thermodynamics is developed which allows characterization of the combined effects of temperature, humidity, and strain on diffusion coefficients as well as on moisture saturation level, from moisture weight gain data. For tractability, the diffusion governing equations are simplified for the special case of 1-D diffusion subjected to uniaxial strain and a uniform strain gradient. A novel test specimen that introduces a uniform strain gradient is developed, and diffusion test data for an epoxy-based primer/adhesive are presented and employed for complete characterization of material constants used in the model
The potential of radar-based ensemble forecasts for flash-flood early warning in the southern Swiss Alps
This study explores the limits of radar-based forecasting for hydrological runoff prediction. Two novel radar-based ensemble forecasting chains for flash-flood early warning are investigated in three catchments in the southern Swiss Alps and set in relation to deterministic discharge forecasts for the same catchments. The first radar-based ensemble forecasting chain is driven by NORA (Nowcasting of Orographic Rainfall by means of Analogues), an analogue-based heuristic nowcasting system to predict orographic rainfall for the following eight hours. The second ensemble forecasting system evaluated is REAL-C2, where the numerical weather prediction COSMO-2 is initialised with 25 different initial conditions derived from a four-day nowcast with the radar ensemble REAL. Additionally, three deterministic forecasting chains were analysed. The performance of these five flash-flood forecasting systems was analysed for 1389 h between June 2007 and December 2010 for which NORA forecasts were issued, due to the presence of orographic forcing. A clear preference was found for the ensemble approach. Discharge forecasts perform better when forced by NORA and REAL-C2 rather then by deterministic weather radar data. Moreover, it was observed that using an ensemble of initial conditions at the forecast initialisation, as in REAL-C2, significantly improved the forecast skill. These forecasts also perform better then forecasts forced by ensemble rainfall forecasts (NORA) initialised form a single initial condition of the hydrological model. Thus the best results were obtained with the REAL-C2 forecasting chain. However, for regions where REAL cannot be produced, NORA might be an option for forecasting events triggered by orographic precipitation
Evidence for a Massive Protocluster in S255N
S255N is a luminous far-infrared source that contains many indications of
active star formation but lacks a prominent near-infrared stellar cluster. We
present mid-infrared through radio observations aimed at exploring the
evolutionary state of this region. Our observations include 1.3mm continuum and
spectral line data from the Submillimeter Array, VLA 3.6cm continuum and 1.3cm
water maser data, and multicolor IRAC images from the Spitzer Space Telescope.
The cometary morphology of the previously-known UCHII region G192.584-0.041 is
clearly revealed in our sensitive, multi-configuration 3.6cm images. The 1.3mm
continuum emission has been resolved into three compact cores, all of which are
dominated by dust emission and have radii < 7000AU. The mass estimates for
these cores range from 6 to 35 Msun. The centroid of the brightest dust core
(SMA1) is offset by 1.1'' (2800 AU) from the peak of the cometary UCHII region
and exhibits the strongest HC3N, CN, and DCN line emission in the region. SMA1
also exhibits compact CH3OH, SiO, and H2CO emission and likely contains a young
hot core. We find spatial and kinematic evidence that SMA1 may contain further
multiplicity, with one of the components coincident with a newly-detected H2O
maser. There are no mid-infrared point source counterparts to any of the dust
cores, further suggesting an early evolutionary phase for these objects. The
dominant mid-infrared emission is a diffuse, broadband component that traces
the surface of the cometary UCHII region but is obscured by foreground material
on its southern edge. An additional 4.5 micron linear feature emanating to the
northeast of SMA1 is aligned with a cluster of methanol masers and likely
traces a outflow from a protostar within SMA1. Our observations provide direct
evidence that S255N is forming a cluster of intermediate to high-mass stars.Comment: 34 pages, 11 figures, accepted for publication in The Astronomical
Journa
Does Greater Low Frequency EEG Activity in Normal Immaturity and in Children with Epilepsy Arise in the Same Neuronal Network?
