Cap completion and C-terminal repeat domain kinase recruitment underlie the initiation-elongation transition of RNA polymerase II.

After transcription initiation, RNA polymerase (Pol) II escapes from the promoter and recruits elongation factors. The molecular basis for the initiation-elongation factor exchange during this transition remains poorly understood. Here, we used chromatin immunoprecipitation (ChIP) to elucidate the initiation-elongation transition of Pol II in the budding yeast Saccharomyces cerevisiae. We show that the early Pol II elongation factor Spt5 contributes to stable recruitment of the mRNA capping enzymes Cet1, Ceg1, and Abd1. Genome-wide occupancy for Cet1 and Ceg1 is restricted to the transcription start site (TSS), whereas occupancy for Abd1 peaks at110 nucleotides downstream, and occupancy for the cap-binding complex (CBC) rises subsequently. Abd1 and CBC are important for recruitment of the kinases Ctk1 and Bur1, which promote elongation and capping enzyme release. These results suggest that cap completion stimulates productive Pol II elongation

Probing Z' gauge boson with the spin configuration of top quark pair production at future e−e+e^- e^+ linear colliders

We explore the effects of extra neutral gauge boson involved in the supersymmetric E6 model on the spin configuration of the top quark pair produced at the polarized e- e+ collider. Generic mixing terms are considered including kinetic mixing terms as well as mass mixing. In the off-diagonal spin basis of the standard model, we show that the cross sections for the suppressed spin configurations can be enhanced with the effects of the Z' boson through the modification of the spin configuration of produced top quark pair enough to be measured in the Linear Colliders, which provides the way to observe the effects of Z' boson and discriminate the pattern of gauge group decomposition. It is pointed out that the kinetic mixing may dilute the effects of mass mixing terms, and we have to perform the combined analysis.Comment: 19 pages including 5 figures, ReVTe

Complexity of Bradley-Manna-Sipma Lexicographic Ranking Functions

In this paper we turn the spotlight on a class of lexicographic ranking functions introduced by Bradley, Manna and Sipma in a seminal CAV 2005 paper, and establish for the first time the complexity of some problems involving the inference of such functions for linear-constraint loops (without precondition). We show that finding such a function, if one exists, can be done in polynomial time in a way which is sound and complete when the variables range over the rationals (or reals). We show that when variables range over the integers, the problem is harder -- deciding the existence of a ranking function is coNP-complete. Next, we study the problem of minimizing the number of components in the ranking function (a.k.a. the dimension). This number is interesting in contexts like computing iteration bounds and loop parallelization. Surprisingly, and unlike the situation for some other classes of lexicographic ranking functions, we find that even deciding whether a two-component ranking function exists is harder than the unrestricted problem: NP-complete over the rationals and $\Sigma^P_2$-complete over the integers.Comment: Technical report for a corresponding CAV'15 pape

On the phenomenology of a Z' coupling only to third-family fermions

The phenomenology of an additional U(1) neutral gauge boson Z' coupled to the third family of fermions is discussed. One might expect such a particle to contribute to processes where taus, b and t quarks are produced. Precision data from LEP1 put severe constraints on the mixing and heavy-boson mass. We find that the effects of such a particle could not be observed at hadronic colliders, be it at the Tevatron or the LHC, because of the QCD background. At LEP2 and future e^+e^- linear colliders, one could instead hope to observe such effects, in particular for b\bar b final states.Comment: 36 pages, LaTeX, including 12 figure

Indications for an Extra Neutral Gauge Boson in Electroweak Precision Data

A new analysis of the hadronic peak cross section at LEP 1 implies a small amount of missing invisible width in Z decays, while the effective weak charge in atomic parity violation has been determined recently to 0.6% accuracy, indicating a significantly negative S parameter. As a consequence of these two deviations, the data are described well if the presence of an additional Z' boson, such as predicted in Grand Unified Theories, is assumed. Moreover, the data are now rich enough to study an arbitrary extra Z' boson and to determine its couplings in a model independent way. An excellent best fit to the data is obtained in this case, suggesting the possibility of a family non-universal Z' with properties similar to ones predicted in a class of superstring theories.Comment: 5 pages of ReVTeX, 2 figure

