The Concurrent Validity of the Shipley-2 and the WAIS-IV

The recently developed Shipley-2 was compared to the Wechsler Adult Intelligence Scale, 4th edition (WAIS-IV) in order to determine the former\u27s level of concurrent validity as a test of intellectual ability. A convenience sample of 25 clinical subjects were administered both measures at two participating outpatient clinics, and the sum results of this testing were tabulated and then correlated through the use of a statistical software package. Results showed very strong levels of correlation between the five Shipley-2 scores (Vocabulary, Abstraction, Block Patterns, Composite A, and Composite B) and the Full Scale IQ (FSIQ) of the WAIS-IV (r = .549 to .807, p = .01), as well as moderate to strong correlations between the Shipley-2 scale scores and the Index scores from the WAIS-IV. More varied levels of correlation were detected between the WAIS-IV subtests and the various scores from the Shipley-2. These results suggest that the Shipley-2 can be effectively used as a screening tool or quick measure of intellectual ability among an outpatient clinical population or within similar mental health settings. However, a larger and more comprehensive analysis is needed in order to determine the full range of the new Shipley\u27s applicability as a measure of intellectual functioning

Cryptococcus neoformans chitin synthase 3 plays a critical role in dampening host inflammatory responses

Cryptococcus neoformans is the most common disseminated fungal pathogen in AIDS patients, resulting in ∼200,000 deaths each year. There is a pressing need for new treatments for this infection, as current antifungal therapy is hampered by toxicity and/or the inability of the host’s immune system to aid in resolution of the disease. An ideal target for new therapies is the fungal cell wall. The cryptococcal cell wall is different from the cell walls of many other pathogenic fungi in that it contains chitosan. Strains that have decreased chitosan are less pathogenic and strains that are deficient in chitosan are avirulent and can induce protective responses. In this study, we investigated the host responses to a chs3Δ strain, a chitosan-deficient strain, and found that mice inoculated with the chs3Δ strain all died within 36 h and that death was associated with an aberrant hyperinflammatory immune response driven by neutrophils, indicating that chitosan is critical in modulating the immune response to Cryptococcus.Cryptococcus neoformans infections are significant causes of morbidity and mortality among AIDS patients and the third most common invasive fungal infection in organ transplant recipients. One of the main interfaces between the fungus and the host is the fungal cell wall. The cryptococcal cell wall is unusual among human-pathogenic fungi in that the chitin is predominantly deacetylated to chitosan. Chitosan-deficient strains of C. neoformans were found to be avirulent and rapidly cleared from the murine lung. Moreover, infection with a chitosan-deficient C. neoformans strain lacking three chitin deacetylases (cda1Δcda2Δcda3Δ) was found to confer protective immunity to a subsequent challenge with a virulent wild-type counterpart. In addition to the chitin deacetylases, it was previously shown that chitin synthase 3 (Chs3) is also essential for chitin deacetylase-mediated formation of chitosan. Mice inoculated with the chs3Δ strain at a dose previously shown to induce protection with the cda1Δcda2Δcda3Δ strain die within 36 h after installation of the organism. Mortality was not dependent on viable fungi, as mice inoculated with a heat-killed preparation of the chs3Δ strain died at the same rate as mice inoculated with a live chs3Δ strain, suggesting that the rapid onset of death was host mediated, likely caused by an overexuberant immune response. Histology, cytokine profiling, and flow cytometry indicate a massive neutrophil influx in the mice inoculated with the chs3Δ strain. Mice depleted of neutrophils survived chs3Δ inoculation, indicating that death was neutrophil mediated. Altogether, these studies lead us to conclude that Chs3, along with chitosan, plays critical roles in dampening cryptococcus-induced host inflammatory responses

An investigation op the electrical resistance of thin films of rare earth metals

Measurements have been made of the electrical resistivity in the film plane of rare earth metal films over a thickness range of 130 A to 250O A. The films are deposited onto glass substrates by high vacuum evaporation at pressures better than 10(^-6) torr and are protected by an over coating of silicon monoxide. The resistivity has been examined between 4.2 K and room temperature; the magnetoresistance has also been studied in the presence of applied transverse fields up to 15 KOe and longitudinal fields up to 10 KOe. The variation of resistance with temperature is similar in form to that obtained for bulk specimens for all film thicknesses. However, in films less than about 400 A thick, the spin-disorder resistivity shows a pronounced decrease in magnitude but the degree of variation did not appear to be consistent with thickness. The spin-disorder resistivity is obtained by computing a least-squares fit to the linear high temperature part of the resistance curve and extrapolating to 0 K. The results show that in the temperature region where spin-wave theory is applicable, at very low temperatures pspin ɚ T(^2) in accordance with spin-wave theory but at higher temperatures, this falls off, so that It is found that pspin ɚ T(^2) in the first case, the variation of Pspin(T) can be better explained in terms of an anisotropy energy gap and the change to a T(^3/2) proportionality has been related to the behaviour of the spontaneous magnetization and the anomalous Hall effect. In addition, the variation of pSpin of dysprosium is examined in detail and it is suggested that the observed fall off of spin resistivity with thickness, which can be extrapolated to zero at zero thickness, is related to the variation of magnetization with thickness in the film. Finally, magnetoresistive studies detected negative effects with fields applied transversely to the electric field. The anomalous peak at the Neel point is suppressed in dysprosium and terbium and a small demagnetizing field effect is observed in dysprosium

A metabolomic investigation of the effects of vitamin E supplementation in humans.