Greater low frequency power (<8Hz) in the electroencephalogram (EEG) at rest is normal in the immature developing brain of children when compared to adults. Children with epilepsy also have greater low frequency interictal resting EEG activity. Whether these power elevations reflect brain immaturity due to a developmental lag or the underlying epileptic pathophysiology is unclear. The present study addresses this question by analyzing spectral EEG topographies and sources for normally developing children and children with epilepsy. We first compared the resting EEG of healthy children to that of healthy adults to isolate effects related to normal brain immaturity. Next, we compared the EEG from 10 children with generalized cryptogenic epilepsy to the EEG of 24 healthy children to isolate effects related to epilepsy. Spectral analysis revealed that global low (delta: 1-3Hz, theta: 4-7Hz), medium (alpha: 8-12Hz) and high (beta: 13-25Hz) frequency EEG activity was greater in children without epilepsy compared to adults, and even further elevated for children with epilepsy. Topographical and tomographic EEG analyses showed that normal immaturity corresponded to greater delta and theta activity at fronto-central scalp and brain regions, respectively. In contrast, the epilepsy-related activity elevations were predominantly in the alpha band at parieto-occipital electrodes and brain regions, respectively. We conclude that lower frequency activity can be a sign of normal brain immaturity or brain pathology depending on the specific topography and frequency of the oscillating neuronal networ
Matrix metalloproteinase-9 activity and a downregulated Hedgehog pathway impair blood-brain barrier function in an <i>in vitro</i> model of CNS tuberculosis
Central nervous system tuberculosis (CNS TB) has a high mortality and morbidity associated with severe inflammation. The blood-brain barrier (BBB) protects the brain from inflammation but the mechanisms causing BBB damage in CNS TB are uncharacterized. We demonstrate that Mycobacterium tuberculosis (Mtb) causes breakdown of type IV collagen and decreases tight junction protein (TJP) expression in a co-culture model of the BBB. This increases permeability, surface expression of endothelial adhesion molecules and leukocyte transmigration. TJP breakdown was driven by Mtb-dependent secretion of matrix metalloproteinase (MMP)-9. TJP expression is regulated by Sonic hedgehog (Shh) through transcription factor Gli-1. In our model, the hedgehog pathway was downregulated by Mtb-stimulation, but Shh levels in astrocytes were unchanged. However, Scube2, a glycoprotein regulating astrocyte Shh release was decreased, inhibiting Shh delivery to brain endothelial cells. Activation of the hedgehog pathway by addition of a Smoothened agonist or by addition of exogenous Shh, or neutralizing MMP-9 activity, decreased permeability and increased TJP expression in the Mtb-stimulated BBB co-cultures. In summary, the BBB is disrupted by downregulation of the Shh pathway and breakdown of TJPs, secondary to increased MMP-9 activity which suggests that these pathways are potential novel targets for host directed therapy in CNS TB
Transcriptome and translatome co-evolution in mammals.
Gene-expression programs define shared and species-specific phenotypes, but their evolution remains largely uncharacterized beyond the transcriptome layer <sup>1</sup> . Here we report an analysis of the co-evolution of translatomes and transcriptomes using ribosome-profiling and matched RNA-sequencing data for three organs (brain, liver and testis) in five mammals (human, macaque, mouse, opossum and platypus) and a bird (chicken). Our within-species analyses reveal that translational regulation is widespread in the different organs, in particular across the spermatogenic cell types of the testis. The between-species divergence in gene expression is around 20% lower at the translatome layer than at the transcriptome layer owing to extensive buffering between the expression layers, which especially preserved old, essential and housekeeping genes. Translational upregulation specifically counterbalanced global dosage reductions during the evolution of sex chromosomes and the effects of meiotic sex-chromosome inactivation during spermatogenesis. Despite the overall prevalence of buffering, some genes evolved faster at the translatome layer-potentially indicating adaptive changes in expression; testis tissue shows the highest fraction of such genes. Further analyses incorporating mass spectrometry proteomics data establish that the co-evolution of transcriptomes and translatomes is reflected at the proteome layer. Together, our work uncovers co-evolutionary patterns and associated selective forces across the expression layers, and provides a resource for understanding their interplay in mammalian organs
Clade-level Spatial Modelling of HPAI H5N1 Dynamics in the Mekong Region Reveals New Patterns and Associations with Agro-Ecological Factors.