Nonexotic Neutral Gauge Bosons

We study theoretical and experimental constraints on electroweak theories including a new color-singlet and electrically-neutral gauge boson. We first note that the electric charges of the observed fermions imply that any such Z' boson may be described by a gauge theory in which the Abelian gauge groups are the usual hypercharge along with another U(1) component in a kinetic-diagonal basis. Assuming that the observed quarks and leptons have generation-independent U(1) charges, and that no new fermions couple to the standard model gauge bosons, we find that their U(1) charges form a two-parameter family consistent with anomaly cancellation and viable fermion masses, provided there are at least three right-handed neutrinos. We then derive bounds on the Z' mass and couplings imposed by direct production and Z-pole measurements. For generic charge assignments and a gauge coupling of electromagnetic strength, the strongest lower bound on the Z' mass comes from Z-pole measurements, and is of order 1 TeV. If the new U(1) charges are proportional to B-L, however, there is no tree-level mixing between the Z and Z', and the best bounds come from the absence of direct production at LEPII and the Tevatron. If the U(1) gauge coupling is one or two orders of magnitude below the electromagnetic one, these bounds are satisfied for most values of the Z' mass.Comment: 26 pages, 2 figures. A comparison with the LEP bounds on sneutrino resonances is include

Reconstruction of the Extended Gauge Structure from Z′Z' Observables at Future Colliders

The discovery of a new neutral gauge boson $Z'$ with a mass in the TeV region would allow for determination of gauge couplings of the $Z'$ to ordinary quarks and leptons in a model independent way. We show that these couplings in turn would allow us to determine the nature of the extended gauge structure. As a prime example we study the $E_6$ group. In this case two discrete constraints on experimentally determined couplings have to be satisfied. If so, the couplings would then uniquely determine the two parameters, $\tan \beta$ and $\delta$, which fully specify the nature of the $Z'$ within $E_6$. If the $Z'$ is part of the $E_6$ gauge structure, then for $M_{Z'}=1$ TeV $\tan \beta$ and $\delta$ could be determined to around $10\%$ at the future colliders. The NLC provides a unique determination of the two constraints as well as of $\tan \beta$ and $\delta$, though with slightly larger error bars than at the LHC. On the other hand, since the LHC primarily determines three out of four normalized couplings, it provides weaker constraints for the underlying gauge structure.Comment: 14 pages LaTeX using RevTeX and psfig.sty. TeX source and 3 PS figures, tarred, compressed and uuencoded; also available via anonymous ftp to ftp://dept.physics.upenn.edu/pub/Cvetic/UPR-636-T

Spatially Resolved Ultraviolet Spectroscopy of the Great Dimming of Betelgeuse

The bright supergiant, Betelgeuse (Alpha Orionis, HD 39801) experienced a visual dimming during 2019 December and the first quarter of 2020 reaching an historic minimum 2020 February 7$-$13. During 2019 September-November, prior to the optical dimming event, the photosphere was expanding. At the same time, spatially resolved ultraviolet spectra using the Hubble Space Telescope/Space Telescope Imaging Spectrograph revealed a substantial increase in the ultraviolet spectrum and Mg II line emission from the chromosphere over the southern hemisphere of the star. Moreover, the temperature and electron density inferred from the spectrum and C II diagnostics also increased in this hemisphere. These changes happened prior to the Great Dimming Event. Variations in the Mg II k-line profiles suggest material moved outwards in response to the passage of a pulse or acoustic shock from 2019 September through 2019 November. It appears that this extraordinary outflow of material from the star, likely initiated by convective photospheric elements, was enhanced by the coincidence with the outward motions in this phase of the $\sim$400 day pulsation cycle. These ultraviolet observations appear to provide the connecting link between the known large convective cells in the photosphere and the mass ejection event that cooled to form the dust cloud in the southern hemisphere imaged in 2019 December, and led to the exceptional optical dimming of Betelgeuse in 2020 February.Comment: 11 pages, 8 figures, Astrophysical Journal, accepte

Z' Bosons at Colliders: a Bayesian Viewpoint

We revisit the CDF data on di-muon production to impose constraints on a large class of Z' bosons occurring in a variety of E_6 GUT based models. We analyze the dependence of these limits on various factors contributing to the production cross-section, showing that currently systematic and theoretical uncertainties play a relatively minor role. Driven by this observation, we emphasize the use of the Bayesian statistical method, which allows us to straightforwardly (i) vary the gauge coupling strength, g', of the underlying U(1)'; (ii) include interference effects with the Z' amplitude (which are especially important for large g'); (iii) smoothly vary the U(1)' charges; (iv) combine these data with the electroweak precision constraints as well as with other observables obtained from colliders such as LEP 2 and the LHC; and (v) find preferred regions in parameter space once an excess is seen. We adopt this method as a complementary approach for a couple of sample models and find limits on the Z' mass, generally differing by only a few percent from the corresponding CDF ones when we follow their approach. Another general result is that the interference effects are quite relevant if one aims at discriminating between models. Finally, the Bayesian approach frees us of any ad hoc assumptions about the number of events needed to constitute a signal or exclusion limit for various actual and hypothetical reference energies and luminosities at the Tevatron and the LHC.Comment: PDFLaTeX, 24 pages, 7 figures. Version with improved tables and figure