RIGHTS : This article is licensed under the BioMed Central licence at http://www.biomedcentral.com/about/license which is similar to the 'Creative Commons Attribution Licence'. In brief you may : copy, distribute, and display the work; make derivative works; or make commercial use of the work - under the following conditions: the original author must be given credit; for any reuse or distribution, it must be made clear to others what the license terms of this work are.BACKGROUND: Vitamin E is a nutrient with both antioxidant and non-antioxidant activities and has been shown to modulate the function of a number of cell types in vitro and in human studies. However studies have also shown vitamin E to have detrimental interactions and therefore it is important to establish the extent to which this nutrient influences metabolism. Metabolomics can potentially identify nutrient-metabolism interactions and therefore the aim of this study was to use a non-targeted metabolomic approach to identify changes to the plasma metabolome following vitamin E supplementation in humans. METHODS: A relatively homogenous healthy adult male population (n = 10) provided a fasting blood sample immediately before and after a 4-week vitamin E supplementation regime (400 mg/d of RRR-α-tocopheryl acetate)) on top of their habitual diet. Plasma samples were analysed for vitamin E and clinical markers. Plasma underwent non-targeted metabolite profiling using liquid chromatography/mass spectroscopy and data was processed using multivariate statistical analysis. RESULTS: Plasma vitamin E concentrations were significantly increased following supplementation (p < 0.001). A partial least squares-discriminant analysis (PLS-DA) model was able to discriminate between samples taken pre and post vitamin E supplementation (goodness of fit R2Y = 0.82, predictive ability Q2 = 0.50). Variable influence on projection and PLS-DA loadings highlighted a number of discriminating ions that were confirmed as discriminatory through pairwise analysis. From database searches and comparison with standards these metabolites included a number of lysophosphatidylcholine species (16:0, 18:0, 18:1, 18:2, 20:3 and 22:6) that were increased in intensity post supplementation by varying degrees from 4% to 29% with the greatest changes found for lysoPC 22:6 and 20:3. CONCLUSIONS: Although a small scale study, these results potentially indicate that vitamin E supplementation influences phospholipid metabolism and induces lysoPC generation; a general pro-inflammatory response. Moreover the study identifies novel areas of vitamin E interactions and highlights the potential of metabolomics for elucidating interactions between nutrients and metabolic pathways in nutritional research

Sustainable Grazing Systems- A Program to Develop and Deliver Improved Temperate Pastures in Australia

The Sustainable Grazing Systems Program aims to combine the efforts of producers, researchers and extension agents into a focused partnership to develop, manage and implement grazing systems that are more profitable and more sustainable. Rather than the traditional approach of undertaking the research and then developing extension packages for livestock producers, this Program has set up a network with producers, researchers and extension agents to collectively develop and test improved systems. The process is described as colearning. Compared with more traditional approaches, producer input is greatly increased as the role of researchers and extension agents is modified, but not decreased. While there is substantial input into the Program from research and extension groups, this paper focuses on the role and input of producers

Symposium 2: Modern approaches to nutritional research challenges: Targeted and non-targeted approaches for metabolite profiling in nutritional research

The present report discusses targeted and non-targeted approaches to monitor single nutrients and global metabolite profiles in nutritional research. Non-targeted approaches such as metabolomics allow for the global description of metabolites in a biological sample and combine an analytical platform with multivariate data analysis to visualise patterns between sample groups. In nutritional research metabolomics has generated much interest as it has the potential to identify changes to metabolic pathways induced by diet or single nutrients, to explore relationships between diet and disease and to discover biomarkers of diet and disease. Although still in its infancy, a number of studies applying this technology have been performed; for example, the first study in 2003 investigated isoflavone metabolism in females, while the most recent study has demonstrated changes to various metabolic pathways during a glucose tolerance test. As a relatively new technology metabolomics is faced with a number of limitations and challenges including the standardisation of study design and methodology and the need for careful consideration of data analysis, interpretation and identification. Targeted approaches are used to monitor single or multiple nutrient and/or metabolite status to obtain information on concentration, absorption, distribution, metabolism and elimination. Such applications are currently widespread in nutritional research and one example, using stable isotopes to monitor nutrient status, is discussed in more detail. These applications represent innovative approaches in nutritional research to investigate the role of both single nutrients and diet in health and disease

Cryptococcus at work: Gene expression during human infection

Meningitis is a frequent manifestation of infection due to Cryptococcus neoformans and a major cause of increased morbidity in patients with AIDS. Numerous in vitro gene expression and genetic studies of the fungus have predicted a myriad of genes, pathways, and biological processes that may be critical for pathogenesis, and many studies using animal models have supported the role of these processes during infection. However, the relevance of these hypotheses based on in vitro and animal models has often been questioned. A recent study by Chen et al. [Y. Chen, D. L. Toffaletti, J. L. Tenor, A. P. Litvintseva, C. Fang, T. G. Mitchell, T. R. McDonald, K. Nielsen, D. R. Boulware, T. Bicanic, and J. R. Perfect, mBio 5(1):e01087-13, 2014] represents an important step in understanding the cryptococcal response during human infection

Supporting novel home network management interfaces with Openflow and NOX

The Homework project has examined redesign of existing home network infrastructures to better support the needs and requirements of actual home users. Integrating results from several ethnographic studies, we have designed and built a home networking platform providing detailed per-flow measurement and management capabilities supporting several novel management interfaces. This demo specifically shows these new visualization and control interfaces, and describes the broader benefits of taking an integrated view of the networking infrastructure, realised through our router's augmented measurement and control APIs. Aspects of this work have been published: the Homework Database in Internet Management (IM) 2011 and implications of the ethnographic results are to appear at the SIGCOMM W-MUST workshop 2011. Separate, more detailed expositions of the interface elements and system performance and implications are currently under submission at other venues. A partial code release is already available and we anticipate fuller public beta release by Q4 2011