The highly pathogenic avian influenza (HPAI) H5N1 virus has been circulating in Asia since 2003 and diversified into several genetic lineages, or clades. Although the spatial distribution of its outbreaks was extensively studied, differences in clades were never previously taken into account. We developed models to quantify associations over time and space between different HPAI H5N1 viruses from clade 1, 2.3.4 and 2.3.2 and agro-ecological factors. We found that the distribution of clades in the Mekong region from 2004 to 2013 was strongly regionalised, defining specific epidemiological zones, or epizones. Clade 1 became entrenched in the Mekong Delta and was not supplanted by newer clades, in association with a relatively higher presence of domestic ducks. In contrast, two new clades were introduced (2.3.4 and 2.3.2) in northern Viet Nam and were associated with higher chicken density and more intensive chicken production systems. We suggest that differences in poultry production systems in these different epizones may explain these associations, along with differences in introduction pressure from neighbouring countries. The different distribution patterns found at the clade level would not be otherwise apparent through analysis treating all outbreaks equally, which requires improved linking of disease outbreak records and genetic sequence data
The Detection of New Methanol Masers in the 5_{-1}-4_0E Line
Fifty-one objects in the 5_{-1}-4_0E methanol line at 84.5 GHz were detected
during a survey of Class I maser sources. Narrow maser features were found in
17 of these. Broad quasi-thermal lines were detected towards other sources. One
of the objects with narrow features, the young bipolar outflow L 1157 was also
observed in the 8_0-7_1A+ line at 95.2 GHz; a narrow line was detected at this
frequency. Analysis showed that the broad lines are usually inverted. The
quasi-thermal profiles imply that the line opacities are not larger than
several units. These results confirm the plausibility of models in which
compact Class I masers appear in extended sources as a result of an appropriate
velocity field.
Measurements of linear polarization at 84.5 GHz in 13 sources were made. No
polarization was found except a tentative detection of a weak polarization in M
8E
The effects of economic deprivation on psychological well-being among the working population of Switzerland
BACKGROUND: The association between poverty and mental health has been widely investigated. There is, however, limited evidence of mental health implications of working poverty, despite its representing a rapidly expanding segment of impoverished populations in many developed nations. In this study, we examined whether working poverty in Switzerland, a country with substantial recent growth among the working poor, was correlated with two dependent variables of interest: psychological health and unmet mental health need. METHODS: This cross-sectional study used data drawn from the first 3 waves (1999–2001) of the Swiss Household Panel, a nationally representative sample of the permanent resident population of Switzerland. The study sample comprised 5453 subjects aged 20–59 years. We used Generalized Estimating Equation models to investigate the association between working poverty and psychological well-being; we applied logistic regression models to analyze the link between working poverty and unmet mental health need. Working poverty was represented by dummy variables indicating financial deficiency, restricted standard of living, or both conditions. RESULTS: After controlling other factors, restricted standard of living was significantly (p < .001) negatively correlated with psychological well-being; it was also associated with approximately 50% increased risk of unmet mental health need (OR = 1.55; 95% CI 1.17 – 2.06). CONCLUSION: The findings of this study contribute to our understanding of the potential psychological impact of material deprivation on working Swiss citizens. Such knowledge may aid in the design of community intervention programs to help reduce the individual and societal burdens of poverty in Switzerland
Acute neuropsychological effects of MDMA and ethanol (co-)administration in healthy volunteers
Contains fulltext :
73592.pdf (publisher's version ) (Open Access)RATIONALE: In Western societies, a considerable percentage of young people expose themselves to 3,4-methylenedioxymethamphetamine (MDMA or "ecstasy"). Commonly, ecstasy is used in combination with other substances, in particular alcohol (ethanol). MDMA induces both arousing as well as hallucinogenic effects, whereas ethanol is a general central nervous system depressant. OBJECTIVE: The aim of the present study is to assess the acute effects of single and co-administration of MDMA and ethanol on executive, memory, psychomotor, visuomotor, visuospatial and attention function, as well as on subjective experience. MATERIALS AND METHODS: We performed a four-way, double-blind, randomised, crossover, placebo-controlled study in 16 healthy volunteers (nine male, seven female) between the ages of 18-29. MDMA was given orally (100 mg) and blood alcohol concentration was maintained at 0.6 per thousand by an ethanol infusion regime. RESULTS: Co-administration of MDMA and ethanol was well tolerated and did not show greater impairment of performance compared to the single-drug conditions. Impaired memory function was consistently observed after all drug conditions, whereas impairment of psychomotor function and attention was less consistent across drug conditions. CONCLUSIONS: Co-administration of MDMA and ethanol did not exacerbate the effects of either drug alone. Although the impairment of performance by all drug conditions was relatively moderate, all induced significant impairment of cognitive function
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